acj6: a gene affecting olfactory physiology and behavior in Drosophila.

Ayer, Richard K.; Carlson, John R.

doi:10.1073/pnas.88.12.5467

Cited by 62 publications

(37 citation statements)

References 28 publications

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“…6 ) . They are also consistent with the results of Ayer and Carlson ( 1991 ). which showed an effect ofacj6 on response to ethyl acetate, although the shape of the dose dependence, which was recorded from a different position on the antennal surface, was somewhat different from that shown here.…”

Section: Eag Amplitude Is Dependent On Recording Electrode Locationsupporting

confidence: 94%

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

1992

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This article provides characterization of the electrical response to odorants in the Drosophila antenna and provides physiological evidence that a second organ, the maxillary palp, also has olfactory function in Drosophila. The acj6 mutation, previously isolated by virtue of defective olfactory behavior, affects olfactory physiology in the maxillary palp as well as in the antenna. Interestingly, abnormal chemosensory jump 6 (acj6) reduces response in the maxillary palp to all odorants tested except benzaldehyde (odor of almond), as if response to benzaldehyde is mediated through a different type of odorant pathway from the other odorants. In other experiments, different parts of the antenna are shown to differ with respect to odorant sensitivity. Evidence is also provided that antennal response to odorants varies with age, and that odorants differ in their age dependence.

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Section: Eag Amplitude Is Dependent On Recording Electrode Locationsupporting

confidence: 94%

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

1992

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“…2E), significantly innervated the contralateral AL, and connected the ALs with various neuropils in addition to the MB and LH of the same side (see below). Furthermore, although the POU domain transcription factor Acj6 (Ayer and Carlson, 1991) is still expressed in every adPN and not detectable in the vPNs, despite the inclusion of GH146-negative progenies, cells that express Acj6 near PNs of the lateral lineage (Komiyama et al, 2003) clearly belong to the lAL lineage (Fig. 2C,G-I).…”

Section: Resultsmentioning

confidence: 99%

Clonal analysis ofDrosophilaantennal lobe neurons: diverse neuronal architectures in the lateral neuroblast lineage

et al. 2008

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The antennal lobe (AL) is the primary structure in the Drosophila brain that relays odor information from the antennae to higher brain centers. The characterization of uniglomerular projection neurons (PNs) and some local interneurons has facilitated our understanding of olfaction; however, many other AL neurons remain unidentified. Because neuron types are mostly specified by lineage and temporal origins, we use the MARCM techniques with a set of enhancer-trap GAL4 lines to perform systematical lineage analysis to characterize neuron morphologies, lineage origin and birth timing in the three AL neuron lineages that contain GAL4-GH146-positive PNs: anterodorsal, lateral and ventral lineages. The results show that the anterodorsal lineage is composed of pure uniglomerular PNs that project through the inner antennocerebral tract. The ventral lineage produces uniglomerular and multiglomerular PNs that project through the middle antennocerebral tract. The lateral lineage generates multiple types of neurons, including uniglomeurlar PNs, diverse atypical PNs, various types of AL local interneurons and the neurons that make no connection within the ALs. Specific neuron types in all three lineages are produced in specific time windows, although multiple neuron types in the lateral lineage are made simultaneously. These systematic cell lineage analyses have not only filled gaps in the olfactory map, but have also exemplified additional strategies used in the brain to increase neuronal diversity.

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“…Electrophysiological recordings were performed as described previously (Ayer and Carlson, 1991). Two-to 6-d-old female flies were immobilized on ice and wedged into an appropriately trimmed tapering ends of a yellow pipette tip allowing the head to protrude.…”

Section: Methodsmentioning

confidence: 99%

Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

Kain¹,

Chakraborty²,

Sundaram³

et al. 2008

J. Neurosci.

110

117

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Mechanisms by which G-protein-coupled odorant receptors transduce information in insects still need elucidation. We show that mutations in the Drosophila gene for G q ␣ (dgq) significantly reduce both the amplitude of the field potentials recorded from the whole antenna in responses to odorants as well as the frequency of evoked responses of individual sensory neurons. This requirement for G q ␣ is for adult function and not during antennal development. Conversely, brief expression of a dominant-active form of G q ␣ in adults leads to enhanced odor responses. To understand signaling downstream of G q ␣ in olfactory sensory neurons, genetic interactions of dgq were tested with mutants in genes known to affect phospholipid signaling. dgq mutant phenotypes were further enhanced by mutants in a PLC␤ (phospholipase C␤) gene, plc21C. Interestingly although, the olfactory phenotype of mutant alleles of diacylglycerol kinase (rdgA) was rescued by dgq mutant alleles. Our results suggest that G q ␣-mediated olfactory transduction in Drosophila requires a phospholipid second messenger the levels of which are regulated by a cycle of phosphatidylinositol 1,4-bisphosphate breakdown and regeneration.

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acj6: a gene affecting olfactory physiology and behavior in Drosophila.

Cited by 62 publications

References 28 publications

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

Clonal analysis ofDrosophilaantennal lobe neurons: diverse neuronal architectures in the lateral neuroblast lineage

Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

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acj6: a gene affecting olfactory physiology and behavior in Drosophila.

Cited by 62 publications

References 28 publications

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 Distinguishes two classes of odorant pathways

Clonal analysis ofDrosophilaantennal lobe neurons: diverse neuronal architectures in the lateral neuroblast lineage

Reduced Odor Responses from Antennal Neurons of Gqα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

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Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction