Acridine orange–poly (α‐<scp>L</scp>‐glutamic acid) complexes. I. Stoichiometry and stacking coefficients

Myhr, Brian Cecil; Foss, John G.

doi:10.1002/bip.360100302

Cited by 28 publications

(6 citation statements)

References 14 publications

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“…Titration data were analyzed in the same manner as those reported elsewhere. [5][6][7]9 At an early stage in the titration, the spectra have a predominant peak at 492 nm. This peak corresponds to the absorption of three species: free monomer, free dimer, and bound dye.…”

Section: Resultsmentioning

confidence: 99%

Dye binding and its relation to polyelectrolyte conformation

Tan¹,

Schneider²

1975

J. Phys. Chem.

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lod'ko, and A. N. Faidsh, Opt. Spektrosk., 22, 232 (1967). (13) Errors represent 95% confidence limits throughout.(14) The photostationary state isomer ratio is the same in systems wlth benzophenone adsorbed on silica gel' or with benzaldehyde in the gas phase15 as that observed with benzophenone in solution.e This may mean that kl/k2 is the same in all these systems. It should be noted that the composition of the photostatlonary state reported here (56.5%) Is close to that (58.5%) reported for the other cases, but the agreement arises because here an increase in kllk2 compensates for a decrease in kl/kc (cf. eq V). (15) Unpublished work by M . 4 . Fontaine, quoted in G. R. DeMare, P. Goldfinger, G. Huybrechts, E. Jonas, and M. Toth, Ber. Bunsenges, Phys. Chem., 73, 867 (1969). Publication costs assisted by Eastman Kodak CompanyPolymer-dye interaction and its relation to the chain conformation of the hydrophobic polyelectrolyte, sodium copoly(ethy1 acrylate-acrylic acid) (mole ratio 3:l) have been investigated by measuring the binding of a fluorescent dye, 6-ptoluidinonaphthalene-2-sulfonate (TNS), and a cationic dye, Acridine Orange (AO), to this copolymer. Data on the relative fluorescence intensity of TNS, spectrophotometric titration, sedimentation, and dialysis of the AO-polymer system suggest that dye binding is a sensitive function of polymer chain conformation. A change in the conformation of this copolymer, from an extended coil at low ionic strength to a compact coil at high ionic strength, has been found to occur in the region from 0.1 to 0.5 M NaCl.

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Section: Resultsmentioning

confidence: 99%

Dye binding and its relation to polyelectrolyte conformation

Tan¹,

Schneider²

1975

J. Phys. Chem.

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“…These hydrophobic interactions might explain why these two monovalent CPCs bind the GAG in contrast to monovalent arginine. In support, it has been found that soluble acridine orange AOR can self-assemble when interacting with anionic polyelectrolytes or interfaces (33,43,44). It may be noted that the release of solvent water also plays a key role in binding and aggregation of polycations with other polyelectrolytes such as DNA (45)(46)(47).…”

Section: Binding Mechanismmentioning

confidence: 96%

Binding and Clustering of Glycosaminoglycans: A Common Property of Mono- and Multivalent Cell-Penetrating Compounds

Ziegler

Seelig

2008

Biophysical Journal

115

109

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Recent observations in cell culture provide evidence that negatively charged glycosaminoglycans (GAGs) at the surface of biological cells bind cationic cell-penetrating compounds (CPCs) and cluster during CPC binding, thereby contributing to their endocytotic uptake. The GAG binding and clustering occur in the low-micromolar concentration range and suggest a tight interaction between GAGs and CPCs, although the relation between binding affinity and specificity of this interaction remains to be investigated. We therefore measured the GAG binding and clustering of various mono- and multivalent CPCs such as DNA transfection vectors (polyethylenimine; 1,2-dioleoyl-3-trimethylammonium-propane), amino acid homopolymers (oligoarginine; oligolysine), and cell-penetrating peptides (Penetratin; HIV-1 Tat) by means of isothermal titration calorimetry and dynamic light scattering. We find that these structurally diverse CPCs share the property of GAG binding and clustering. The binding is very tight (microscopic dissociation constants between 0.34 and 1.34 microM) and thus biologically relevant. The hydrodynamic radius of the resulting aggregates ranges from 78 nm to 586 nm, suggesting that they consist of numerous GAG chains cross-linked by CPCs. Likewise, the membrane-permeant monovalent cation acridine orange leads to GAG binding and clustering, in contrast to its membrane-impermeant structural analogs propidium iodide and ethidium bromide. Because the binding and clustering of GAGs were found to be a common denominator of all CPCs tested, these properties might be helpful to identify further CPCs.

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“…Equations 12 and 13 easily reduce to the case of second neighbor (or first neighbor) interaction by setting qg = 1 (or qa = q2 = 1). The fraction of sequences of length m is25 Fm = Eiq3m-392m-2(9iu)m-1 (14) The fraction of molecules in the mth aggregate state is Xm = rnFm , (15) where, of course…”

Section: Mathematical Formalismmentioning

confidence: 99%

Interaction between Acridine Orange and poly(styrenesulfonic acid)

Vitagliano¹,

Costantino²,

Zagari³

1973

J. Phys. Chem.

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The interaction between Acridine Orange and poly(styrenesulfonic acid) has been studied spectrophotometrically. The experimental results can be interpreted by an "Ising-type" model which accounts for the adsorption of dye molecules along an infinite linear chain of fixed adsorption sites. The effect of temperature and added salt on the Acridine Orange-poly(styrenesulfonic acid) has been also investigated.

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Acridine orange–poly (α‐L‐glutamic acid) complexes. I. Stoichiometry and stacking coefficients

Cited by 28 publications

References 14 publications

Dye binding and its relation to polyelectrolyte conformation

Dye binding and its relation to polyelectrolyte conformation

Binding and Clustering of Glycosaminoglycans: A Common Property of Mono- and Multivalent Cell-Penetrating Compounds

Interaction between Acridine Orange and poly(styrenesulfonic acid)

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