Acrylic Acid Plasma Coated 3D Scaffolds for Cartilage tissue engineering applications

Abstract

The current generation of tissue engineered additive manufactured scaffolds for cartilage repair shows high potential for growing adult cartilage tissue. This study proposes two surface modification strategies based on non-thermal plasma technology for the modification of poly(ethylene oxide terephthalate/poly(butylene terephthalate) additive manufactured scaffolds to enhance their cell-material interactions. The first, plasma activation in a helium discharge, introduced non-specific polar functionalities. In the second approach, a carboxylic acid plasma polymer coating, using acrylic acid as precursor, was deposited throughout the scaffolds. Both surface modifications were characterized by significant changes in wettability, linked to the incorporation of new oxygen-containing functional groups. Their capacity for chondrogenesis was studied using ATDC5 chondroblasts as a model cell-line. The results demonstrate that the carboxylic acid-rich plasma coating had a positive effect on the generation of the glucoaminoglycans (GAG) matrix and stimulated the migration of cells throughout the scaffold. He plasma activation stimulated the formation of GAGs but did not stimulate the migration of chondroblasts throughout the scaffolds. Both plasma treatments spurred chondrogenesis by favoring GAG deposition. This leads to the overall conclusion that acrylic acid based plasma coatings exhibit potential as a surface modification technique for cartilage tissue engineering applications.

3D‐printed polymer packing structures: Uniformity of morphology and mechanical properties via microprocessing conditions

“…The low melting temperature of PLA highlights its favorable extrusion conditions, which is similar to other widely used natural materials for 3D printing processes such as poly(propylenefumarate), PCL, starch/PCL, and poly(ethylene oxide) terephthalate-co-(butylene) teraphtalate. [38,59] The printing source material should be fully melted for extrusion through the printing nozzle and the rate of cooling determines the resulting morphology. [51][52][53]60] Thus, the printing-temperature-dependent qualities of the 3Dprinted specimens were determined first as the most important process requirement, and printing speed and layer height dependencies were analyzed consecutively.…”

Plasma-digital nexus: plasma nanotechnology for the digital manufacturing age

“…Plasma-modified polymers used as a scaffold for biomedical applications have been intensively studied (Cools, et al, 2018;Joshy, et al, 2019). Indeed, surface modification using plasmas is a highly exploited and productive technique in tissue engineering and regenerative medicine.…”

Atmospheric Aerosol Assisted Pulsed Plasma Polymerization: An Environmentally Friendly Technique for Tunable Catechol-Bearing Thin Films

“…A large diversity of polymer coatings characterized by exceptional properties (e.g., antifouling Pandiyaraj et al, 2018, superhydrophobic Levasseur et al, 2012, etc.) or functionalized with various chemical groups such as epoxy (Camporeale et al, 2015), anhydride (Manakhov et al, 2012; Permyakova et al, 2018), carboxylic (Chen et al, 2018; Cools et al, 2018) or amine (Borris et al, 2007), can be produced by this process.…”