Actinobacillus pleuropneumoniae Interaction With Swine Endothelial Cells

Abstract

Actinobacillus pleuropneumonia is a swine (host) specific respiratory pathogen and the etiological agent of swine pleuropneumonia which affects pigs of all ages, many being asymptomatic carriers. This pathogen has high morbidity and mortality rates which generates large economic losses for the pig industry. Actinobacillus pleuropneumoniae is a widely studied bacterium, however its pathogenesis is not yet fully understood. The prevalence of the 18 serotypes of A. pleuropneumoniae varies by geographic region, in North American area, more specifically in Mexico, serotypes 1, 3, 5b, and 7 show higher prevalence. Actinobacillus pleuropneumoniae is described as a strict extracellular pathogen with tropism for lower respiratory tract. However, this study depicts the ability of these serotypes to adhere to non-phagocytic cells, using an endothelial cell model, as well as their ability to internalize them, proposing it could be considered as an intracellular pathogen.

MS2 Virus-like Particles as a Versatile Peptide Presentation Platform: Insights into the Deterministic Abilities for Accommodating Heterologous Peptide Lengths

“…25,26 It may induce cell apoptosis during expression, thereby impacting the normal expression of the chimeric protein. Additionally, App1766, falling within the range of 200−300 aa, displayed partial solubility (Figure 2B), which might be influenced by its unique physicochemical properties as an outer membrane protein of Klebsiella pneumoniae, potentially adsorbing onto E. coli and inducing cell lysis, 27 thereby allowing partial expression in the supernatant. Moreover, despite forming inclusion bodies, the expression levels of dMS2-GST and dMS2-eGFP revealed relatively higher levels.…”

The changes of respiratory microbiome between mild and severe asthma patients

“…23 Other microbiomes were also regulated in mild and severe disease, such as Actinobacillus. Actinobacillus is a type of opportunistic pathogen resident in the human lung, and an altered proportion of them may cause the genesis of disease such as infectious pneumonia; [24][25][26] Aggregatibacter is thought to be a common pathogen and has been found to be a cause of human pneumonia. 27 Besides, Deinococcus is a type of bacteria colonized in dirt and the host gut, and limited searches found that it participated in respiratory disease.…”

Actinobacillus pleuropneumoniae, surface proteins and virulence: a review

“…On the other hand, AasP is also related to other proteins of the SPATE family of serine proteases. Plasencia-Muñoz et al ( 115 ) identified in immunoblot a band with a molecular weight of 100 kDa using a polyclonal antibody against Vat toxins, weight like the AasP protein. In a cellular interaction, the internalization of the bacteria is observed as the formation of vacuoles in endothelial cells in an in vitro cell model.…”