Acidic Glycosaminoglycans in Human Esophagus Tissue

Sekino, Takafumi; Murata, Katsuyuki; Saito, Yasuhiro

doi:10.1620/tjem.127.273

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…By electrophoretic and enzymatic techniques, Sekino and Murata [ 8 ] showed that ChS is a natural constituent of the structure of the esophagus. A protective effect of ChS on an alcohol-induced gastric ulcer in the rat has also been demonstrated [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Rheological Behavior of a New Mucoadhesive Oral Formulation Based on Sodium Chondroitin Sulfate, Xyloglucan and Glycerol

Pecora¹,

Ragazzo²,

Bertin³

et al. 2021

JFB

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Background: The study aimed at assessing the mucoadhesive properties and the barrier effect of a formulation, labelled as AL2106, containing sodium chondroitin sulfate (ChS), xyloglucan from tamarind seed extract, and glycerol, by evaluating the capacity to adhere to a layer of mucin, the rheological synergism and the barrier effect in comparison to the marketed Esoxx One medical device. AL2106 is a medical device distributed by Alfasigma SpA, Italy with REF FTP57 (Manufacturer: Labomar SpA); it is analogous to Esoxx One medical device: the two products are drinkable solutions that, after swallowing, adhere to the esophageal mucosa, protecting it from the corrosive effect of the gastric acid reflux. AL2106 has been conceived to be better performing in terms of duration of the barrier effect compared to Esoxx One. Methods: The mucoadhesive properties, rheological behavior, buffering capacity against acidity, and film-forming ability with the resultant protecting effect on esophagus mucosa (caffeine permeation test) was compared between the two products. Results: The mucoadhesivity of the formulations was shown in vitro: both remained adherent to a mucin layer, also when the support was rotated by 90°, and when the film layer was washed with water, intended to simulate the washout due to swallowing. AL2106 showed a good buffering efficacy, being able to absorb at least 50% of its weight of 0.03 M HCl while maintaining the pH above 4. The film-forming effect and barrier properties of AL2106 and Esoxx One were confirmed by an in vitro study on reconstructed human esophageal epithelium. A greater film-forming efficacy of AL2106, lasting for at least 5 h, than Esoxx One was observed. Noteworthy, the barrier function of esophageal tissues was shown to be preserved after the application of both formulations. Conclusions: The combination of ChS with the mucoadhesive glycerol−xyloglucan complex and other excipients, which contribute to the barrier effect and to mucoadhesion, contained in AL2106, allowed a longer-lasting protective effect than Esoxx One, proving its effectivity and safety for oral use.

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Section: Introductionmentioning

confidence: 99%

Rheological Behavior of a New Mucoadhesive Oral Formulation Based on Sodium Chondroitin Sulfate, Xyloglucan and Glycerol

Pecora¹,

Ragazzo²,

Bertin³

et al. 2021

JFB

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“…Our previous study indicated that acidic glycosaminoglycans (AGAG) of human esophagus comprised mainly hyaluronic acid (HA) (20). Not much information is available on the constituent of esophageal AGAG, though numer ous papers have been reported the compositional and functional changes of AGAG and glycoproteins o f other gastrointestinal tracts in various conditions (5,9,10,16,19).…”

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confidence: 99%

Age-Dependent Constitutional Change of Acidic Glycosaminoglycans in Human Esophagus

Sekino¹,

Murata²

1978

Digestion

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The age-dependent constituents of esophageal acidic glycosaminoglycans (AGAG) were studied using 35 healthy individuals by electrophoretic characterization and enzymic assay with specific chondroitinases and hyaluronidase. Human esophageal AGAG comprised hyaluronic acid, dermatan sulfate, heparan sulfate and chondroitin 4- and 6-sulfate and oversulfated dermatan sulfate. Hyaluronic acid was the major part of AGAG at the young age but it decreased definitely with the advance of age. The proportion of dermatan sulfate and the oversulfated isomer to total AGAG significantly increased until adult age and then tended to somewhat decrease with increasing age. The proportion of heparan sulfate consistently increased with the advance of age.

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