Acquisition of thermotolerance and HSP gene expression in durum wheat (Triticum durum Desf.) cultivars

Rampino, Patrizia; Mita, Giovanni; Pataleo, Stefano; Pascali, Mariarosaria De; Fonzo, N. di; Perrotta, Carla

doi:10.1016/j.envexpbot.2009.04.001

Cited by 45 publications

(40 citation statements)

References 24 publications

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“…Expression of gene of HSPs in durum wheat cultivars and acquisition of thermo-tolerance was determined by Rampino et al [115]. Plants are vulnerable to heat stress and they tend to overcome the vulnerability by modifying their several physiological and biochemical mechanisms.…”

Section: Effect Of Heat Stress On Wheat Biochemistrymentioning

confidence: 99%

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Impacts of Heat Stress on Wheat: A Critical Review

Iqbal¹,

Raja²,

Yasmeen³

et al. 2017

Adv Crop Sci Tech

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A brief review is given of investigations on stress-induced alterations in the yield of different biochemical contents in wheat. Wheat is major cereal crop for fulfilling the calories demands of growing population. Alterations in the worldwide climate are predicted to have critical sentence for crop production. Abiotic stresses such as heat and drought are major abiotic stresses restraining crop production. Heat stress reduces wheat growth by upsetting various physiological and biochemical processes and the developmental stage of the plant is critical in demonstrating the vulnerability of various species and cultivars subjected to high temperature. Heat stress did not affect the protein content but there is strong correlation between leaf nitrogen content and grain protein content. Induction of HSPs seems to be the universal response and adaptation to temperature stress. The synthesis of HSPs is believed to play significant role either in preventing or minimizing the adverse effects of high temperature both at molecular and cellular levels. Wheat has the tendency to adopt diverse types of responses to temperature stress as well as a heat shock by developing thermo-tolerance for the enhancement of the grain quality and yield.

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Section: Effect Of Heat Stress On Wheat Biochemistrymentioning

confidence: 99%

“…cultivars was studied by Rampinoa et al [115]. At the cellular and molecular levels, the synthesis of HSPs is essential in preventing or minimizing the toxic effect of high temperature.…”

Section: Thermo-tolerance In Wheatmentioning

confidence: 99%

Impacts of Heat Stress on Wheat: A Critical Review

Iqbal¹,

Raja²,

Yasmeen³

et al. 2017

Adv Crop Sci Tech

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“…Prior studies (Rampino et al, 2009;Huther et al, 2013) have found that the HSP23.6 gene is typically expressed in response to various pressures, and its expression levels are strongly up-regulated during some developmental stages (Sarkar et al, 2009). Slight increase in HSP23.6 gene transcription was reported in tomato fruits under hypoxia (Pegoraro et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

et al. 2017

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Soil flooding is an environmental stressor for crops that can affect physiological performance and reduce crop yields. Abiotic stressors cause changes in protein synthesis, modifying the levels of a series of proteins, especially the heat shock proteins (HSP), and these proteins can help protect the plants against abiotic stress. The objective of this study was to verify if tomato plants cv. Micro-Tom from different genotypes with varying expression levels of MT-sHSP23.6 (mitochondrial small heat shock proteins) have different responses physiological to flooding. Plants from three genotypes (untransformed, MT-sHSP23.6 sense expression levels and MT-sHSP23.6 antisense expression levels) were cultivated under controlled conditions. After 50 days, the plants were flooded for 14 days. After this period half of the plants from each genotype were allowed to recover. Chlorophyll fluorescence, gas exchange, chlorophyll index, leaf area and dry matter were evaluated. Flood stress affected the photosynthetic electron transport chain, which is related to inactivation of the oxygen-evolving complex, loss of connectivity among units in photosystem II, oxidation-reduction of the plastoquinone pool and activity of photosystem I. The genotype with MT-sHSP23.6 sense expression levels was less sensitive to stress from flooding.Keywords: Solanum lycopersicum Mill, photosynthesis, chlorophyll fluorescence, stress.Efeitos do estresse por alagamento em plantas de tomateiro 'Micro-Tom' transformada com diferentes níveis de sHSP23.6 mitocondrial ResumoO alagamento do solo é um estressor ambiental para as culturas e pode afetar o desempenho fisiológico e reduzir a produtividade das culturas. Estresses abióticos causam mudanças na síntese de proteínas, modificando os níveis de uma série de proteínas, em especial as proteínas de choque térmico (HSP) e essas proteínas são conhecidas por proteger as plantas contra estresses abióticos. O objetivo deste estudo foi verificar se as plantas do tomateiro cv. Micro-Tom de distintos genótipos com diferentes níveis de expressão da MT-sHSP23.6 (proteínas mitocondriais de choque térmico com pequena massa molecular), têm diferentes respostas fisiológicas ao alagamento. As plantas de três genótipos (não-transformado, transformado com orientação antisense e transformado com orientação sense para MT-sHSP23.6) foram cultivadas sob condições controladas. Após 50 dias as plantas foram alagadas durante 14 dias. Após esse período as plantas de cada genótipo foram recuperadas. Foram avaliados fluorescência da clorofila, trocas gasosas, índice de clorofila, área foliar e massa seca. O estresse por alagamento afetou a cadeia de transporte de elétrons da fotossíntese, que está relacionado à inativação do complexo de evolução do oxigênio, perda da conectividade entre as unidades do fotossistema II, de oxidação e redução do pool de plastoquinona e atividade do fotossistema I. O genótipo com orientação sense MT-sHSP23.6 foi menos sensível ao estresse por alagamento.Palavras-chave: Solanum lycopersicum Mill, f...

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“…Plants overcome high-temperature stress by several physiological and biochemical mechanisms (Rampino et al 2009). These include morphology or short-term avoidance or acclimation mechanisms involving leaf orientation, transpiration cooling or alterations of membrane lipid composition (Wahid 2007).…”

Section: Introductionmentioning

confidence: 99%

“…These include morphology or short-term avoidance or acclimation mechanisms involving leaf orientation, transpiration cooling or alterations of membrane lipid composition (Wahid 2007). Plant responses to high temperatures are mediated by both their inherent ability to survive (basal tolerance), and their ability to acquire tolerance to otherwise lethal temperatures (acclimation) (Rampino et al 2009). These two mechanisms in cereals are due to the activation of different genetic systems (Maestri et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

Selvaraj

Burow²,

Burke³

et al. 2011

Plant Growth Regul

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Heat can be one of the major abiotic stresses that adversely affect crop production worldwide at different stages of development. As field screening for heat tolerance can be inconsistent and seasonally-limited, it is important to develop a reliable protocol under controlled conditions that allows simultaneous screening of multiple genotypes. The objective of this research was to develop a straightforward laboratory protocol using acquired thermotolerance (ATT) in peanut seedlings as a measure of one mechanism of heat stress tolerance. Sixteen genotypes, including selected accessions of the US peanut minicore collection along with standard checks, were evaluated for acquired themotolerance in two independent experiments. A change in the temperature sensitivity of chlorophyll accumulation was used as an indicator of acquired thermotolerance. Pre-incubation at 38°C for 4 h before the 30-min 50°C challenge triggered the acquired thermotolerance system of the leaf disks, resulting in chlorophyll accumulation upon exposure to light. There was considerable variation among genotypes for ATT in both experiments. Genotypic ranking for mean ATT values were highly correlated (0.949) in both experiments. The effect of seed weight on ATT was not significant. This method is relatively simple and inexpensive and can be used to screen a large number of genotypes.

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Acquisition of thermotolerance and HSP gene expression in durum wheat (Triticum durum Desf.) cultivars

Cited by 45 publications

References 24 publications

Impacts of Heat Stress on Wheat: A Critical Review

Impacts of Heat Stress on Wheat: A Critical Review

Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

Heat stress screening of peanut (Arachis hypogaea L.) seedlings for acquired thermotolerance

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