Acid and Alkaline Phosphatase in Serum

Walter, Klaus; Schütt, Christian

doi:10.1016/b978-0-12-091302-2.50067-0

Cited by 196 publications

(91 citation statements)

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“…Enzyme assay was carried out under optimal conditions. Alkaline phosphatase activity was determined using the method of Walter and Schutt (17). Enzyme activity was expressed as μmol of liberated p-nitrophenol/min/mg of protein.…”

Section: Methodsmentioning

confidence: 99%

Oral administration of β-cryptoxanthin prevents bone loss in ovariectomized rats

Uchiyama

Yamaguchi²

2006

Int J Mol Med

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Abstract. The effect of ß-cryptoxanthin, a kind of carotenoid, on ovariectomy-induced bone loss was investigated. ß-Cryptoxanthin was isolated from Satsuma mandarin (Citrus unshu. MARC). ß-cryptoxanthin (5 or 10 μg/100 g body weight) was orally administered once daily for 3 months to ovariectomized (OVX) rats. OVX induced a significant increase in body weight and a significant decrease in serum calcium and inorganic phosphorus concentrations as compared with those of shamoperated (control) rats. These alterations induced by OVX were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). The analysis using a peripheral quantitative computed tomography (pQCT) showed that OVX induced a significant decrease in mineral content and mineral density in the femoral-diaphyseal and -metaphyseal tissues and polar strength strain index in the metaphyseal tissues. These decreases were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). Moreover, OVX induced a significant decrease in calcium content and alkaline phosphatase activity in the femoraldiaphyseal and -metaphyseal tissues and deoxyribonucleic acid (DNA) content in the metaphyseal tissues. These decreases were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). This study demonstrates that ß-cryptoxanthin has a preventive effect on OVX-induced bone loss in vivo.

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Section: Methodsmentioning

confidence: 99%

Oral administration of β-cryptoxanthin prevents bone loss in ovariectomized rats

Uchiyama

Yamaguchi²

2006

Int J Mol Med

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“…The enzyme assay described below was carried out under optimal conditions. Alkaline phosphatase activity was determined by the method of Walter and Schutt (24). The enzyme activity was expressed as nmol of p-nitrophenol liberated/min/mg of protein.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Genistein and zinc synergistically enhance gene expression and mineralization in osteoblastic MC3T3-E1 cells

Uchiyama

Yamaguchi²

2007

Int J Mol Med

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“…At 2 weeks after operation, they were deeply anesthetized and femurs, tibias, blood and urine were collected for biochemical analysis. Proximal tibia and distal femur were cut out and homogenized in 10 mM Triethanolamin Buffer (pH 7.5), and supernatants were exposed for TRAP activities measured by the method of Walter, 26 and precipitates were hybridized by HCl (6 M) in 1051C for 24 h to measure hydroxyproline on the method by Bergmana, 27 and Ca content were measured based on the OCPC method by Gitelman. 28 Estradiol level in serum was measured by EIA (Mitsubishi Kagaku latron, Tokyo, Japan), and urinary deoxypyridinoline levels were measured on day 14 of the experiments by EIA (Metra Biosystems, Mountain View, CA, USA).…”

Section: Rat Osteoporosis Model By Ovariectomy and Osteogenic Disordementioning

confidence: 99%

NFκB decoy oligodeoxynucleotides ameliorates osteoporosis through inhibition of activation and differentiation of osteoclasts

et al. 2006

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The transcription factor, nuclear factor-kappa B (NFkB), is believed to play a pivotal role in osteoclast formation. In this study, we focused on NFkB decoy oligodeoxynucleotides (ODN) as a new therapeutic strategy to attenuate osteoporosis. Tartrate-resistant acid phosphatase (TRAP)-positive multinuclear osteoclasts formed in mononuclear cells including osteoclast precursors from neonatal rabbit bone marrow were increased in the presence of 1,25-dihydroxyvitamin D3, whereas transfection of NFkB decoy ODN decreased the number of TRAP-positive cells and attenuated RANKL and M-CSF-induced osteoclast formation. NFkB decoy ODN also inhibited the activity of osteoclasts, as assessed by pit formation. In rat overiectomized model of estrogen deficiency, continuous administration of NFkB decoy ODN attenuated the increase of TRAP activity, accompanied by a significant increase in calcium concentration in tibia and femur and decrease in urinary deoxypyridinoline. In additional osteoporosis model using vitamin C-deficient rat, inhibition of NFkB by decoy ODN dramatically improved the bone length, weight, density as assessed by dual-energy X-ray absorptiometry. Overall, inhibition of NFkB by decoy strategy prevented osteoporosis through the inhibition of bone resorption. Targeting of NFkB might be potential therapy in various bone metabolic diseases. Gene Therapy (2006) 13, 933-941.

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Acid and Alkaline Phosphatase in Serum

Cited by 196 publications

References 0 publications

Oral administration of β-cryptoxanthin prevents bone loss in ovariectomized rats

Oral administration of β-cryptoxanthin prevents bone loss in ovariectomized rats

Genistein and zinc synergistically enhance gene expression and mineralization in osteoblastic MC3T3-E1 cells

NFκB decoy oligodeoxynucleotides ameliorates osteoporosis through inhibition of activation and differentiation of osteoclasts

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