Acidic Xylooligosaccharide Preserves Hepatic Iron Storage Level in Adult Female Rats Fed a Low-Iron Diet

Kobayashi, Yukiko; Ohbuchi, Takayuki; Fukuda, Tomo; Wakasugi, Etsuko; Yasui, Risa; Hamada, Mai; Yokoyama, Meiko; Kuwahata, Masashi; Kido, Yasutoshi

doi:10.3177/jnsv.57.292

Cited by 12 publications

(7 citation statements)

References 19 publications

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“…In this connection, the partial hydrolysis of synthetic soluble fibers (Kobayashi et al, 2011) was shown to increase both iron absorption and serum iron levels when these fibers were fed to rats with iron deficiency anemia. Similarly in the present study, the use of X + C showed that the hydrolysis of native fibers increased iron in vitro bioaccessibility.…”

Section: Discussionmentioning

confidence: 98%

Enzymatic degradation of phytate, polyphenols and dietary fibers in Ethiopian injera flours: Effect on iron bioaccessibility

Baye

Guyot²,

Icard‐Vernière³

et al. 2015

Food Chemistry

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Section: Discussionmentioning

confidence: 98%

Enzymatic degradation of phytate, polyphenols and dietary fibers in Ethiopian injera flours: Effect on iron bioaccessibility

Baye

Guyot²,

Icard‐Vernière³

et al. 2015

Food Chemistry

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“…5 Hardwood U-XOS with an average degree of polymerization 8-10 as diet was examined in adult female rats, showing the prevention of iron deficiency anemia. 6 Hardwood U-XOS has also served as a precursor for the formation of sulfated U-XOS designated as pentosan polysulfate, providing polysaccharides with the biological properties of glycosaminoglycans. Formulations of these products have provided orally administered treatments for interstitial cystitis (painful bladder disease) in humans 7 and injections for treatments of osteoarthritis in horses.…”

Section: Introductionmentioning

confidence: 99%

Production of acidic xylooligosaccharides from methylglucuronoarabinoxylans by Bacillus subtilis strain MR44

Rhee

Preston

et al. 2015

J of Chemical Tech & Biotech

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BACKGROUND Bacillus subtilis 168 secretes endoxylanases Xyn11A and Xyn30C and arabinofuranohydrolase Axh43 that process xylans in hemicellulose fractions. Engineered strain MR44 with a deletion in the xynA gene encoding Xyn11A, converts hardwood methylglucuronoxylans (MeGXn) of dicots to defined acidic xylooligosaccharides (U‐XOS) with a single methylglucuronate linked to xylooligosaccharides with an average ratio of one methylglucuronate to 6–7 xyloses as value‐added products. The MR44 strain also secretes Axh43 that catalyses release of arabinose from methylglucuronoarabinoxylans (MeGAXn) of monocots. RESULTS MR44 strain efficiently processes sorghum MeGAXn with 493 mg g−1 yields of purified U‐XOS. 1H‐NMR analysis of U‐XOS purified by anion exchange chromatography structurally defined the U‐XOS with an average degree of polymerization of xylose units (DP) of 11–12 and a single 4‐O‐methyl‐glucuronic acid α‐1,2‐linked on average to one of 11–12 xylose residues each penultimate to the reducing terminal xylose. CONCLUSION This study demonstrates the ability of Bacillus subtilis strains to process MeGAXn for production of U‐XOS from monocots, including energy crops and agricultural residues. The U‐XOS with an average DP of 11–12 from sweet sorghum MeGAXn compared with 6–7 from sweetgum MeGXn extends their applications as biologicals and for production of pentosan polysulfates with applications in human and veterinary medicine. © 2015 Society of Chemical Industry

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“…Furthermore, acidic XOSs are of interest as potential pharmaceutical agents due to their immunomodulating effects, prevention of iron deficiency anemia, and protection against the development of atopic dermatitis, etc. (3,(7)(8)(9).…”

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confidence: 99%

GH30-7 Endoxylanase C from the Filamentous Fungus Talaromyces cellulolyticus

Nakamichi

Fujii

Fouquet³

et al. 2019

Appl Environ Microbiol

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Glycoside hydrolase family 30 subfamily 7 (GH30-7) enzymes include various types of xylanases, such as glucuronoxylanase, endoxylanase, xylobiohydrolase, and reducing-end xylose-releasing exoxylanase. Here, we characterized the mode of action and gene expression of the GH30-7 endoxylanase from the cellulolytic fungus Talaromyces cellulolyticus (TcXyn30C). TcXyn30C has a modular structure consisting of a GH30-7 catalytic domain and a C-terminal cellulose binding module 1, whose cellulose-binding ability has been confirmed. Sequence alignment of GH30-7 xylanases exhibited that TcXyn30C has a conserved Phe residue at the position corresponding to a conserved Arg residue in GH30-7 glucuronoxylanases, which is required for the recognition of the 4-O-methyl-α-d-glucuronic acid (MeGlcA) substituent. TcXyn30C degraded both glucuronoxylan and arabinoxylan with similar kinetic constants and mainly produced linear xylooligosaccharides (XOSs) with 2 to 3 degrees of polymerization, in an endo manner. Notably, the hydrolysis of glucuronoxylan caused an accumulation of 22-(MeGlcA)-xylobiose (U4m2X). The production of this acidic XOS is likely to proceed via multistep reactions by putative glucuronoxylanase activity that produces 22-(MeGlcA)-XOSs (XnU4m2X, n ≥ 0) in the initial stages of the hydrolysis and by specific release of U4m2X from a mixture containing XnU4m2X. Our results suggest that the unique endoxylanase activity of TcXyn30C may be applicable to the production of linear and acidic XOSs. The gene xyn30C was located adjacent to the putative GH62 arabinofuranosidase gene (abf62C) in the T. cellulolyticus genome. The expression of both genes was induced by cellulose. The results suggest that TcXyn30C may be involved in xylan removal in the hydrolysis of lignocellulose by the T. cellulolyticus cellulolytic system. IMPORTANCE Xylooligosaccharides (XOSs), which are composed of xylose units with a β-1,4 linkage, have recently gained interest as prebiotics in the food and feed industry. Apart from linear XOSs, branched XOSs decorated with a substituent such as methyl glucuronic acid and arabinose also have potential applications. Endoxylanase is a promising tool in producing XOSs from xylan. The structural variety of XOSs generated depends on the substrate specificity of the enzyme as well as the distribution of the substituents in xylan. Thus, the exploration of endoxylanases with novel specificities is expected to be useful in the provision of a series of XOSs. In this study, the endoxylanase TcXyn30C from Talaromyces cellulolyticus was characterized as a unique glycoside hydrolase belonging to the family GH30-7, which specifically releases 22-(4-O-methyl-α-d-glucuronosyl)-xylobiose from hardwood xylan. This study provides new insights into the production of linear and branched XOSs by GH30-7 endoxylanase.

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Acidic Xylooligosaccharide Preserves Hepatic Iron Storage Level in Adult Female Rats Fed a Low-Iron Diet

Cited by 12 publications

References 19 publications

Enzymatic degradation of phytate, polyphenols and dietary fibers in Ethiopian injera flours: Effect on iron bioaccessibility

Enzymatic degradation of phytate, polyphenols and dietary fibers in Ethiopian injera flours: Effect on iron bioaccessibility

Production of acidic xylooligosaccharides from methylglucuronoarabinoxylans by Bacillus subtilis strain MR44

GH30-7 Endoxylanase C from the Filamentous Fungus Talaromyces cellulolyticus

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