Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in &lt;i&gt;Arabidopsis thaliana&lt;/i&gt;

Takeda, Seiji; Ochiai, Kohki; Kagaya, Yasuaki; Egusa, Wataru; Morimoto, Hiroaki; Sakazono, Satomi; Osaka, Mariko; Nabemoto, Moe; Suzuki, Go; Watanabe, Masao; Suwabe, Keita

doi:10.1266/ggs.18-00025

Cited by 4 publications

(7 citation statements)

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“…Moreover, hypothesis suggested in our study might be also applied for other plant species, because molecular mechanisms underlying development of reproductive organs are conserved in various species including trees to some extent (Yoo et al, 2012;Cronk et al, 2020). A previous study demonstrated that elongation of stigmatic papillae can be also induced by high humidity (Takeda et al, 2018). In contrast to our study, enlargement of stigma induced by high humidity was not associated with pollen attachment (Takeda et al, 2018), suggesting that effects of pollen attachment on stigmatic development might be different depending on humidity.…”

Section: Discussionmentioning

confidence: 70%

“…For example, a previous study demonstrated abnormal anther development, lower pollen viability, reduced filament elongation, ovule abortion and failure of flowering in Arabidopsis plants under drought (Su et al, 2013). In contrast, enhanced elongation of stigmatic papillae was observed under high humidity (Takeda et al, 2018). These studies suggest that water status in surrounding environments can alter the developmental patterns of reproductive organs, leading to decrease in seed production.…”

Section: Introductionmentioning

confidence: 88%

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Failure of Pollen Attachment to the Stigma Triggers Elongation of Stigmatic Papillae in Arabidopsis thaliana

Katano

Suzuki

2020

Front. Plant Sci.

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Pollination is one of key determinants of yield production in important crops, such as grains and beans in which seeds are utilized as agricultural products. Thus, to fulfil food demand for growing world population, it is necessary to elucidate the mechanisms that regulate pollination, leading to increase in yield production. In this study, we compared detailed morphological characteristics of reproductive organs in Arabidopsis thaliana grown under control conditions or subjected to heat stress. Shorter length of anthers, filaments, and petals were observed in plants subjected to heat stress compared to those under control conditions. In contrast, heat stress resulted in enlargement of stigma via elongation of stigmatic papillae. Classification of stigmas based on patterns of pollen attachment indicated that pollen attachment to stigma clearly decreased under heat stress. In addition, artificial pollination experiment demonstrated that stigma shrank when pollen attached, but, continued to enlarge in the absence of pollen. Such modulation of stigma size depending on the presence or absence of pollen was observed both under control and heat stressed conditions. Taken together, these results suggest that elongation of stigmatic papillae is associated with failure of pollen attachment to the stigma, rather than heat stress. Furthermore, histochemical staining experiments suggest that Ca 2+ derived from pollen together with O 2 might be associated with morphological alteration of stigma depending on the patterns of pollen attachment.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 88%

Failure of Pollen Attachment to the Stigma Triggers Elongation of Stigmatic Papillae in Arabidopsis thaliana

Katano

Suzuki

2020

Front. Plant Sci.

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“…We discovered a novel phenomenon that pollen attachment onto stigma is clearly associated with stigma shrinkage, and disturbance of pollen attachment onto stigma by heat stress results in elongation of stigmatic papillae. A previous study demonstrated that high humidity condition induced morphological change in stigmatic papillae via regulation of ABA signaling (Takeda et al, 2018). In our study, enlargement of stigmatic papillae was observed under heat stress, suggesting that abiotic stress response mediated by ABA, an important stress response plant hormone could be involved in stigma enlargement.…”

Section: Resultsmentioning

confidence: 99%

“…For instance, drought resulted in abnormal anther development, lower pollen viability, reduced filament elongation, ovule abortion and failure of flowering in Arabidopsis (Su et al, 2013). In contrast, enhanced elongation of stigmatic papillae was observed under high humidity (Takeda et al, 2018). These studies indicated that water status in surrounding environments alter the developmental patterns of reproductive organs, leading to decrease in seed production.…”

Section: Introductionmentioning

confidence: 99%

Elongation of stigmatic papillae induced by heat stress is associated with disturbance of pollen attachment inArabidopsis thaliana

Katano

Suzuki

2019

Preprint

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Heat stress can seriously impact on yield production and quality of crops. Many studies 31 uncovered the molecular mechanisms that regulate heat stress responses in plants. 32 Nevertheless, effects of heat stress on the morphology of plants were still not extensively 33 studied. In this study, we observed the detailed morphological changes of reproductive 34 organs in Arabidopsis thaliana caused by heat stress. Larger area of stigma, and shorter 35 length of anthers, filaments and petals were observed in plants subjected to heat stress 36 compared to those under controlled conditions. Scanning electron microscopy (SEM) 37 observation showed that length of stigmatic papillae without pollens seemed to be longer 38 than that with pollens. In addition, classification of stigmas based on pollen attachment 39 patterns together with artificial pollination assay revealed that pollen attachment onto 40 stigma was clearly decreased by heat stress, and indicated that heat induced elongation of 41 stigmatic papillae might be associated with disturbance of pollen attachment onto stigma. 42 Furthermore, histochemical staining experiments revealed that crosstalk between Ca 2+ 43 and NO derived from pollens and O2derived from stigma might be associated with 44 morphological alteration of stigma. 45 46 KEYWORD 47 Heat stress, morphology, SEM, pollen, stigmatic papillae 48 49 50 51 52 53 54 55 56 57 58 59 60 61 Negative effects of abiotic stresses on development of reproductive organs have 62 been reported in recent studies. For instance, drought resulted in abnormal anther 63 development, lower pollen viability, reduced filament elongation, ovule abortion and 64 failure of flowering in Arabidopsis (Su et al., 2013). In contrast, enhanced elongation of 65 stigmatic papillae was observed under high humidity (Takeda et al., 2018). These studies 66 indicated that water status in surrounding environments alter the developmental patterns 67 of reproductive organs, leading to decrease in seed production. Heat stress was also shown 68 to negatively affect seed production (Katano et al., 2018b; Suzuki et al., 2013; Tunc-69 Ozdemir et al., 2013). We can therefore expect that decrease in seed production caused 70 by heat stress might be at least partially attributed to morphological alterations in 71 reproductive organs. Indeed, it has been well known that heat stress dramatically impacts 72 on development of anthers and pollens (Katano et al., 2018a; Rieu and Twell, 2017). 73 However, effects of heat stress on development of other reproductive organs are still 74 largely unknown. In addition, only few studies focused on detailed morphological 75 alterations of reproductive organs caused by heat stress, despite the remarkable progress 76 of researches uncovering molecular mechanisms that regulate response in reproductive 77 organs to heat stress.78In this study, to dissect the morphological alteration of reproductive organs induced 79 by heat stress, we investigated the size of each reproductive organ in Arabidopsis plants 80 gro...

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“…RNA-seq has been performed on various tissues and/or organs of several plant species (Zenoni et al, 2010;Zhang et al, 2010;Tong et al, 2013;Kim et al, 2014). Recently, this methodology was applied in plant reproductive research to understand pollination mechanisms and reproductive organ development and differentiation (Osaka et al, 2013;Zhou et al, 2014;Matsuda et al, 2015;Xu et al, 2016;Zhang et al, 2017;Takeda et al, 2018). Thus, in combination with a genetic approach, RNA-seq data have potential for identification of the causal gene for the SC phenotype in TSC28.…”

Section: Introductionmentioning

confidence: 99%

Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor

et al. 2019

Self Cite

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Self-incompatibility (SI) is a sophisticated system for pollen selectivity to prevent pollination by genetically identical pollen. In Brassica, it is genetically controlled by a single, highly polymorphic S-locus, and the male and female S-determinant factors have been identified as S-locus protein 11 (SP11)/S-locus cysteine-rich protein (SCR) and S-locus receptor kinase (SRK), respectively. However, the overall molecular system and identity of factors in the downstream cascade of the SI reaction remain unclear. Previously, we identified a self-compatible B. rapa mutant line, TSC28, which has a disruption in an unidentified novel factor of the SI signaling cascade. Here, in a genetic analysis of TSC28, using an F 2 population from a cross with the reference B. rapa SI line Chiifu-401, the causal gene was mapped to a genetic region of DNA containing markers BrSA64 and ACMP297 in B. rapa chromosome A1. By fine mapping using an F 2 population of 1,034 plants, it was narrowed down to a genetic region between DNA markers ACMP297 and BrgMS4028, with physical length approximately 1.01 Mbp. In this genomic region, 113 genes are known to be located and, among these, we identified 55 genes that were expressed in the papilla cells. These are candidates for the gene responsible for the disruption of SI in TSC28. This list of candidate genes will contribute to the discovery of a novel downstream factor in the SP11-SRK signaling cascade in the Brassica SI system.

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Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>

Cited by 4 publications

References 36 publications

Failure of Pollen Attachment to the Stigma Triggers Elongation of Stigmatic Papillae in Arabidopsis thaliana

Failure of Pollen Attachment to the Stigma Triggers Elongation of Stigmatic Papillae in Arabidopsis thaliana

Elongation of stigmatic papillae induced by heat stress is associated with disturbance of pollen attachment inArabidopsis thaliana

Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor

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