Actinomarinicola tropica gen. nov. sp. nov., a new marine actinobacterium of the family Iamiaceae, isolated from South China Sea sediment environments

International Journal of Systematic and Evolutionary Microbiology

et al. 2022

An actinobacterium, designated strain EGI L10124T, was isolated from saline lake sediment collected in Xinjiang province, PR China. The taxonomic position of the isolate was determined based on polyphasic taxonomic and phylogenomic analyses. Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that strain EGI L10124T formed a distinct clade with Rhabdothermincola sediminis SYSU G02662T, with a shared sequence identity of 95.2 %. The novel isolate could be distinguished from species in the genus Rhabdothermincola by its distinct phenotypic, physiological and genotypic characteristics. The cells of strain EGI L10124T were aerobic, Gram-stain-positive and short rod-shaped. Optimal growth conditions of strain EGI L10124T on marine agar 2216 were registered at pH 8.0 at 37 °C. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The major respiratory quinone was MK-9 (H8), while the major fatty acids were iso-C16 : 0, C17 : 0 and C16 : 0. The polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. Based on the genome sequence of strain EGI L10124T, it appears that the G+C content of the novel isolate was 71.8 mol%. According to our data, strain EGI L10124T represents a new species of the genus Rhabdothermincola , for which the name Rhabdothermincola salaria sp. nov. is proposed. The type strain of the proposed novel isolate is EGI L10124T (=CGMCC 1.19113T=KCTC 49679T).

Section: Full-textmentioning

confidence: 99%

mentioning

confidence: 99%

Rhabdothermincola salaria sp. nov., a novel actinobacterium isolated from a saline lake sediment

Gao

Fang

International Journal of Systematic and Evolutionary Microbiology

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

“…The effect of pH on growth was investigated in MB from pH 3.0 to 10.0 (pH 3.0, 4.0, 5.0, 5.5, 6.0, 7.0, 8.0, 9.0, 10.0) using the buffer system described by He et al . [7]. OD 600 values of the culture were measured after 0, 4, 8, 16, 24, 36, 48, 72 and 96 h incubation at 28 °C.…”

Section: Full-textmentioning

confidence: 99%

“…The effects of different salt concentrations on growth were detected by using different concentrations of NaCl, MgCl•6H 2 O or sea salt (Sarrebourg) from 0 to 12.0 % (0, 0.5, 1.0, 2.0, 3.0, 3.5, 4.0, 5.0, 10.0, 12.0 %, w/v). The effect of pH on growth was investigated in MB from pH 3.0 to 10.0 (pH 3.0, 4.0, 5.0, 5.5, 6.0, 7.0, 8.0, 9.0, 10.0) using the buffer system described by He et al [7]. OD 600 values of the culture were measured after 0, 4, 8, 16, 24, 36, 48, 72 and 96 h incubation at 28 °C.…”

mentioning

confidence: 99%

Acidiluteibacter ferrifornacis gen. nov., sp. nov., a new member of the Flavobacteriales from Tielu Harbour, Hainan, PR China

Gui

Huang

et al. 2020

A novel bacterial strain of the family ‘Vicingaceae’ was isolated from mangrove of Tielu Harbour, Hainan, PR China. Strain S-15T was a Gram-stain-negative, short-rod-shaped, yellow-pigmented that could grow at 10–42 °C (optimum, 26–35 °C), at pH 5.0–9.0 (optimum, pH 5.5) and in 0.5–10.0 % w/v sea salt (optimum, 3.5–4.0 %). Cells of strain S-15T were 0.9–1.4 µm long, 0.8–0.9 µm wide, catalase-positive and oxidase-positive. Colonies on modified marine agar 2216 were 0.5–2.0 mm in diameter after incubation for 72 h at 28 °C. Analysis of 16S rRNA gene sequences revealed that strain S-15T was most closely related to Vicingus serpentipes ANORD5T (89.8 %). The major respiratory quinone of strain S-15T was menaquinone MK-7, and the dominant fatty acids were C15:0 iso, C15:1 iso G and C17:0 iso 3-OH. The major polar lipids were two unidentified aminolipids, phosphatidylethanolamine and six unidentified lipids. Analyses showed that the genome size was 3.52 Mb and the DNA G+C content was 35.6 mol%, which were higher than V. serpentipes ANORD5T with 2.92 Mb genome size and 31.0 mol% G+C content, respectively. Based on morphological, physiological and phylogenetic data, strain S-15T is considered a type strain of a new species and a new genus of the family ‘Vicingaceae’ for which the name Acidiluteibacter ferrifornacis gen. nov., sp. nov. is proposed. The type strain of Acidiluteibacter ferrifornacis is S-15T (=MCCC 1K03817T=JCM 33804T).

“…[1], and the type strain Iamia majanohamensis F12 T was isolated from a sea cucumber in Japan. At the time of writing, three genera were included in the family Iamiaceae , Iamia [1], Aquihabitans [2] and Actinomarinicola [3], and they were isolated from sea cucumber, reservoir water and oceanic sediment, respectively. Members of the family Iamiaceae share the same short rod-shape and MK-9 (H 6 ) and MK-9 (H 8 ) as their major respiratory quinones.…”

Section: Full-textmentioning

confidence: 99%

Rhabdothermincola sediminis gen. nov., sp. nov., a new actinobacterium isolated from hot spring sediment, and emended description of the family Iamiaceae

Jiao

International Journal of Systematic and Evolutionary Microbiology

et al. 2019

One thermophilic bacterium, designated strain SYSU G02662T, was isolated from hot spring sediment sampled in Tibet, PR China. Polyphasic taxonomic analyses and whole-genome sequencing were used to determine the taxonomy position of the strain. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SYSU G02662T showed the highest sequence similarity to Actinomarinicola tropica SCSIO 58843T (95.1 %). The strain could be differentiated from other species of the family Iamiaceae by its distinct phenotypic and genotypic characteristics. Cells of strain SYSU G02662T were aerobic, Gram-staining-positive and short rodshaped. Growth occurred optimally at 45 °C and pH 7.0. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The respiratory quinone was MK-9 (H8), while the major fatty acids (>10 %) were C16 : 0, C17 : 0, C18 : 0 and iso-C16 : 0. The detected polar lipids included diphosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. The G+C content of the genomic DNA was 70.5 % based on the draft genomic sequence. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU G02662T represents a novel species of a novel genus in the family Iamiaceae , for which the name Rhabdothermincola sediminis gen. nov., sp. nov. is proposed. The type strain of the proposed novel species is SYSU G02662T (=CGMCC 4.7688T=KCTC 49500T).