Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

Caroli, Monica De; Barozzi, Fabrizio; Renna, Luciana; Piro, Gabriella; Sansebastiano, Gian Pietro Di

doi:10.3390/membranes11040299

Cited by 7 publications

(3 citation statements)

References 55 publications

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“…The direct ER-to-vacuole route has not been well described, despite more proteins have been described to follow this route. Recently a novel ER-derived compartment -ERMEC -has been implicated in chitinase A transport to the vacuole [42]. Also, in seeds, it has been described the direct ER-transport to the PSV dependent on ERbodies.…”

Section: Discussionmentioning

confidence: 99%

PSI-mediated Vacuolar Sorting is not Disrupted in Arabidopsis <em>leb</em>-2 Mutant

Cardoso¹,

Sampaio²,

Neves³

et al. 2023

Preprint

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The endomembrane system in plant cells enables the cell to manage and coordinate a variety of membranous compartments so that they and their contents arrive at the right location. The secretory pathway takes part in this complex network and has its gateway at the Endoplasmic Reticulum. Therefore, alterations at the Endoplasmic reticulum level could condition how protein trafficking takes place or, at least, influence how such cargo leaves this organelle. With this work, we intended to assess how abnormalities at the Endoplasmic Reticulum would interfere with protein sorting and trafficking. Thus, we used a novel Arabidopsis mutant - leb-2 GFP-h -, presenting ER morphology alterations. Our results show that alterations in the leb-2 GFP-h mutant did not disrupt PSI A/B–mCherry transport to the vacuole but influences the expression of endogenous aspartic proteinases. Furthermore, the study of key-endomembrane genes expression revealed an upregulation of the SNAREs AtVAMP722 and AtVAMP723. As a whole, the leb-2 mutant seems not to interfere with vacuolar routes but may be implicated in secretion events.

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Section: Discussionmentioning

confidence: 99%

PSI-mediated Vacuolar Sorting is not Disrupted in Arabidopsis <em>leb</em>-2 Mutant

Cardoso¹,

Sampaio²,

Neves³

et al. 2023

Preprint

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show abstract

“…Plasmids used for transient transformation were prepared with a Qiagen midi-kit (Hilden, DE). The plasmid expressing GFPgl133Chi labels the unconventional traffic from the endoplasmic reticulum (ER) to the central vacuole while RFP::SYP51 efficiently labels the tonoplast and trans-Golgi networks (TGNs) . All visual analyses were conducted 18–24 h after transformation.…”

Section: Methodsmentioning

confidence: 99%

Highly Efficient Verbascoside Production from Olive (Olea europea L. var. Cellina di Nardò) In Vitro Cell Cultures

Blando,

Mita,

Di Sansebastiano

et al. 2024

J. Agric. Food Chem.

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Olive (Olea europea L.) is one of the oldest and most important fruit tree species cultivated in the Mediterranean region. Various plant tissues, drupes, and olive oil contain several phenolics (including verbascoside, although it is present in the plant at a low level) that are well-known for their highly beneficial effects on human health. An in vitro olive cell suspension culture (cultivar Cellina di Nardo, "CdN") was established, characterized for its growth and morphological features. Furthermore, a vital and relatively uniform population of protoplasts was generated from the olive suspension culture to investigate their cellular characteristics during growth. The polyphenolic extract of the in vitro "CdN" olive cells contained almost exclusively verbascoside, as revealed by the UPLC-ESI-MS analysis. The content of verbascoside reached up to 100 mg/g DW, with an average production rate of approximately 50 mg/g DW over one year of culture. This level of production has not been previously reported in a limited number of previous studies. This remarkable production of verbascoside was associated with an exceptionally high antioxidant capacity. The high level of verbascoside production and purity of the extract make this system a promising tool for secondary metabolite production.

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“…A. thaliana Nip1.1 showed to be important for membrane direct traffic from ER to tonoplast [ 13 , 25 ]. Its localization in the ER membrane suggests it is not essential for As[III] uptake.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection

Paolis

Caroli

Rojas

et al. 2022

Plants

Self Cite

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Dittrichia viscosa (L.) Greuter is gaining attention for its high genetic plasticity and ability to adapt to adverse environmental conditions, including heavy metal and metalloid pollution. Uptake and translocation of cadmium, copper, iron, nickel, lead, and zinc to the shoots have been characterized, but its performance with arsenic is less known and sometimes contradictory. Tolerance to As is not related to a reduced uptake, but the null mutation of the aquaporin Nip1.1 gene in Arabidopsis makes the plant completely resistant to the metalloid. This aquaporin, localized in the endoplasmic reticulum, is responsible for arsenite and antimony (Sb) membrane permeation, but the uptake of arsenite occurs also in the null mutant, suggesting a more sophisticated action mechanism than direct uptake. In this study, the DvNip1 gene homologue is cloned and its expression profile in roots and shoots is characterized in different arsenic stress conditions. The use of clonal lines allowed to evidence that DvNip1.1 expression level is influenced by arsenic stress. The proportion of gene expression in roots and shoots can be used to generate an index that appears to be a promising putative selection marker to predict arsenic-resistant lines of Dittrichia viscosa plants.

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Actin and Microtubules Differently Contribute to Vacuolar Targeting Specificity during the Export from the ER

Cited by 7 publications

References 55 publications

PSI-mediated Vacuolar Sorting is not Disrupted in Arabidopsis <em>leb</em>-2 Mutant

PSI-mediated Vacuolar Sorting is not Disrupted in Arabidopsis <em>leb</em>-2 Mutant

Highly Efficient Verbascoside Production from Olive (Olea europea L. var. Cellina di Nardò) In Vitro Cell Cultures

Evaluation of Dittrichia viscosa Aquaporin Nip1.1 Gene as Marker for Arsenic-Tolerant Plant Selection

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