Acquisition of susceptibility to Vibrio penaeicida in Penaeus stylirostris postlarvae and juveniles

Goarant, Cyrille; Brizard, Raphaël; Marteau, Anne-Laure

doi:10.1016/s0044-8486(98)00380-9

Cited by 20 publications

(16 citation statements)

References 6 publications

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“…However, most Vibrio species are now widely accepted as being opportunistic and may cause severe mortalities in larvae or juveniles, which suffer from stress or any deficiency. V. penaeicida disease is unique since this bacteria has been shown to be predominantly pathogenic for juvenile and adult shrimps, whereas post-larvae stages remain unaffected [16,27]. Indeed, vibrioses are mainly known to affect shrimp larvae and post-larvae.…”

Section: Discussionmentioning

confidence: 99%

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

Muñoz

Vandenbulcke

Garnier

et al. 2004

Cellular and Molecular Life Sciences (CMLS)

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The present study reports for the first time the involvement of an antimicrobial peptide in the defense reactions of a shrimp infected by a pathogenic Vibrio, Vibrio penaeicida. New members of the penaeidin family were characterized in the shrimp Litopenaeus stylirostris by RT-PCR and RACE-PCR from hemocyte total RNAs, and by mass spectrometry detection and immunolocalization of mature peptides in shrimp hemocytes. In infected shrimps, bacteria and penaeidin distribution colocalized in the gills and the lymphoid organ that represented the main infected sites. Moreover, the shrimp immune response to infection involved massive hemocyte recruitment to infection sites where released penaeidin may participate in the isolation and elimination of the bacteria, We show that the ability of the shrimps to circumvent shrimp infections is closely related to a recovery phase based on the hematopoietic process.

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Section: Discussionmentioning

confidence: 99%

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

Muñoz

Vandenbulcke

Garnier

et al. 2004

Cellular and Molecular Life Sciences (CMLS)

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“…Mortality was tabulated after 7 d. These preliminary experiments showed that the following dosages resulted in killing approximately half of the population in 7 d: injections of 10 4 and 10 6 CFU V. parahaemolyticus and V. harveyi, respectively, and immersion in ASW containing 10 5 CFU ml -1 of either species. These dosages were selected for use in the present study and are also similar to published lethal dose (LD 50 ) values on shrimp, although different species, different lengths of exposure, and different stages in shrimp life cycles were used (see Arume 1989, Chen et al 1992, Jiravanichpaisal & Miyazaki 1994, Hameed 1995, Lee et al 1996, Goarant et al 1998, Vandenberghe et al 1999, Mikulski et al 2000.…”

Section: Methodsmentioning

confidence: 99%

Vibrio parahaemolyticus and V. harveyi cause detachment of the epithelium from the midgut trunk of the penaeid shrimp Sicyonia ingentis

Martin

Rubin²,

Swanson³

2004

Dis. Aquat. Org.

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Shrimp Sicyonia ingentis were either injected with Vibrio parahaemolyticus (10 4 CFU) or V. harveyi (10 6 CFU) or immersed in ASW containing either species at 10 5 CFU ml -1. These densities were shown in preliminary experiments to kill approximately half the population by 7 d. On Day 7, surviving shrimp were classified as either diseased or apparently healthy, and their midgut trunks (MGT) were examined by light and electron microscopy. All shrimp immersed in ASW containing either species of Vibrio showed detachment of the epithelium in the MGT. In shrimp injected with either species of Vibrio, epithelial detachment was common in diseased shrimp but not in apparently healthy animals. Experiments with live shrimp were supported by in vitro experiments where MGTs were removed, tied off at both ends, and injected with either pathogenic bacteria (V. parahaemolyticus or V. harveyi), non-pathogenic bacteria (Bacillus subtilis or Escherichia coli), or ASW. After 2 h incubations in ASW at 15°C, the MGTs were processed and examined. The epithelium consistently detached from isolated MGTs injected with either species of Vibrio, but not from MGTs injected with non-pathogenic bacteria or ASW. Because the MGT epithelium secretes the peritrophic membrane, loss of the epithelium eliminates 2 layers that may restrict penetration of ingested pathogens into the shrimp body and may disrupt the osmoregulatory function of the MGT. A second finding was that fixed, large-granule hemocytes associated with the basal lamina degranulated in the presence of the 2 species of Vibrio, but not with the non-pathogenic bacteria or ASW. These blood cells may help fight specific bacteria penetrating the MGT. KEY WORDS: Shrimp · Vibriosis · Midgut trunk · HemocytesResale or republication not permitted without written consent of the publisher

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“…Experimental infections of aquatic animals using viruses, bacteria or parasites can be performed to fulfil different objectives: to estimate the efficiency of preventive treatments, study routes of infection, test the resistance of selected lines (Gomez-Leon et al, 2008;Goyard et al, 2008;Lallias et al, 2008) or different ploidy groups (Ching et al, 2009;O'Flynn et al, 1997), and to assess host factors linked to susceptibility (Goarant et al, 1998(Goarant et al, , 2006bLabreuche et al, 2006a). For bacteria, experimental infections are also often used to explore pathogenicity (Gay et al, 2004;Goarant et al, 2006b), coupled with phenotypic and/or molecular characterization of virulence factors.…”

Section: Potential and Limitations Of Bath Challenges Vs Injectionmentioning

confidence: 99%

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

Decker

Normand

Saulnier

et al. 2011

Journal of Invertebrate Pathology

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Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohistochemical analyses showed the affinity of Vibrio for gonadic tissues, highlighting the importance of sexual maturity for vibriosis infection processes in oysters. Mortality rate results showed poor repeatability between tanks, however, in this bath challenge. We then tested a standardized and repeatable injection protocol using two different doses of the same combination of two Vibrio species on related diploid and triploid oysters at four different times over a year. Statistical analyses of mortality kinetics over a 6-day period after injection revealed that active gametogenesis periods correspond to higher susceptibility to vibriosis and that there is a significant interaction of this seasonal effect with ploidy. However, no significant advantage of triploidy was observed. Triploid oysters even showed lower survival than diploid counterparts in winter. Results are discussed in relation to differing energy allocation patterns between diploid and triploid Pacific oysters.

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Acquisition of susceptibility to Vibrio penaeicida in Penaeus stylirostris postlarvae and juveniles

Cited by 20 publications

References 6 publications

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

Involvement of penaeidins in defense reactions of the shrimp Litopenaeus stylirostris to a pathogenic vibrio

Vibrio parahaemolyticus and V. harveyi cause detachment of the epithelium from the midgut trunk of the penaeid shrimp Sicyonia ingentis

Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status

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