Acid-Alkali Extraction of Triterpene Acids from Poria and Preparative Separation by High-Speed Counter-Current Chromatography

Dong, Hao; Ren, Yan; Li, Hua; Wang, Weihao; Xiao, Wang; Yang, Bin

doi:10.1080/01496395.2014.943769

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…The 1 H- and 13 C-NMR spectra revealed H-11 (δ 5.21)/C-11 (δ 120.7), H-7 (δ 5.17)/C-7 (δ 119.0), C-9 (δ 138.3), C-8 (δ 142.3), H-18 (δ 0.99), H-19 (δ 0.93), H-26 (δ 1.50), H-27 (δ 1.58), H-29 (δ 1.68), H-30 (δ 1.50), H-16 (δ 4.54), H-28 (δ 4.67, 4.76), H-24 (δ 5.27), C-3 (δ 178.3), C-21 (δ 180.0). Based on the structural features of triterpene compounds and the data given in the literature, compound- 1 was identified as poricoic acid B [ 1 , 17 , 20 , 22 , 23 , 24 ]. Poricoic acid B belongs to the 3,4-seco-lanostan-7,9(11)-diene type triterpenes [ 4 ].…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Zhang

Yin

Feng

et al. 2021

Foods

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In this study, triterpenoid compounds from Poriae Cutis were separated by high-speed countercurrent chromatography (HSCCC) and identified using ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS) and nuclear magnetic resonance (NMR). The in vitro anti-inflammatory activities of the purified triterpenoids on RAW 264.7 cells were also investigated. Triterpenoids, poricoic acid B, poricoic acid A, dehydrotrametenolic acid, and dehydroeburicoic acid were obtained; their levels of purity were 90%, 92%, 93%, and 96%, respectively. The results indicated that poricoic acid B had higher anti-inflammatory activity than those of poricoic acid A by inhibiting the generation of NO in lipopolysaccharide (LPS)-induced RAW 264.7 cells. However, dehydrotrametenolic acid and dehydroeburicoic acid had no anti-inflammatory activity. In addition, the production of cytokines (TNF-α, IL-1β, and IL-6) in cells treated with poricoic acid B decreased in a dose-dependent manner in the concentration range from 10 to 40 μg/mL. The results provide evidence for the use of Poriae Cutis as a natural anti-inflammatory agent in medicines and functional foods.

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Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Zhang

Yin

Feng

et al. 2021

Foods

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“…In terms of the five triterpenes obtained in the experiment, the open-loop structure had a more significant inhibitory effect on cells than the closed-loop structure. The hydroxyl group at the C16 position can increase the toxicity of triterpenes to tumor cells [24]. Studies have also shown that double bonds in the B and C rings could increase cytotoxicity [6].…”

Section: Antiproliferative Activity Of Paa and Pab On Human Cancer Cellsmentioning

confidence: 99%

Regulation of Tumor Apoptosis of Poriae cutis-Derived Lanostane Triterpenes by AKT/PI3K and MAPK Signaling Pathways In Vitro

Yue,

Feng,

Cai

et al. 2023

Nutrients

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Poria cocos is traditionally used as both food and medicine. Triterpenoids in Poria cocos have a wide range of pharmacological activities, such as diuretic, sedative and tonic properties. In this study, the anti-tumor activities of poricoic acid A (PAA) and poricoic acid B (PAB), purified by high-speed counter-current chromatography, as well as their mechanisms and signaling pathways, were investigated using a HepG2 cell model. After treatment with PAA and PAB on HepG2 cells, the apoptosis was obviously increased (p < 0.05), and the cell cycle arrested in the G2/M phase. Studies showed that PAA and PAB can also inhibit the occurrence and development of tumor cells by stimulating the generation of ROS in tumor cells and inhibiting tumor migration and invasion. Combined Polymerase Chain Reaction and computer simulation of molecular docking were employed to explore the mechanism of tumor proliferation inhibition by PAA and PAB. By interfering with phosphatidylinositol-3-kinase/protein kinase B, Mitogen-activated protein kinases and p53 signaling pathways; and further affecting the expression of downstream caspases; matrix metalloproteinase family, cyclin-dependent kinase -cyclin, Intercellular adhesion molecules-1, Vascular Cell Adhesion Molecule-1 and Cyclooxygenase -2, may be responsible for their anti-tumor activity. Overall, the results suggested that PAA and PAB induced apoptosis, halted the cell cycle, and inhibited tumor migration and invasion through multi-pathway interactions, which may serve as a potential therapeutic agent against cancer.

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Enrichment and separation of antitumor triterpene acids from the epidermis ofPoria cocosby pH-zone-refining counter-current chromatography and conventional high-speed counter-current chromatography

Dong

Ren

et al. 2015

J. Sep. Science

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Triterpene acids were extracted from the epidermis of Poria cocos (Schw.) Wolf. These acids were found to inhibit the growth of lung cancer cells in vitro and in vivo. An efficient method for the preparative separation of antitumor triterpene acids was established that involves the combination of pH-zone-refining counter-current chromatography and conventional high-speed counter-current chromatography. We used pH-zone-refining counter-current chromatography to concentrate the triterpene acids using a two-phase solvent system composed of petroleum ether/ethyl acetate/methanol/water (3:7:5:5, v/v/v/v), trifluoroacetic acid (10 mM) was added to the upper phase as a retainer, and ammonia (10 mM) was added to the lower phase as an eluter. As a result, 200 mg concentrate of triterpene acids was obtained from 1.0 g of crude extract. The concentrate was further separated by conventional high-speed counter-current chromatography using a solvent system composed of petroleum ether/ethyl acetate/methanol/water (0.8:1.2:1.2:0.9, v/v), yielding 50 mg of poricoic acid A and 5 mg of poricoic acid B from 120 mg concentrate, respectively. The inhibitory activity of the major compound on lung A549 cells was examined and poricoic acid A was found to significantly inhibit the growth of A 549 cells.

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Acid-Alkali Extraction of Triterpene Acids from Poria and Preparative Separation by High-Speed Counter-Current Chromatography

Cited by 4 publications

References 16 publications

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Anti-Inflammatory Activity of Four Triterpenoids Isolated from Poriae Cutis

Regulation of Tumor Apoptosis of Poriae cutis-Derived Lanostane Triterpenes by AKT/PI3K and MAPK Signaling Pathways In Vitro

Enrichment and separation of antitumor triterpene acids from the epidermis ofPoria cocosby pH-zone-refining counter-current chromatography and conventional high-speed counter-current chromatography

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