Actinokineospora baliensis sp. nov., Actinokineospora cibodasensis sp. nov. and Actinokineospora cianjurensis sp. nov., isolated from soil and plant litter

Abstract

Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the strains were located in the broad cluster of the genus Actinokineospora. Chemotaxonomic data [cell-wall diamino acid, meso-diaminopimelic acid; cell-wall peptidoglycan, type III (A1γ); major sugars, galactose and arabinose; major menaquinone, MK-9(H4); major fatty acid, iso-C16 : 0; major phospholipid, phosphatidylethanolamine] supported the affiliation of all six strains to the genus Actinokineospora. The results of DNA–DNA hybridization with DNA from type strains of Actinokineospora species with validly published names revealed three DNA–DNA relatedness groups. Group I (ID03-0561T) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784T, ID03-0808 and ID03-0809) formed a group with high relatedness (98–100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810T and ID03-0813) showed 58–68 % relatedness to Actinokineospora terrae NBRC 15668T and showed low relatedness (2–24 %) to the other strains studied. The description of three novel species is proposed: Actinokineospora baliensis sp. nov., for the single strain in group I (type strain ID03-0561T =BTCC B-554T =NBRC 104211T), Actinokineospora cibodasensis sp. nov., for the strains in group II (type strain ID03-0784T =BTCC B-555T =NBRC 104212T), and Actinokineospora cianjurensis sp. nov., for the strains in group III (type strain ID03-0810T =BTCC B-558T =NBRC 105526T).

Mannan endo-1,4-β-mannosidase from Kitasatospora sp. isolated in Indonesia and its potential for production of mannooligosaccharides from mannan polymers

“…Molecular identification of the ID04-0555 strain was conducted based on the 16S rRNA gene as established by Lisdiyanti et al (2010). The 16S rRNA gene was amplified via polymerase chain reaction (PCR) technique using a pair of 9F and 1510R primers (Additional file 1: Table S1) (Burggraf et al 1992).…”

“…Fortunately, Indonesia is also rich in terrestrial microbes, particularly in actinomycetes. Several scientists have reported that actinomycetes isolated from Indonesian soils and leaf-litters represent new species and demonstrate genus diversity (for example, Otoguro et al 2009, 2011; Lisdiyanti et al 2010; Yamamura et al 2010). Extensive exploration of industrially useful enzymes has been conducted from these actinomycetes, such as inulin fructotransferase (Pudjiraharti et al 2011, 2014).…”

Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated Bacterium Actinokineospora sp. Strain EG49

“…The genus Actinokineospora belongs to the family Actinosynnemataceae under the phylum Actinobacteria (2). Members of Actinokineospora are widespread in terrestrial environments, including soil and plants (3, 4), and were recently isolated from marine sponges (1). So far only two draft genomes of the genus Actinokineospora are available (BioProject identification [ID] 188797 and 174972 [http://www.ncbi.nlm.nih.gov/]).…”

Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil

“…Currently, this genus, which belongs to the family of Pseudonocardiaceae, contains thirteen species with validly published names: Actinokineospora riparia (Hasegawa 1988), Actinokineospora inagensis, Actinokineospora globicatena, Actinokineospora terrae, Actinokineospora diospyrosa (Tamura et al 1995), Actinokineospora auranticolor, Actinokineospora enzanensis (Otoguro et al 2001), Actinokineospora fastidiosa (Labeda et al 2010), Actinokineospora baliensis, Actinokineospora cibodasensis, Actinokineospora cianjurensis (Lisdiyanti et al 2010), Actinokineospora soli (Tang et al 2012) and Actinokineospora bangkokensis (Intra et al 2013).…”