Actin Cytoskeleton Participation in the Onset of IL-1β Induction of an Invasive Mesenchymal-like Phenotype in Epithelial MCF-7 Cells

Short Term Exposure of Beta Cells to Low Concentrations of Interleukin-1β Improves Insulin Secretion through Focal Adhesion and Actin Remodeling and Regulation of Gene Expression

“…In the basal condition (2.8 mM glucose), no effect of 0.1 ng/ml IL-1␤ was observed on FA morphology or number (Fig. 2E); nevertheless actin organization was modified with the formation of small protrusions and numerous thin filaments as previously observed in fibroblasts (33). As demonstrated in Fig.…”

“…Low Dose of IL-1␤ Affected FA Morphology and Actin Organization-Because it has been observed that IL-1␤ is involved in actin (33) and FA remodeling (34) in other cell types, we hypothesized that IL-1␤ treatment of beta cells could similarly have an effect on these key events previously implicated in insulin secretion (30,31). Confocal immunofluorescence studies of the basal membrane of glucose-stimulated rat primary beta cells revealed that exposure to 0.1 and 2 ng/ml IL-1␤ increased the percentage of actin and PXN-containing FAs with a length superior or equal to 2.5 m, with an average FA length of 2.82 Ϯ 0.16 m (0.1 ng/ml IL-1␤) and 2.83 Ϯ 0.14 m (2 ng/ml IL-1␤) compared with 1.91 Ϯ 0.08 m for the control condition (Fig.…”

“…Glucose was seen to induce phosphorylation of PTK2 and PXN, necessary for downstream activation of ERK, and induced their recruitment to newly formed protrusions at the basal membrane of beta cells. Interestingly, IL-1␤ is known to induce actin remodeling and FA maturation linked to integrin activation in fibroblasts (33,34), and FA signaling can also impact IL-1R expression, cluster these receptors at FAs, and induce ERK activation in other tissues (35). Taking all these earlier observations into consideration, we have now studied the impact of short term (2 h or less) exposure of primary beta cells to IL-1␤ with a special focus on possible beneficial effects of low concentrations of the cytokine on beta cell function and the underlying molecular mechanisms including specifically FA and actin cytoskeleton remodeling.…”

Low-Dose Paclitaxel Reduces S100A4 Nuclear Import to Inhibit Invasion and Hematogenous Metastasis of Cholangiocarcinoma

“…The small Rho GTPases are recognized as key effectors able to activate invasion and metastasis programs (20,26). In EGI-1 cells, inhibition of S100A4 nuclear import by low dose PTX significantly reduced the activation of Rho-A and Cdc-42.…”

Interleukin-1β and tumor necrosis factor-α increase stiffness and impair contractile function of articular chondrocytes

“…Indeed, both IL-1β [43][44][45] and TNF-α [46][47][48] have been shown to induce F-actin reorganization in a variety of cell types, but further changes of cell mechanical properties remain controversial. Qi et al [49] reported a decrease of tenocyte stiffness (cellular level) but an increase of tendon stiffness (tissue level) [50] in response to IL-1β; Bakker et al [30] showed that both IL-1β and TNF-α decreased the stiffness of osteocytes; and Lee et al [51] found that TNF-α increased the stiffness of all three endothelial cell lines tested.…”