Acquisition of Beta-Lactamase by Neisseria meningitidis through Possible Horizontal Gene Transfer

Hong, Eva; Deghmane, Ala‐Eddine; Taha, Muhamed-Kheir

doi:10.1128/aac.00831-18

Cited by 22 publications

(10 citation statements)

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“…In contrast, the Italian isolate 2816 was found to lack the insert, and hence lacked the bla ROB-1 gene, and was also found to have 241 and 238 gene allele differences from NMLY144 and M17 240005, respectively. During revision of our manuscript, a serogroup Y invasive N. meningitidis strain resistant to penicillin due to acquisition of the bla ROB-1 gene has been reported in France 48 . The origin of the bla ROB-1 gene in this strain was also suggested to be the H. influenzae plasmid pB1000 because 100% identity was found between the bla ROB-1 genes in these two organisms.…”

Section: Discussionmentioning

confidence: 85%

WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene

Tsang

Ahmad

Tyrrell

2018

Journal of Antimicrobial Chemotherapy

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Objectives Neisseria meningitidis is rarely penicillin resistant. We describe WGS analysis of a penicillin-resistant N. meningitidis collected from a case of invasive meningococcal disease.MethodsSerogrouping, serotyping and serosubtyping were performed with specific antibodies. β-Lactamase was detected by nitrocefin. MICs were determined by Etest and agar dilution. Sequencing of N. meningitidis genomes was done on the Illumina MiSeq platform and genome data were analysed using the Bacterial Isolate Genome Sequence Database (BIGSdb) on the PubMLST Neisseria website (https://pubmlst.org/neisseria/). Transformation was used to confirm the genetic basis of the penicillin resistance.ResultsAn N. meningitidis blood isolate from a female patient in her mid-50s with a painful and septic left shoulder was found to have penicillin MIC values of 3–12 mg/L. The isolate was typed as Y: 14, 19: P1.– and ST3587, and was weakly β-lactamase positive. WGS analysis identified a full-length copy of the β-lactamase gene blaROB-1, which was contained on a 1719 bp insert with a G + C content of 41.7% (versus a G + C content of N. meningitidis of 51.7%), suggesting that the blaROB-1 gene came from a different bacterial species. A GenBank analysis of the blaROB-1 gene insert found 99.77% identity with a DNA segment found in plasmid pB1000′ from Haemophilus influenzae. Transformation of a penicillin-susceptible strain with the blaROB-1 gene conferred β-lactamase activity and penicillin resistance.Conclusions N. meningitidis serogroup Y, ST3587 can carry and express the blaROB-1 gene, leading to penicillin resistance. It is highly likely that the N. meningitidis isolate acquired the blaROB-1 gene from H. influenzae.

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Section: Discussionmentioning

confidence: 85%

WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene

Tsang

Ahmad

Tyrrell

2018

Journal of Antimicrobial Chemotherapy

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“…It is well documented, however, that invasive N. meningitidis species are becoming more resistant to such treatments. 53 Resistance to this specific class of antibiotics is rarely due to beta-lactamase producing meningococci. 54 More frequently in N. meningitides , mutations in penicillin-binding proteins within bacterial species may also limit the effectiveness of penicillin and are the leading cause of reduced susceptibility among meningoccoci.…”

Section: Antibiotic Resistancementioning

confidence: 99%

Meningococcal disease surveillance in the Asia–Pacific region (2020): The global meningococcal initiative

Aye

Bai

Borrow

et al. 2020

Journal of Infection

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The incidence of IMD is low and similar across the represented countries (< 0.2 cases per 10 0,0 0 0 persons per year), with the predominant serogroups of Neisseria meningitidis being B, W and Y, although serogroups A and X are present in some areas. Resistance to ciprofloxacin is also of concern, with the close monitoring of antibiotic-resistant clonal complexes (e.g., cc4821) being a priority. Meningococcal vaccination is only included in a few National Immunization Programs, but is recommended for high-risk groups, including travellers (such as pilgrims) and people with complement deficiencies or human immunodeficiency virus (HIV). Both polysaccharide and conjugate vaccines form part of recommendations. However, cost and misconceptions remain limiting factors in vaccine uptake, despite conjugate vaccines preventing the acquisition of carriage.

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“…A 2-day course of rifampin or a single injection of ceftriaxone are recommended alternatives to ciprofloxacin for prophylaxis of contacts of persons with meningococcal disease (4) but are logistically more challenging to administer. A single dose of azithromycin can also be used for prophylaxis in communities where ciprofloxacin-resistant meningococci have been detected; however, there is only a (6) and France (10). The potential circulation of penicillin-resistant or ciprofloxacin-resistant meningococci in other countries merits further investigation.…”

Section: Discussionmentioning

confidence: 99%

Detection of Ciprofloxacin-Resistant, β-Lactamase–Producing Neisseria meningitidis Serogroup Y Isolates — United States, 2019–2020

McNamara¹,

Cubeñas-Potts²,

Blain³

et al. 2020

MMWR Morb. Mortal. Wkly. Rep.

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Acquisition of Beta-Lactamase by Neisseria meningitidis through Possible Horizontal Gene Transfer

Cited by 22 publications

References 9 publications

WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene

WGS analysis of a penicillin-resistant Neisseria meningitidis strain containing a chromosomal ROB-1 β-lactamase gene

Meningococcal disease surveillance in the Asia–Pacific region (2020): The global meningococcal initiative

Detection of Ciprofloxacin-Resistant, β-Lactamase–Producing Neisseria meningitidis Serogroup Y Isolates — United States, 2019–2020

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