Non-destructive techniques have always been used in the study of built cultural heritage because of the high cultural value of the concerned objects and the need to preserve them as intact as possible. In this chapter, different non-destructive techniques applied to the conservation of historical building are presented. The selected techniques concern the measurement of some physical properties of the building materials measured at the surface: water absorption, permeability, water content, cohesion, hardness and so on; the actual conditions of the building: stress state, deformation, crack growth and so on; and in-depth physical properties: mechanical properties, inner structure of walls, damp location and salt content. Some of these techniques are used for inspection of the building at a given time, whereas others can be applied for long periods of time to investigate the evolution of the building or of one of its parts (e.g., crack propagation) with time. After presenting the physical background of each method, the main objective of this review is to focus on the applications, especially to discuss which information can be supplied and to present published results in each case. Some techniques are very simple and require very inexpensive equipment but others, which are mainly adaptations of field geophysical techniques, use more sophisticated technology and require post-acquisition treatments based on more complex physical principles. Finally, some examples of combinations of different techniques are presented because a unique method cannot provide all the information needed to understand the weathering processes taking place in a building. The final goal of the studies is to contribute to the preservation of the cultural heritage. The choice of the conservation strategies and methods should be based on a deep knowledge of the building, and nondestructive testing is usually the only way to get it.