Acoustic and hybrid 3D-printed electrochemical biosensors for the real-time immunodetection of liver cancer cells (HepG2)

Damiati, Samar; Küpcü, Seta; Peacock, M. A.; Eilenberger, Christoph; Zamzami, Mazin A.; Qadri, Ishtiaq; Choudhry, Hani; Sleytr, Uwe B.; Schuster, Bernhard

doi:10.1016/j.bios.2017.03.045

Cited by 71 publications

(44 citation statements)

References 55 publications

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“…Although there are several challenges, given the considerable potential applications, it is worthwhile to devote more efforts to this promising multifunctional platform for drug delivery and biomedical applications. In recent years, Slp has been gradually employed to construct novel biosensors 155 for cancer diagnosis, 156 detection of chemical ions, 157 and nanoelectronic applications. 158 We believe this is indeed a signal of the future biomedical development of Slp-coated liposomes.…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Luo¹,

Yang²,

Yao³

et al. 2019

IJN

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Slp forms a crystalline array of proteins on the outermost envelope of bacteria and archaea with a molecular weight of 40-200 kDa. Slp can self-assemble on the surface of liposomes in a proper environment via electrostatic interactions, which could be employed to functionalize liposomes by forming Slp-coated liposomes for various applications. Among the molecular characteristics, the stability, adhesion, and immobilization of biomacromolecules are regarded as the most meaningful. Compared to plain liposomes, Slp-coated liposomes show excellent physicochemical and biological stabilities. Recently, Slp-coated liposomes were shown to specifically adhere to the gastrointestinal tract, which was attributed to the "ligand-receptor interaction" effect. Furthermore, Slp as a "bridge" can immobilize functional biomacromolecules on the surface of liposomes via protein fusion technology or intermolecular forces, endowing liposomes with beneficial functions. In view of these favorable features, Slp-coated liposomes are highly likely to be an ideal platform for drug delivery and biomedical uses. This review aims to provide a general framework for the structure and characteristics of Slp and the interactions between Slp and liposomes, to highlight the unique properties and drug delivery as well as the biomedical applications of the Slp-coated liposomes, and to discuss the ongoing challenges and perspectives.

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Section: Challenges and Perspectivesmentioning

confidence: 99%

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Luo¹,

Yang²,

Yao³

et al. 2019

IJN

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show abstract

“…EIS can be used to investigate the properties of a material or specific processes that can affect the capacitivity or conductivity/resistivity of an electrochemical system [2,4,68]. EIS technique is widely used in electrochemical biosensors based on S-layer proteins for several applications to assess membrane-active peptide insertion and membrane protein reconstitution into artificial lipid membranes and specific cell detection [69][70][71][72][73].…”

Section: Impedimetric Biosensorsmentioning

confidence: 99%

“…Natural and synthetic membranes are selective to permeable ions that cross between the inside and outside of membranes. Further, high electrochemical potentials can be maintained across the interior and exterior monolayers [72,73,77,78]. The chemical nature of the membrane components and the reactions that occur at the interface of the monolayer or within the bilayer govern the permeability and structural properties of the membrane.…”

Section: Sensor Surface Modificationsmentioning

confidence: 99%

“…The protein A binding site of rSbpA/ZZ helps to immobilize the antibodies (IgG) in the correct orientation [113]. Thus, rSbpA/ZZ, when used to generate a uniform matrix on a gold electrode, enables the immobilization of anti-CD133 antibodies in the correct orientation to enhance the recognition and capture of the surface marker CD133, which is expressed on membranes of hepatic cancer stem cells [72]. The CV technique allowed evaluation of the functionality of the developed sensor.…”

Section: Biosensor For Sensing Cellsmentioning

confidence: 99%

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Electrochemical Biosensors Based on S-Layer Proteins

Damiati

Schuster

2020

Sensors

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Designing and development of electrochemical biosensors enable molecule sensing and quantification of biochemical compositions with multitudinous benefits such as monitoring, detection, and feedback for medical and biotechnological applications. Integrating bioinspired materials and electrochemical techniques promote specific, rapid, sensitive, and inexpensive biosensing platforms for (e.g., point-of-care testing). The selection of biomaterials to decorate a biosensor surface is a critical issue as it strongly affects selectivity and sensitivity. In this context, smart biomaterials with the intrinsic self-assemble capability like bacterial surface (S-) layer proteins are of paramount importance. Indeed, by forming a crystalline two-dimensional protein lattice on many sensors surfaces and interfaces, the S-layer lattice constitutes an immobilization matrix for small biomolecules and lipid membranes and a patterning structure with unsurpassed spatial distribution for sensing elements and bioreceptors. This review aims to highlight on exploiting S-layer proteins in biosensor technology for various applications ranging from detection of metal ions over small organic compounds to cells. Furthermore, enzymes immobilized on the S-layer proteins allow specific detection of several vital biomolecules. The special features of the S-layer protein lattice as part of the sensor architecture enhances surface functionalization and thus may feature an innovative class of electrochemical biosensors.

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“…Biosensors are bioanalytical devices integrating biorecognition elements and suitable transducers, which could be further designed with various signal amplification strategies to obtain extremely high sensitivity [ 2 ]. With plenty of advantages, such as strong specificity to targets, rapid analysis, high accuracy, easy operation, and low cost, biosensors have expansive applications for diverse targets, including cyclic drugs [ 20 ], proteins [ 21 , 22 , 23 ], nucleic acids [ 24 , 25 , 26 , 27 ], and cyclic tumor cells [ 28 , 29 , 30 ]. Immunosensors have played a crucial role in the determination of tumor markers [ 31 , 32 , 33 ], significantly improving early diagnosis and prognosis of various cancers.…”

Section: Introductionmentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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Acoustic and hybrid 3D-printed electrochemical biosensors for the real-time immunodetection of liver cancer cells (HepG2)

Cited by 71 publications

References 55 publications

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

<p>Slp-coated liposomes for drug delivery and biomedical applications: potential and challenges</p>

Electrochemical Biosensors Based on S-Layer Proteins

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

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