Reduced Odor Responses from Antennal Neurons of Gqα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

Kain, Pinky; Chakraborty, Tuhin S.; Sundaram, Susinder; Siddiqi, Obaid; Rodrigues, Veronica; Hasan, Gaiti

doi:10.1523/jneurosci.5306-07.2008

Cited by 110 publications

(125 citation statements)

References 50 publications

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“…In contrast, another study indicates an additional metabotropic response requiring the recruitment of G-proteinmediated second-messenger pathways that is delayed and slower relative to the initial ionotropic response (Wicher et al 2008). Although the question remains open, the incorporation of G-protein dependent pathways that presumably require the synthesis of second-messenger intermediates is also consistent with numerous studies linking ORs to GPCR signalling pathways (Kain et al 2008, Kalidas and Smith 2002, Woodard et al 1992.…”

Section: Mosquito Olfactory Transduction: a Road Map Under Constructionsupporting

confidence: 59%

“…The initial and very fast response is cAMP-independent, while a second and much more sensitive response is G s -mediated and cAMP-dependent. More or less simultaneously Kain et al (2008) showed very strong evidence for a G q and a phospholipase C involvement. The transduction system is not clearly understood, and contains a number of different transduction pathways.…”

Section: Transduction Mechanismsmentioning

confidence: 95%

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Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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Section: Mosquito Olfactory Transduction: a Road Map Under Constructionsupporting

confidence: 59%

Section: Transduction Mechanismsmentioning

confidence: 95%

Olfaction in vector-host interactions

Takken¹,

Knols²

2010

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“…In insects, both metabotropic [16][17][18] and inotropic [19][20] mode of signaling have been proposed to mediate olfactory transduction at ORNs. Until recently, insect ORs were considered to be a member of GPCRs [21][22]; however, new evidence suggests that insect ORs exhibit a different membrane topology and differ in OR protein sequence from vertebrate ORs [23][24].…”

Section: Short-communicationmentioning

confidence: 99%

New Insights into the Roles of cAMP and InsP3 on Odor-Processing and Olfactory-Driven Behaviors

Murmu¹

2017

J Cell Signal

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The olfactory system is one of our fundamental senses, responsible for the detection of airborne chemicals from our surroundings. These chemicals include small volatile molecules, proteins, peptides to gaseous substances, such as carbon dioxide, and provide valuable information regarding the location of food, mates and potential danger. The ability of the olfactory system to detect and orchestrate appropriate behavioral response to these chemicals is critical for the organism's survival. Given the vast array of chemical substances in the environment, it seems inevitable that the olfactory system uses a large repertoire of odorant receptors (ORs), signaling pathways and olfactory subsystems to sample its surroundings.Over recent decades, the molecular and cellular mechanism underlying odor signal transduction has been studied in great detail and is well established. We now know that in vertebrates and C. Elegans, odorants interact primarily with ORs, which are G-protein coupled receptor (GPCRs) located on the membrane of olfactory receptor neurons (ORNs). Binding of an odor to an OR triggers the activation of second messenger cascades, of which, adenosine 3', 5'-cyclic monophosphate (cAMP) was one of the first second messengers proposed to mediate olfactory transduction at ORNs. Experiments using biochemical and molecular biological techniques provided evidence that when an odor binds to an OR, it stimulates the rapid synthesis of cAMP via the activation of GPCRs and adenyl cyclase [1][2]. Increased levels of cAMP cause the opening of cyclic-nucleotidegated channels (CNGC) [3][4], which are highly permeable to Ca 2+ [5] and their opening enhances Ca 2+ concentration within the ORNs [6][7]. cAMP-mediated Ca 2+ increase has been shown to activate a second conductance, such as Cl-or K + [8][9], which ultimately results in the generation of action potentials that carry odor-information to the first-order olfactory centre (e.g. olfactory bulb) in the brain.Studies carried out in the last decade also indicate that cAMP is not the only second messenger mediating olfactory transduction at ORNs. There is considerable evidence indicating that some odorants do not induce an increase in cAMP but rather the concentration of inositol, 1, 4, 5-triphosphate (InsP3) is increased [1,10]. InsP3 formation can increase intracellular Ca 2+ [11] and activate Ca 2+ -dependent K + channels or Ca 2+ -dependent non-specific cation channels [1,[12][13], which in turn generate receptor potential and depolarize the olfactory neuron, thus providing a signal for further neuronal processing. In addition to cAMP and InsP3, diverse olfactory transduction pathways, such as the activation of cAMP/cGMP-gated channels [14] reported a fast ionotrophic response and found no evidence of the involvement of G-protein or cAMP, cGMP or InsP3 while Wicher et al. [20] showed that insect ORs induce the synthesis of cAMP through a G-protein that in turn activates Or83b, which serves as a cAMP-gated ion channel.It is clear that there are fundamental differences ...

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“…Surprisingly, though, both the insect gustatory and olfactory receptors act as ligand-gated cation channels when expressed in heterologous cell culture systems (Sato et al, 2008;Wicher et al, 2008). Perhaps even more surpris-ingly, several studies have reported conflicting results using different expression systems and experimental approaches and concluded that at least some members of the superfamily couple to G proteins (Kain et al, 2008;Wicher et al, 2008).…”

Section: Olfactory Co 2 Detection Mechanismsmentioning

confidence: 99%

Olfactory Carbon Dioxide Detection by Insects and Other Animals

Jones

2013

Molecules and Cells

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Carbon dioxide is a small, relatively inert, but highly volatile gas that not only gives beer its bubbles, but that also acts as one of the primary driving forces of anthropogenic climate change. While beer brewers experiment with the effects of CO 2 on flavor and climate scientists are concerned with global changes to ambient CO 2 levels that take place over the course of decades, many animal species are keenly aware of changes in CO 2 concentration that occur much more rapidly and on a much more local scale. Although imperceptible to us, these small changes in CO 2 concentration can indicate imminent danger, signal overcrowding, and point the way to food. Here I review several of these CO 2 -evoked behaviors and compare the systems insects, nematodes, and vertebrates use to detect environmental CO 2 .

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Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

Cited by 110 publications

References 50 publications

Olfaction in vector-host interactions

Olfaction in vector-host interactions

New Insights into the Roles of cAMP and InsP3 on Odor-Processing and Olfactory-Driven Behaviors

Olfactory Carbon Dioxide Detection by Insects and Other Animals

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