Acid preservation of Saccharina latissima for application as a carbon source for fermentation to biofuels and chemicals

Sandbakken, Ingrid S.; Sæther, Maren; Funderud, Jon; Aasen, Inga Marie

doi:10.1007/s10811-018-1489-z

Cited by 24 publications

(10 citation statements)

References 19 publications

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“…This demonstrates that the seaweeds ensiled under natural conditions underwent a similar fermentation process compared to those provided with a microbial stimulant. This provides further evidence that seaweeds harbor endogenous enzymes required for the hydrolysis of complex carbohydrates, as suggested by Sandbakken et al [41]. L. plantarum is a slow lactic acid producer at pH > 5, and therefore the inability of the pH to drop below 4.9 in FV silage probably limited the effect of the inoculant on silage quality.…”

Section: Discussionsupporting

confidence: 71%

“…The storage carbohydrates in brown seaweeds are mainly composed of laminarin and mannitol, whereas alginate represents the main structural component [12]. Laminarin and mannitol, despite not being included within the WSC fraction [35], represent up to 330 and 190 g/kg DM of SL, respectively [7], and are utilized as a substrate for lactic acid bacteria during the ensiling process [41]. The structural carbohydrates, i.e., alginates, are likely to be more resistant to microbial degradation in the silo [15].…”

Section: Discussionmentioning

confidence: 99%

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The Effect of Ensiling on the Nutritional Composition and Fermentation Characteristics of Brown Seaweeds as a Ruminant Feed Ingredient

Campbell

Ortuño

Ford

et al. 2020

Animals

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Ensiling could be an effective method to preserve seaweeds for animal feed applications, however, there is limited scientific knowledge in this area. Seaweeds are a promising ruminant feed ingredient, in part due to the content of phenolic compounds, which are receiving considerable interest as alternative antimicrobial agents in feed. The aim of the study was to compare the effect of ensiling on the nutritional composition and fermentation characteristics of two brown seaweed species, Fucus vesiculosus (FV) and Saccharina latissimi (SL) with or without the use of a Lactobacillus plantarum (LAB) inoculant. The effect of ensiling on the stability of phlorotannin was also investigated using nuclear magnetic resonance (NMR). After harvesting, the seaweeds were wilted for 24 h and subsequently ensiled in laboratory-scaled silos for 90 days. SL silage showed a stronger fermentation pattern (pH < 4), dominated by lactic acid (50–60 g/kg Dry Matter (DM)), and a slightly higher acetic acid content compared to FV silages (p < 0.05). The fermentability of FV was limited (pH > 4.8) with low lactic acid production (<5 g/kg DM). The addition of the LAB inoculant showed no effect on the fermentation process but a modest effect on the chemical composition of both species was observed after the 90-day ensiling period. The results showed no losses in the nutrient content of FV after ensiling, however losses in the Crude Protein (CP, −32%), ash (−36%), Neutral Detergent Fibre (NDF, −77%) and Acid Detergent Fibre (ADF, −58%) content of SL were observed. The ensiling process had a limited effect on the in vitro true dry matter digestibility and phenolic content of either species. Therefore, ensilage may be a suitable preservation method for the use of brown seaweeds as a ruminant feed; however, species-specific differences were observed.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

The Effect of Ensiling on the Nutritional Composition and Fermentation Characteristics of Brown Seaweeds as a Ruminant Feed Ingredient

Campbell

Ortuño

Ford

et al. 2020

Animals

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“…Frozen, milled S. latissima (two batches, harvested October 2014 and 2016, respectively, from the Trondheim Fjord, Norway) was thawed at 4 • C over night. The extracts (SWEs) were prepared according to the protocol developed by Horn et al (2000a), but using pH ∼3.5, since later works have shown that the laminaran becomes less available for enzymatic hydrolysis after extraction at pH < 3 (Adams et al, 2008;Sandbakken et al, 2018). Briefly, hot water (60-70 • C) was added to the biomass (biomass:water, 1:1, weight basis), and pH was adjusted to ∼3.5 by addition of 1M H 2 SO 4 , before incubation at 70 • C for 1 h to completely dissolve laminaran.…”

Section: Preparation Of Seaweed Extractmentioning

confidence: 99%

“…Laminaran was quantified after acid hydrolysis to glucose (Sandbakken et al, 2018). For determination of mannitol and glucose concentrations in SWEs and culture media, samples were centrifuged and filtered through 0.2 µm syringe filters before HPLC as performed using an Aminex HPX-87-H column (BioRad Laboratories) at 45 • C, and refractive index detection (RID-6A, Shimadzu).…”

Section: Analyses Of Growth Substrates and Productsmentioning

confidence: 99%

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C

et al. 2020

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The facultative methylotroph Bacillus methanolicus MGA3 has previously been genetically engineered to overproduce the amino acids L-lysine and L-glutamate and their derivatives cadaverine and γ-aminobutyric acid (GABA) from methanol at 50 • C. We here explored the potential of utilizing the sugar alcohol mannitol and seaweed extract (SWE) containing mannitol, as alternative feedstocks for production of chemicals by fermentation using B. methanolicus. Extracts of the brown algae Saccharina latissima harvested in the Trondheim Fjord in Norway were prepared and found to contain 12-13 g/l of mannitol, with conductivities corresponding to a salt content of ∼2% NaCl. Initially, 12 B. methanolicus wild type strains were tested for tolerance to various SWE concentrations, and some strains including MGA3 could grow on 50% SWE medium. Non-methylotrophic and methylotrophic growth of B. methanolicus rely on differences in regulation of metabolic pathways, and we compared production titers of GABA and cadaverine under such growth conditions. Shake flask experiments showed that recombinant MGA3 strains could produce similar and higher titers of cadaverine during growth on 50% SWE and mannitol, compared to on methanol. GABA production levels under these conditions were however low compared to growth on methanol. We present the first fed-batch mannitol fermentation of B. methanolicus and production of 6.3 g/l cadaverine. Finally, we constructed a recombinant MGA3 strain synthesizing the C30 terpenoids 4,4 -diaponeurosporene and 4,4 -diapolycopene, experimentally confirming that B. methanolicus has a functional methylerythritol phosphate (MEP) pathway. Together, our results contribute to extending the range of both the feedstocks for growth and products that can be synthesized by B. methanolicus.

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“…A pH of lower than 4.7 is required to prevent the growth of clostridia in Saccorhiza polyschides [76], while below pH 4.48-4.10 is sufficient for S. latissima [58]. Sandbakken et al (2018) [77] found that no sugar loss was detected during 6 months of storage when S. latissima was stored anaerobically at a pH < 4 by the addition of sulphuric and formic acid.…”

Section: Ensilage For Storage and Preservationmentioning

confidence: 99%

A Review of Seaweed Pre-Treatment Methods for Enhanced Biofuel Production by Anaerobic Digestion or Fermentation

et al. 2018

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Macroalgae represent a potential biomass source for the production of bioethanol or biogas. Their use, however, is limited by several factors including, but not restricted to, their continuous supply for processing, and low biofuel yields. This review examines recent pre-treatment processes that have been used to improve the yields of either biogas or bioethanol from macroalgae. Factors that can influence hydrolysis efficiency and, consequently, biofuel yields, are highly affected by macroalgal composition, including content of salts, heavy metals, and polyphenols, structural make-up, as well as polysaccharide composition and relative content of carbohydrates. Other factors that can influence biofuel yield include the method of storage and preservation.

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Acid preservation of Saccharina latissima for application as a carbon source for fermentation to biofuels and chemicals

Cited by 24 publications

References 19 publications

The Effect of Ensiling on the Nutritional Composition and Fermentation Characteristics of Brown Seaweeds as a Ruminant Feed Ingredient

The Effect of Ensiling on the Nutritional Composition and Fermentation Characteristics of Brown Seaweeds as a Ruminant Feed Ingredient

Production of Value-Added Chemicals by Bacillus methanolicus Strains Cultivated on Mannitol and Extracts of Seaweed Saccharina latissima at 50°C

A Review of Seaweed Pre-Treatment Methods for Enhanced Biofuel Production by Anaerobic Digestion or Fermentation

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