Achievement of genetics in plant reproduction research: the past decade for the coming decade

Suwabe, Keita; Suzuki, Go; Watanabe, Masao

doi:10.1266/ggs.85.297

Cited by 11 publications

(6 citation statements)

References 200 publications

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“…Reproduction is a central process for all organisms to pass on their genome to the next generation. Plants have evolved fertilization systems between male and female gametophytes (Suwabe et al, 2010). In angiosperms, the male gametophyte is produced as pollen, which develops in the anther of the stamen.…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in Arabidopsis thaliana

et al. 2018

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Stigmatic papillae develop at the apex of the gynoecium and play an important role as a site of pollination. The papillae in Brassicaceae are of the dry and unicellular type, and more than 15,000 genes are expressed in the papillae; however, the molecular and physiological mechanisms of their development remain unknown. We found that the papillae in Arabidopsis thaliana change their length in response to altered ambient humidity: papillae of flowers incubated under high humidity elongated more than those under normal humidity conditions. Genetic analysis and transcriptome data suggest that an abscisic acid-mediated abiotic stress response mechanism regulates papilla length. Our data suggest a flexible regulation of papilla elongation at the post-anthesis stage, in response to abiotic stress, as an adaptation to environmental conditions.

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Section: Introductionmentioning

confidence: 99%

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in Arabidopsis thaliana

et al. 2018

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“…Flowering plants have evolved exquisite systems for the control of mating interactions before fertilization, and the most extensively studied of these is self-incompatibility (SI) (Suwabe et al 2010, Watanabe et al 2012). SI is a genetic system for intraspecific pollen selectivity and is recognized as the most sophisticated system to prevent selfing (Bateman 1995).…”

Section: Introductionmentioning

confidence: 99%

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Osaka

Matsuda

Sakazono

et al. 2013

Plant and Cell Physiology

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Pollination is an early and critical step in plant reproduction, leading to successful fertilization. It consists of many sequential processes, including adhesion of pollen grains onto the surface of stigmatic papilla cells, foot formation to strengthen pollen–stigma interaction, pollen hydration and germination, and pollen tube elongation and penetration. We have focused on an examination of the expressed genes in papilla cells, to increase understanding of the molecular systems of pollination. From three representative species of Brassicaceae (Arabidopsis thaliana, A. halleri and Brassica rapa), stigmatic papilla cells were isolated precisely by laser microdissection, and cell type-specific gene expression in papilla cells was determined by RNA sequencing. As a result, 17,240, 19,260 and 21,026 unigenes were defined in papilla cells of A. thaliana, A. halleri and B. rapa, respectively, and, among these, 12,311 genes were common to all three species. Among the17,240 genes predicted in A. thaliana, one-third were papilla specific while approximately half of the genes were detected in all tissues examined. Bioinformatics analysis revealed that genes related to a wide range of reproduction and development functions are expressed in papilla cells, particularly metabolism, transcription and membrane-mediated information exchange. These results reflect the conserved features of general cellular function and also the specific reproductive role of papilla cells, highlighting a complex cellular system regulated by a diverse range of molecules in these cells. This study provides fundamental biological knowledge to dissect the molecular mechanisms of pollination in papilla cells and will shed light on our understanding of plant reproduction mechanisms.

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“…SI in Brassica species is controlled by a single locus, termed the S-locus, with multiple alleles (Bateman, 1955;Nou et al, 1993). From several molecular analyses, three genes at the S-locus were characterized, which are involved in self/non-self pollen recognition and the subsequent downstream reaction (reviewed by Suwabe et al, 2010;Watanabe et al, 2012;Doucet et al, 2016). The female S-determinant, a receptor-type protein kinase (S-receptor kinase; SRK), consisting of an extracellular domain (S-domain, also known as SLG homologous domain), a transmembrane domain and a serine/threonine kinase domain, has been identified and characterized (Stein et al, 1991;Watanabe et al, 1994;Takasaki et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in Brassica rapa L. toward identification of a novel self-incompatibility factor

et al. 2019

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Self-incompatibility (SI) is a sophisticated system for pollen selectivity to prevent pollination by genetically identical pollen. In Brassica, it is genetically controlled by a single, highly polymorphic S-locus, and the male and female S-determinant factors have been identified as S-locus protein 11 (SP11)/S-locus cysteine-rich protein (SCR) and S-locus receptor kinase (SRK), respectively. However, the overall molecular system and identity of factors in the downstream cascade of the SI reaction remain unclear. Previously, we identified a self-compatible B. rapa mutant line, TSC28, which has a disruption in an unidentified novel factor of the SI signaling cascade. Here, in a genetic analysis of TSC28, using an F 2 population from a cross with the reference B. rapa SI line Chiifu-401, the causal gene was mapped to a genetic region of DNA containing markers BrSA64 and ACMP297 in B. rapa chromosome A1. By fine mapping using an F 2 population of 1,034 plants, it was narrowed down to a genetic region between DNA markers ACMP297 and BrgMS4028, with physical length approximately 1.01 Mbp. In this genomic region, 113 genes are known to be located and, among these, we identified 55 genes that were expressed in the papilla cells. These are candidates for the gene responsible for the disruption of SI in TSC28. This list of candidate genes will contribute to the discovery of a novel downstream factor in the SP11-SRK signaling cascade in the Brassica SI system.

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Achievement of genetics in plant reproduction research: the past decade for the coming decade

Cited by 11 publications

References 200 publications

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells From a Combination of Laser Microdissection and RNA Sequencing

Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor

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