Acridinium ester chemiluminescence: pH dependent hydrolysis of reagents and flow injection analysis of hydrogen peroxide and glutamate

Photoproduction Rates of One-Electron Reductants by Chromophoric Dissolved Organic Matter via Fluorescence Spectroscopy: Comparison with Superoxide and Hydrogen Peroxide Rates

“…Hydrogen peroxide was measured through the chemiluminescent reaction with a FeLume (Waterville Analytical Co.). For freshwater and buffered samples, the carrier was 0.1 M HCl, the buffer was 0.1 M sodium carbonate at a pH of 11.7, and the reagent acridinium ester (10-methyl-9-(p-formylphenyl) acridinium carboxylate trifluoromethanesulfonate) (AE) was prepared at 5 μM in 1 mM phosphate buffer at a pH of 3. , For higher salinity samples, the HCl carrier was increased to 0.3 M, the pH of the sodium carbonate buffer was lowered to 10.7, and the AE concentration was lowered to 1–2 μM. The FIA photomultiplier tube was set at a voltage of 950 V and an integration time of 400 ms for freshwater and buffered samples.…”

2-Methylphenyl acridine-9-carboxylate

“…This is because 9-carboxy-10-methylacridinium phenyl esters react efficiently with hydrogen peroxide in alkaline media, producing electronically excited light-emitting 10-methyl-9-acridinones (Rak et al, 1999). in immunological, medical, environmental and biochemical analyses (Kaltenbach & Arnold, 1992;Pringle, 1999;Dodeigne et al, 2000;Smith et al, 2000;Zomer & Jacquemijns, 2001;Kricka, 2003;Roda et al, 2003). This phenomenon is utilized in quantitative assays of macromolecules present in living matter, e.g.…”

Analytical Aspects of Amino, Quaternary Ammonium, Nitro, Nitroso and Related Functional Groups

“…Thus, for example, a throughput of 200 samples of glutamate per hour was achieved with LOD 0.5 mM (SNR 3) 365 . Hydrogen peroxide, generated on degradation of amino acids by L-amino acid oxidase immobilized in a reactor, can be determined by measuring the chemiluminescence in the presence of luminol (69) and hexacyanoferrate ions.…”