2014
DOI: 10.1016/j.ijfoodmicro.2014.03.001
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Acid stress management by Cronobacter sakazakii

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Cited by 46 publications
(18 citation statements)
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“…6b), and the consensus sequence of CpxR site was also found upstream of the fabA and fabB genes in additional bacteria species (Table 1). Additionally, Cronobacter sakazakii clone carrying transposon insertion in cpxR gene was identified as acid-sensitive mutant 49 . All these facts suggest this ATR system functioning in exponential phase is highly conserved across bacteria species.…”
Section: Resultsmentioning
confidence: 99%
“…6b), and the consensus sequence of CpxR site was also found upstream of the fabA and fabB genes in additional bacteria species (Table 1). Additionally, Cronobacter sakazakii clone carrying transposon insertion in cpxR gene was identified as acid-sensitive mutant 49 . All these facts suggest this ATR system functioning in exponential phase is highly conserved across bacteria species.…”
Section: Resultsmentioning
confidence: 99%
“…In this study, 953 clones were obtained by transposition-mediated random mutagenesis, and a single non-motile clone was identified, whose insertion site was mapped within the fliF gene. Several studies, have shown that random mutagenesis by transposition (EZ-Tn5 TM <Kan-2>Tnp Transposome TM ) is an efficient system to generate mutants, allowing the easy identification of their phenotype and mapping [24][25][26][27]. This mutation system has shown a high efficiency ratio in other bacteria, such as E. coli 10 5 > Salmonella enterica serovar Typhimurium 10 4 > Proteus vulgaris 10 3 > Pseudomonas spp.…”
Section: Discussionmentioning
confidence: 99%
“…Also, in Cronobacter , a cross-protection is observed for several stresses. For example, Alvarez-Ordóñez et al (2014) showed that C. sakazakii exposed to acid stress produces activation of several genes encoding chaperone proteins, as DnaJ also involved in heat stress response. Consequently, the way that bacteria undergo environmental perturbation as performed in this study could also promote their survival in gastrointestinal tract until epithelium cells.…”
Section: Discussionmentioning
confidence: 99%