Acrylamide-induced adverse cerebellar changes in rats: possible oligodendrogenic effect of omega 3 and green tea

Abstract

Background: Humans are widely exposed to acrylamide (ACR) and its neurotoxicity is a significant public health issue attracting wide attention. The aim of the study was to investigate ACR-induced adverse cerebellar changes in rats and study the possible oligodendrogenic effect of omega 3 and green tea. Materials and methods: Twenty-four adult albino rats weighing 150-200 g were randomly divided into four equal groups (6 rats each): control group (Group I), the rats that received ACR 45 mg/kg/day (Group II), the rats that received ACR concomitant with omega 3 at a dosage of 200 mg/kg/day (Group III), the rats that received ACR concomitant with green tea dissolved in drinking water at a dosage of 5 g/L (Group IV). The rats were euthanized after 8 weeks of the experiment. Malondialdehyde (MDA) and glutathione (GSH) were measured in cerebellar homogenates. Sections of 5 µm thickness from specimens from the cerebellum were stained with haematoxylin and eosin, silver stain and immunohistochemical stains: platelet-derived growth factor alpha (PDGFα; for oligodendrocytes), glial fibrillary acidic protein (GFAP; for astrocytes) and BCL2 (antiapoptotic). Results: Omega 3 and green tea had improved MDA and GSH as compared to the ACR group. Histologically, the ACR group showed variable degrees of cellular degeneration. Omega 3 had induced oligodendrogenesis in Group III. The optical density of silver stain was significantly (p < 0.05) increased in Groups III and IV as compared to the ACR group. Area per cent of positive PDGFα was significantly increased in the ACR + omega 3 group as compared to the ACR group. Area per cent of positive GFAP was significantly decreased in Groups III and IV as compared to the ACR group. Area per cent of positive BCL2 was significantly increased in the omega 3-trated group as compared to the ACR group. Conclusions: Concomitant administration of omega 3 or green tea with ACR might mitigate the adverse cerebellar changes caused by ACR thanks to an oligodendrogenic effect of omega 3. (Folia Morphol 2019; 78, 3: 564-574)

Evaluation of The Ameliorative Effect of Green Tea Versus Berberine on Methyl Mercury Toxicity of Cerebellum in Adult Male Albino Rats, Histological & Immunohistochemical Study.

“…By microscopic examination , there was improvement in the histopathological findings after co-administration of GTex with MeHg. This was in accordance with Imam and Gadallah (2019) who stated that GTex MDA levels significantly dropped and GSH significantly increased after coadministration of GTex with Acrylamide-induced cerebellar toxicity. The catechins content in GTex have a significant role in scavenging free radicals.…”

Evaluation of the Postnatal Effects Induced by Diazinon on the Growth of the Mice Offspring and the Development of Their Cerebellar Cortex

“…A significant decrease in Purkinje cell number was recorded. This is consistent with Amin et al [2019] and Imam and Gadallah [2019], who explained that it might occur due to oxidative stress and free radicals formation. Meanwhile, Purkinje cells in group IIa (at birth) were displaced upward in the molecular layer and downward in the granular layer, which is described as multi-layer deposition of Purkinje cells.…”

Influence of Acrylamide Administration on the Neurochemical Characteristics of Enteric Nervous System (ENS) Neurons in the Porcine Duodenum

“…Acrylamide-induced oxidative stress may also help to mediate the activation of glial cells and the release of proinflammatory cytokines, consequently leading to neuronal damage [44]. This hypothesis is supported by numerous studies using natural and synthetic antioxidants (including vitamin C, vitamin E, green tea, and others) in the protection of neurons by acrylamide neurotoxicity [45][46][47]. Oxidative stress and a deficiency of antioxidants may also play an important role in GI tract damage caused by acrylamide.…”