Acid Phosphatases of Budding Yeast as a Model of Choice for Transcription Regulation Research

Abstract

Acid phosphatases of budding yeast have been studied for more than forty years. This paper covers biochemical characteristics of acid phosphatases and different aspects in expression regulation of eukaryotic genes, which were researched using acid phosphatases model. A special focus is devoted to cyclin-dependent kinase Pho85p, a negative transcriptional regulator, and its role in maintaining mitochondrial genome stability and to pleiotropic effects of pho85 mutations.

The PHO pathway involved in phosphate metabolism in Yeast for efficient phosphorus removal

“…Phosphate-responsive signal transduction (PHO) pathway, is known to regulate the expression of several phosphate-responsive genes, in order to keep intracellular phosphate concentration stable. In recent years, relevant scholars have continuously studied the PHO pathways of different types of yeast such as Cryptococcus neoformans [12,13], C. glabrata [14], C. albicans [15], and Budding Yeast [16]. Studies pathways [17].…”

“…Studies pathways [17]. Acid phosphatase hydrolyzes extracellular phosphate compounds into inorganic phosphate granules [4], phosphate transporters carry inorganic phosphate granules into the body and store them in the form of polyphosphates as an emergency repository for phosphates [16]; this activity is inhibited under high-Pi conditions.…”

“…After observation by fluorescence microscopy, it was found that yellow fluorescent polyphosphate particles were present in the vacuole. Elena V. Sambuk [16] analyzed gene expression levels in yeast cells on high and low Pi concentrations and found that the genes encoding vacuolar membrane integrin have similar expression levels to many genes in the PHO system and also are adjusted by Pi concentration. Through the determination of phosphate concentration in yeast, it was found that different PHO gene mutant strains have different polyphosphate concentration storage, demonstrating that the concentration of polyphosphate in yeast is regulated by the PHO system.…”

Divergence in expression of PHO3, 5, 11, 12 paralogues yeasts is the mechanism guiding the evolution of multigene families

“…Интерес-но, что репрессирующая концентрация фосфата различ-на у лабораторных штаммов разного происхождения. Синтез рКФ у штаммов Петергофской генетической коллекции подавляется при 250 мг/л неорганического фосфата, тогда как у штаммов GRF18 и S288C ингиби-рование синтеза рКФ происходит только при 1000 мг/л (Sambuk et al, 2011).…”

“…Оптимум рН для Pho3p (3,8), для Pho5p и Pho11p -pH 4,6. Как видно, кКФ более ус-тойчива к стрессорным факторам -закислению среды и повышению температуры (Sambuk et al, 2011).…”