Interaction between Acridine Orange and poly(styrenesulfonic acid)

Abstract

The interaction between Acridine Orange and poly(styrenesulfonic acid) has been studied spectrophotometrically. The experimental results can be interpreted by an "Ising-type" model which accounts for the adsorption of dye molecules along an infinite linear chain of fixed adsorption sites. The effect of temperature and added salt on the Acridine Orange-poly(styrenesulfonic acid) has been also investigated.

Acridine orange association equilibrium in aqueous solution

“…Acridine orange (Merk für Mikroscopie) was purified as described in a previous paper (11). Spectrophotometric Measurements.…”

“…f, Spectra. Limiting extinction coefficients for the monomer species were obtained for a set of wavelengths ranging between 530 and 420 nm by extrapolation of experimental data at infinite dye dilution according to the expression E = £, + aC + bC2 + ... (at each wavelength) (11) Figure 2A is a graph of eq 11 at two characteristic wavelengths, the maximum of monomer dye (492 nm) and the isosbestic point corresponding to equilibrium 1; this point was assumed at the wavelength where a = 0 (eq 11), 471.5 nm. Some numerical extinction values are given in Table I.…”

A cation exchange based electrochemical sensor for cetyltrimethylammonium bromide detection using an acridine orange/polystyrene sulfonate system

“…The interaction of AO with anionic polyelectrolytes as models for technical applications has also received attention. [36][37][38] Herein, we report a simple and rapid approach for the electrochemical detection of CTAB based on the AO/PSS/PEI modied glassy carbon electrode (GCE); as shown in Scheme 1, PEI was rst immobilized on the electrode surface to increase the adhesion of the PSS to the substrate. Because of the electrostatic interactions between AO and PSS, AO could be stably conned on the electrode surface.…”

Elucidation of photophysical changes and orientation of acridine orange dye on the surface of borate capped gold nanoparticles using multi-spectroscopic techniques

“…Acridine orange (AO) is an important acridine dye that has potential applications in photodynamic therapy, clinical diagnosis and as a probe molecule in model biological systems to distinguish between DNA and RNA. [37][38][39][40][41][42][43] On the other hand, Au NPs coupled with organic dyes are widely used for the SERRS technique, biomolecular labeling and as immune probes. 13 In view of these considerations, AO and Au NPs have been chosen to investigate the orientation and adsorption characteristics of AO on metal nanoparticle surfaces.…”