Acidophilic microbial communities associated with a natural, biodegraded hydrocarbon seepage

Röling, Wilfred F. M.; Ortega-Lucach, S.; Larter, Stephen R.; Head, Ian M.

doi:10.1111/j.1365-2672.2006.02926.x

Cited by 36 publications

(16 citation statements)

References 41 publications

(62 reference statements)

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“…and Mycobacterium spp. are known to degrade or to be associated with the degradation of hydrocarbons in low pH environments (Dore et al 2003;Röling et al 2006;Uyttebroek et al 2007;López et al 2008). The results from this study suggested that the microbial community associated with the Sydney Tar Ponds sediment was dominated by heterotrophic acidophiles and potential acidophilic hydrocarbon degraders.…”

Section: Discussionmentioning

confidence: 58%

Characterization of the bacterial community structure of Sydney Tar Ponds sediment

et al. 2011

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The Sydney Tar Ponds is one of the largest toxic waste sites in Canada. The bacterial diversity and abundance in the Sydney Tar Ponds sediment was examined using a 16S rRNA gene clone library and denaturing gradient gel electrophoresis (DGGE) with four different primer sets. The clone library was grouped into 19 phylotypes that could be divided into five phyla: Proteobacteria (56.9%), Actinobacteria (35%), Acidobacteria (4.9%), Firmicutes (2.4%), and Verrucomicrobia (0.8%). Members of the phyla Actinobacteria (represented mainly by Mycobacterium spp.) and Alphaproteobacteria (represented by Acidocella spp.) comprised the majority of the clone library. This study also revealed that the phylogenetic results obtained from clone library analysis and from DGGE analysis, with all the primer sets, showed some variability. However, similar Mycobacterium spp. and Acidocella spp. were found in all the different DGGE analyses, again suggesting that these two genera are dominant in the Sydney Tar Ponds sediment. In addition, DGGE analysis indicated that primer sets targeting the V3 region produced results that were the most similar to those obtained with the clone library.

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Section: Discussionmentioning

confidence: 58%

Characterization of the bacterial community structure of Sydney Tar Ponds sediment

et al. 2011

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“…Burkholderiaceae and Acetobacteraceae prevailed in lower pH, whereas Pseudomonadaeceae and Sphingomonadaceae were shown to prefer higher pH. Several genera belonging to Acetobacteraceae have been linked with hydrocarbon degradation at moderately low pH levels (Hamamura et al, 2005;Rö ling et al, 2006). Sphingomonadaceae, Burkholderiaceae and Pseudomonadaceae are recognized for their extraordinary catabolic flexibility and are among the most common hydrocarbon degraders in a variety of soils (Leys et al, 2004;Stolz, 2009;Silby et al, 2011;Pérez-Pantoja et al, 2012); however, a thorough knowledge on pH optima of these groups is still lacking.…”

Section: Discussionmentioning

confidence: 99%

Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site

et al. 2014

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Restoration of polluted sites via in situ bioremediation relies heavily on the indigenous microbes and their activities. Spatial heterogeneity of microbial populations, contaminants and soil chemical parameters on such sites is a major hurdle in optimizing and implementing an appropriate bioremediation regime. We performed a grid-based sampling of an aged creosote-contaminated site followed by geostatistical modelling to illustrate the spatial patterns of microbial diversity and activity and to relate these patterns to the distribution of pollutants. Spatial distribution of bacterial groups unveiled patterns of niche differentiation regulated by patchy distribution of pollutants and an east-to-west pH gradient at the studied site. Proteobacteria clearly dominated in the hot spots of creosote pollution, whereas the abundance of Actinobacteria, TM7 and Planctomycetes was considerably reduced from the hot spots. The pH preferences of proteobacterial groups dominating in pollution could be recognized by examining the order and family-level responses. Acidobacterial classes came across as generalists in hydrocarbon pollution whose spatial distribution seemed to be regulated solely by the pH gradient. Although the community evenness decreased in the heavily polluted zones, basal respiration and fluorescein diacetate hydrolysis rates were higher, indicating the adaptation of specific indigenous microbial populations to hydrocarbon pollution. Combining the information from the kriged maps of microbial and soil chemistry data provided a comprehensive understanding of the long-term impacts of creosote pollution on the subsurface microbial communities. This study also highlighted the prospect of interpreting taxa-specific spatial patterns and applying them as indicators or proxies for monitoring polluted sites.

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“…Organisms related to G. ferruginea were not detected in the neutral/alkaline stream. Sequence analysis of DNA cloned from the acidic stream revealed that presumed acidophilic bacteria related to genera such as Acidocella, Acidiphilium, and Acidobacterium (8,31,32,37) were prevalent and comprised 7% of the total population of clones. Analysis of clones obtained from the very acidic stream revealed large representation by a few eukaryotic species (64% of clones), which were not deliberately targeted using the universal bacterial primers.…”

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confidence: 99%

Biofilm Bacterial Community Structure in Streams Affected by Acid Mine Drainage

Lear

Niyogi

Harding

et al. 2009

Appl Environ Microbiol

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We examined the bacterial communities of epilithic biofilms in 17 streams which represented a gradient ranging from relatively pristine streams to streams highly impacted by acid mine drainage (AMD). A combination of automated ribosomal intergenic spacer analysis with multivariate analysis and ordination provided a sensitive, high-throughput method to monitor the impact of AMD on stream bacterial communities. Significant differences in community structure were detected among neutral to alkaline (pH 6.7 to 8.3), acidic (pH 3.9 to 5.7), and very acidic (pH 2.8 to 3.5) streams. DNA sequence analysis revealed that the acidic streams were generally dominated by bacteria related to the iron-oxidizing genus Gallionella, while the organisms in very acidic streams were less diverse and included a high proportion of acidophilic eukaryotes, including taxa related to the algal genera Navicula and Klebsormidium. Despite the presence of high concentrations of dissolved metals (e.g., Al and Zn) and deposits of iron hydroxide in some of the streams studied, pH was the most important determinant of the observed differences in bacterial community variability. These findings confirm that any restoration activities in such systems must focus on dealing with pH as the first priority.

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Acidophilic microbial communities associated with a natural, biodegraded hydrocarbon seepage

Cited by 36 publications

References 41 publications

Characterization of the bacterial community structure of Sydney Tar Ponds sediment

Characterization of the bacterial community structure of Sydney Tar Ponds sediment

Spatial patterns of microbial diversity and activity in an aged creosote-contaminated site

Biofilm Bacterial Community Structure in Streams Affected by Acid Mine Drainage

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