Acquisition of Group A streptococcal M protein antibodies

Quinn, Robert W.; Zwaag, Roger Vander; Lowry, P. N.

doi:10.1097/00006454-198507000-00008

Cited by 28 publications

(20 citation statements)

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“…23 We have shown that both teenage and adult Aboriginal and PNG subjects living in highly endemic areas for GAS have significantly higher levels of serum antibodies to the conserved region epitope than children, particularly those aged up to 4 years. These observations are comparable to previous studies which found that adult sera have strong antibody responses to the conserved region of the M6 protein.…”

Section: Discussionmentioning

confidence: 84%

Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci

et al. 1996

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SUMMARYThis study demonstrates the presence of epitope-specific opsonic human antibodies in a population living in an area endemic for group A streptococci (GAS) infection. Antibodies recognizing a conserved C-terminal region epitope (p145, sequence in single letter amino acids: LRRDLDASREAKKQVE-KALE) of the M protein of GAS were isolated from human patients by affinity chromatography and were shown to be of the immunoglobulin G1 (IgG1) and IgG3 subclasses. These antibodies could reduce the number of colonies of serotype 5 GAS in an in vitro opsonization assay by 71-92%, compared with an equal amount of IgG from control adult donors living in non-endemic areas and without antibodies to p145. Addition of the peptide, p145, completely inhibited this opsonization. Indirect immunofluorescence showed that p145-specific antibodies were capable of binding to the surface of M5 GAS whereas control IgG did not. Using chimeric peptides, which contain overlapping segments of p145, each 12 amino acids in length, inserted into a known helical peptide derived from the DNA binding protein of yeast, GCN4, we have been able to further define two minimal regions within p145, referred to as pJ2 and pJ7. These peptides, pJ2 and pJ7, were able to inhibit opsonization by p145 specific antibodies. Finally, we have observed an association between the age-related development of immunity to GAS and the acquisition of antibodies to the conserved epitope, p145, raising the possibility of using this epitope as a target in a prophylactic vaccine administered during early childhood.

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Section: Discussionmentioning

confidence: 84%

Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci

et al. 1996

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“…All four of the M types used in this study have previously been described in the pharyngeal carrier state [8,10]. A classic definition of GAS colonization is an asymptomatic individual with a positive throat culture and no serologic response.…”

Section: Discussionmentioning

confidence: 99%

“…A classic definition of GAS colonization is an asymptomatic individual with a positive throat culture and no serologic response. However, some evidence suggests that there may be some acquisition of type-specific antibody after colonization, but that this does not afford long-term protection against homologous GAS carriage [2,3,10,13].…”

Section: Discussionmentioning

confidence: 99%

Age-associated differences in prevalence of group A streptococcal type-specific M antibodies in children

Jaggi

Dale

Chiang³

et al. 2008

Eur J Pediatr

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Our prior studies of the molecular epidemiology of group A streptococcus (GAS) pharyngitis indicated that the most common emm types associated with pediatric pharyngitis in North America were 12, 1, 28, and 4. We previously reported that the proportions of pediatric pharyngitis due to emm types 12 and 4 decreased with increasing age throughout childhood. We hypothesized that this is due to age-associated acquisition of antibodies to the amino-terminal type-specific region of common GAS M proteins during childhood. We sought to demonstrate this in sera from healthy children by using ELISAs for M 12, 1, 28, and 4. Enzyme-linked immunosorbent assays (ELISA) using chemically synthesized peptides copying amino-terminal type-specific regions of the M proteins were performed on sera from four age groups of healthy children (group I: 3-6 years, group II: 7-10 years, group III: 11-14 years, group IV: 15-18 years). ELISA data were correlated with opsonophagocytic assays for a subset of sera and M 1 GAS. Sera from healthy 12-20-month-old children were used as negative controls. Our results showed that the highest percentage of positivity was for M12, which also showed progressive seropositivity in older children. For the other serotypes, the highest seroprevalence rates were in the 11-14-year-old age group. The presence of ELISA antibodies against M1 correlated with opsonophagocytic activity, a previously studied indicator of immunologic protection.

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“…12 In the endemic areas, it has been well established that children up to 10 years old have high incidence rates of streptococcal skin and respiratory infections, which decreases rapidly with increasing age. 35 A Figure 3. Comparative analysis of the serum antibody concentration against J14.1 and J14-R6 peptides in an agestratified North Indian population.…”

Section: Discussionmentioning

confidence: 99%

Immune response against M protein-conserved region peptides from prevalent group A Streptococcus in a North Indian population

Gupta¹,

Sekhar

Dhanda³

et al. 2016

Journal of Microbiology, Immunology and Infection

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Acquisition of Group A streptococcal M protein antibodies

Cited by 28 publications

References 0 publications

Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci

Opsonic human antibodies from an endemic population specific for a conserved epitope on the M protein of group A streptococci

Age-associated differences in prevalence of group A streptococcal type-specific M antibodies in children

Immune response against M protein-conserved region peptides from prevalent group A Streptococcus in a North Indian population

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