Achilles tendinopathy and patellar tendon: integrated imaging

Mondillo, Fiorentino; Cantalupo, Teresa; Toro, Giuseppe; Iannelli, Michele; Toro, Gabriella

doi:10.23736/s0394-3410.20.03984-3

Cited by 2 publications

(3 citation statements)

References 11 publications

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“…These are characterized by both micro- and macro-structural tendon alterations, leading to prolonged mechanical pain. 1 – 4 …”

Section: Introductionmentioning

confidence: 99%

Effects of adipose-derived mesenchymal stem cell conditioned medium on human tenocytes exposed to high glucose

Trotta,

Itro,

Lepre

et al. 2024

Therapeutic Advances in Musculoskeletal

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Introduction: Diabetic tendinopathy is a common invalidating and challenging disease that may be treated using stem cells. However, the effects of adipose-derived mesenchymal stem cell conditioned medium (ASC-CM) in diabetic tendinopathy have never been explored. Objectives: The present study evaluated the effects of ASC-CM on morphology, cell viability, structure, and scratch wound closure of human tenocytes (HTNC) exposed to high glucose (HG). Design: Experimental study. Methods: HTNC were exposed to HG (25 mM) for 7, 14 and 21 days with or without ASC-CM for the last 24 h. CM was collected from 4 × 105 ASCs, centrifuged for 10 min at 200 g and sterilized with 0.22 μm syringe filter. Results: At 7 days, HG-HTNC had decreased cell viability [72 ± 2%, p < 0.01 versus normal glucose (NG)] compared to NG-HTNC (90 ± 5%). A further decrement was detected after 14 and 21 days (60 ± 4% and 60 ± 5%, both, p < 0.01 versus NG and p < 0.01 versus HG7). While NG-HTNC evidenced a normal fibroblast cell-like elongated morphology, HG-HTNC showed increased cell roundness. In contrast, HG-HTNC exposed to ASC-CM showed a significant increase in cell viability, an improved cell morphology and higher scratch wound closure at all HG time points. Moreover, the exposure to ASC-CM significantly increased thrombospondin 1 and transforming growth factor beta 1 (TGF-β1) content in HG-HTNC. The TGF-β1 elevation was paralleled by higher Collagen I and Vascular Endothelial Growth Factor in HG-HTNC. Conclusion: ASC-CM may restore the natural morphology, cell viability and structure of HTNC, promoting their scratch wound closure through TGF-β1 increase.

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“…These are characterized by both micro- and macro-structural tendon alterations, leading to prolonged mechanical pain. 1 – 4 …”

Section: Introductionmentioning

confidence: 99%

Effects of adipose-derived mesenchymal stem cell conditioned medium on human tenocytes exposed to high glucose

Trotta,

Itro,

Lepre

et al. 2024

Therapeutic Advances in Musculoskeletal

View full text Add to dashboard Cite

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“…Tendon disorders cause an invalidating musculoskeletal pain that represents a common reason for physician consultation [4]. They severely impact the patient's daily life, thus being associated with an increase in both direct and indirect social costs [5][6][7]. However, the prevalence of tendinopathies is probably underestimated, considering the increase in sport activities.…”

Section: Introductionmentioning

confidence: 99%

“…Clinically, tendinopathies are characterized by activity-related pain, localized tenderness, swelling and reduced performance [21]. However, it is not uncommon to find completely asymptomatic patients with complete tendon degeneration [6,7].…”

Section: Introductionmentioning

confidence: 99%

Why Use Adipose-Derived Mesenchymal Stem Cells in Tendinopathic Patients: A Systematic Review

et al. 2022

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The aim of the present systematic review was to provide a clear overview of the clinical current research progress in the use of adipose-derived mesenchymal stem cells (ASCs) as an effective therapeutic option for the management of tendinopathies, pathologies clinically characterized by persistent mechanical pain and structural alteration of the tendons. The review was carried out using three databases (Scopus, ISI Web of Science and PubMed) and analyzed records from 2013 to 2021. Only English-language papers describing the isolation and manipulation of adipose tissue as source of ASCs and presenting ASCs as treatment for clinical tendinopathies were included. Overall, seven clinical studies met the inclusion criteria and met the minimum quality inclusion threshold. Data extraction and quality assessment were performed by groups of three reviewers. The available evidence showed the efficacy and safety of ASCs treatment for tendinopathies, although it lacked a clear description of the biomolecular mechanisms underlying the beneficial properties of ASCs.

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Achilles tendinopathy and patellar tendon: integrated imaging

Cited by 2 publications

References 11 publications

Effects of adipose-derived mesenchymal stem cell conditioned medium on human tenocytes exposed to high glucose

Effects of adipose-derived mesenchymal stem cell conditioned medium on human tenocytes exposed to high glucose

Why Use Adipose-Derived Mesenchymal Stem Cells in Tendinopathic Patients: A Systematic Review

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