Acidiferrimicrobium australe gen. nov., sp. nov., an acidophilic and obligately heterotrophic, member of the Actinobacteria that catalyses dissimilatory oxido-reduction of iron isolated from metal-rich acidic water in Chile

González, Daniella; Huber, Katharina J.; Tindall, Brian J.; Hedrich, Sabrina; Rojas-Villalobos, Camila; Quatrini, Raquel; Dinamarca, M. Alejandro; Ibacache-Quiroga, Claudia; Schwarz, Alex; Valenzuela, Carla V.; Nancucheo, Iván

doi:10.1099/ijsem.0.004179

Cited by 19 publications

(8 citation statements)

References 32 publications

(44 reference statements)

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“…Enzymatic and non-enzymatic antioxidants mechanisms involved in protection against oxidative stress ( Imlay, 2019 ) are thus deemed essential for adaptation and survival in these environments. A variety of these antioxidant systems have been described in Acidithiobacillus and in other iron oxidizers ( Ferrer et al, 2016b ; Bellenberg et al, 2019 ; Feng et al, 2020 ; González et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

et al. 2022

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The bioleaching process is carried out by aerobic acidophilic iron-oxidizing bacteria that are mainly mesophilic or moderately thermophilic. However, many mining sites are located in areas where the mean temperature is lower than the optimal growth temperature of these microorganisms. In this work, we report the obtaining and characterization of two psychrotolerant bioleaching bacterial strains from low-temperature sites that included an abandoned mine site in Chilean Patagonia (PG05) and an acid rock drainage in Marian Cove, King George Island in Antarctic (MC2.2). The PG05 and MC2.2 strains showed significant iron-oxidation activity and grew optimally at 20°C. Genome sequence analyses showed chromosomes of 2.76 and 2.84 Mbp for PG05 and MC2.2, respectively, and an average nucleotide identity estimation indicated that both strains clustered with the acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans. The Patagonian PG05 strain had a high content of genes coding for tolerance to metals such as lead, zinc, and copper. Concordantly, electron microscopy revealed the intracellular presence of polyphosphate-like granules, likely involved in tolerance to metals and other stress conditions. The Antarctic MC2.2 strain showed a high dosage of genes for mercury resistance and low temperature adaptation. This report of cold-adapted cultures of the At. ferrooxidans species opens novel perspectives to satisfy the current challenges of the metal bioleaching industry.

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Section: Resultsmentioning

confidence: 99%

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

et al. 2022

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“…majanohamensis and R. sediminis [5, 7–14]. To date, only a few studies have briefly described the potential ecological functions of the acidimicrobial species by metagenomic analysis [15].…”

Section: Full-textmentioning

confidence: 99%

“…The genus Rhabdothermincola was first proposed as a new genus within this family by Liu et al with Rhabdothermincola sediminis SYSU G02662 T as the type species isolated from hot spring sediments [7]. However, due to the existing difficulties and challenges in isolating Acidimicrobiia members under normal culture conditions, so far only 10 species of this class have been successfully isolated, cultured and validly published, namely Desertimonas flava, Ilumatobacter fluminis, Acidiferrimicrobium australe, Acidimicrobium ferrooxidans, Ferrimicrobium acidiphilum, Ferrithrix thermotolerans, Actinomarinicola tropica, Aquihabitans daechungensis, I. majanohamensis and R. sediminis [5,[7][8][9][10][11][12][13][14]. To date, only a few studies have briefly described the potential ecological functions of the acidimicrobial species by metagenomic analysis [15].…”

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confidence: 99%

Rhabdothermincola salaria sp. nov., a novel actinobacterium isolated from a saline lake sediment

Gao

Fang

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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An actinobacterium, designated strain EGI L10124T, was isolated from saline lake sediment collected in Xinjiang province, PR China. The taxonomic position of the isolate was determined based on polyphasic taxonomic and phylogenomic analyses. Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that strain EGI L10124T formed a distinct clade with Rhabdothermincola sediminis SYSU G02662T, with a shared sequence identity of 95.2 %. The novel isolate could be distinguished from species in the genus Rhabdothermincola by its distinct phenotypic, physiological and genotypic characteristics. The cells of strain EGI L10124T were aerobic, Gram-stain-positive and short rod-shaped. Optimal growth conditions of strain EGI L10124T on marine agar 2216 were registered at pH 8.0 at 37 °C. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major respiratory quinone was MK-9 (H8), while the major fatty acids were iso-C16 : 0, C17 : 0 and C16 : 0. The polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. Based on the genome sequence of strain EGI L10124T, it appears that the G+C content of the novel isolate was 71.8 mol%. According to our data, strain EGI L10124T represents a new species of the genus Rhabdothermincola , for which the name Rhabdothermincola salaria sp. nov. is proposed. The type strain of the proposed novel isolate is EGI L10124T (=CGMCC 1.19113T=KCTC 49679T).

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“…Most currently known iron-reducing heterotrophic acidophiles found in pyritic mine tailings are Proteobacteria [32]. Interestingly, dissimilatory reduction of ferric iron has also been described for other genera of acidophilic actinobacteria, including Ferrimicrobium acidiphilum, Ferrithrix thermotolerans, Aciditerrimonas ferrireducens and Acidithrix ferrooxidans [33], and the novel recently-described genus Acidiferrimicrobium australe [11]. In addition, species of acidophilic actinobacteria, except Aciditerrimonas ferrireducens and the isolate IT2, also oxidize ferrous iron.…”

Section: Metal Mic (Mm)mentioning

confidence: 99%

“…), which use the ferrous iron and/or reduced sulfur as electron donors. Most species of iron-oxidizing acidophiles that oxidize iron when oxygen is present can also use ferric iron as an alternative electron acceptor in anaerobic environments [11]. Some species of obligately heterotrophic acidophiles, including Acidiphilium, Acidocella and Acidobacterium which are able to reduce ferric iron but not oxidize ferrous iron, have also been identified in mine tailings [8].…”

Section: Introductionmentioning

confidence: 99%

Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits

Nancucheo

Johnson

2020

Microorganisms

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Reactive pyritic mine tailings can be populated by chemolithotrophic prokaryotes that enhance the solubilities of many metals, though iron-reducing heterotrophic microorganisms can inhibit the environmental risk posed by tailings by promoting processes that are the reverse of those carried out by pyrite-oxidising autotrophic bacteria. A strain (IT2) of Curtobacterium ammoniigenes, a bacterium not previously identified as being associated with acidic mine wastes, was isolated from pyritic mine tailings and partially characterized. Strain IT2 was able to reduce ferric iron under anaerobic conditions, but was not found to catalyse the oxidation of ferrous iron or elemental (zero-valent) sulfur, and was an obligate heterotrophic. It metabolized monosaccharides and required small amounts of yeast extract for growth. Isolate IT2 is a mesophilic bacterium, with a temperature growth optimum of 30 °C and is moderately acidophilic, growing optimally at pH 4.0 and between pH 2.7 and 5.0. The isolate tolerated elevated concentrations of many transition metals, and was able to grow in the cell-free spent medium of the acidophilic autotroph Acidithiobacillus ferrooxidans, supporting the hypothesis that it can proliferate in acidic mine tailings. Its potential role in mitigating the production of acidic, metal-rich drainage waters from mine wastes is discussed.

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Acidiferrimicrobium australe gen. nov., sp. nov., an acidophilic and obligately heterotrophic, member of the Actinobacteria that catalyses dissimilatory oxido-reduction of iron isolated from metal-rich acidic water in Chile

Abstract: A novel acidophilic member of the phylum Actinobacteria was isolated from an acidic, metal-contaminated stream draining from an abandoned underground coal mine (Trongol mi… Show more

Cited by 19 publications

References 32 publications

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

Characterization and genomic analysis of two novel psychrotolerant Acidithiobacillus ferrooxidans strains from polar and subpolar environments

Rhabdothermincola salaria sp. nov., a novel actinobacterium isolated from a saline lake sediment

Characteristics of an Iron-Reducing, Moderately Acidophilic Actinobacterium Isolated from Pyritic Mine Waste, and Its Potential Role in Mitigating Mineral Dissolution in Mineral Tailings Deposits

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