Acridine orange for diagnosis of malaria – Our experience

Abstract

Light microscopy of Romanowsky stained peripheral blood smear examination is the age old and commonest method for laboratory diagnosis of malaria. However it is labor intensive, time consuming and requires qualified personnel. So fluorescent microscopy variation that is Quantitative Buffy Coat method( QBC) came into vogue. But QBC imposes cost limitation despite its high performance. Therefore we decided to evaluate fluorescent method using acridine orange in our set up. In this study we have compared Acridine Orange staining with Leishman’s staining as gold standard. The sensitivity and specificity of acridine orange was 99.28% and 97.19% respectively, while positive predictive value (PPV) and negative predicitive value was 89.93% and 99.82% respectively.South East Asia Journal of Public Health Vol.6(1) 2016: 49-51

A preliminary Study of Malaria infection (Plasmodium spp.) in Iraqi Livestock

“…The fluorescence microscopy (BX51 Olympus U-RFL-T-Japan) was used to look and enumeration for the malaria parasites by mixing a drop of infected blood with an acridine orange solution (0.1 mg/mL). Then, thin smears were made and examined under 100X [26]. The sizes of infected erythrocytes were measured relative to surrounding uninfected erythrocytes.…”

Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites

“…None of the present molecular techniques can detect all mixed infections together, but microscopic examination offers chances for this to be made in Giemsa stained blood smears.However, the process of fixing, drying and staining of the blood smears in addition to their examination are ratherlaboriousandrequire at least 60 minutes from specimen collection to the report of the result [9]. The diagnosis can be a difficulty, especially when parasitaemia are low or blood smear preparation is less than optimal [10]. To enhance and easediagnosis of hemoparasites, several methods are presented for the quick diagnosis of hemoparasites.…”

“…Motile parasitic such as Trypanosoma spp. and microfilaria can be screened by QBC [11,15].It iswidely used for Trypanosoma and microfilariae detections in humans [10] and dogs [15,16]. Also, a similar method was used to identify Babesia spp.…”