Here, we introduce Traffic Ear, an acoustic sensor pack that determines the engine noise of each passing vehicle without interrupting traffic flow. The device consists of an array of microphones combined with a computer vision camera. The class and speed of passing vehicles were estimated using sound wave analysis, image processing, and machine learning algorithms. We compared the traffic composition estimated with the Traffic Ear sensor with that recorded using an automatic number plate recognition (ANPR) camera and found a high level of agreement between the two approaches for determining the vehicle type and fuel, with uncertainties of 1–4%. We also developed a new bottom-up assessment approach that used the noise analysis provided by the Traffic Ear sensor along with the extensively detailed urban mobility maps that were produced using the geospatial and temporal mapping of urban mobility (GeoSTMUM) approach. It was applied to vehicles travelling on roads in the West Midlands region of the UK. The results showed that the reduction in traffic engine noise over the whole of the study road was over 8% during rush hours, while the weekday–weekend effect had a deterioration effect of almost half. Traffic noise factors (dB/m) on a per-vehicle basis were almost always higher on motorways compared the other roads studied.