Actinomycete complexes in the rhizosphere of winter rye on soddy podzolic soil

Широких, И. Г.; Merzaeva, O. V.

doi:10.1007/s11021-005-0056-1

Cited by 4 publications

(3 citation statements)

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“…Actinobacteria are widely distributed in terrestrial and marine habitats (Hayakawa et al 2000;Terkina et al 2002;Shirokikh and Merzaeva 2005;Ward and Bora 2006), and are important contributors of bioactive compounds including antibiotics, antitumor agents, enzymes and enzyme inhibitors (Lazzarini et al 2000). In fact, over two-thirds of natural product antibiotics are produced by actinomycetes (Strohl 2003).…”

Section: Introductionmentioning

confidence: 99%

Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

Sun

Dai

Jiang

et al. 2010

Antonie van Leeuwenhoek

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In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization, 16S rDNA library construction, 16S rDNA-restriction fragment length polymorphism (rDNA-RFLP) and phylogenetic analysis. A total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phylogenetic analysis on the basis of their 16S rRNA gene sequences. Results showed that the 24 isolates were assigned to six genera including Salinispora, Gordonia, Mycobacterium, Nocardia, Rhodococcus and Streptomyces. This is the first report that Salinispora is present in a marine sponge from the South China Sea. Subsequently, 26 rDNA clones were selected from 191 clones in an Actinobacteria-specific 16S rDNA library of the H. perleve sample, using the RFLP technique for sequencing and phylogenetic analysis. In total, 26 phylotypes were clustered in eight known genera of Actinobacteria including Mycobacterium, Amycolatopsis, Arthrobacter, Brevibacterium, Microlunatus, Nocardioides, Pseudonocardia and Streptomyces. This study contributes to our understanding of actinobacterial diversity in the marine sponge H. perleve from the South China Sea.

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Section: Introductionmentioning

confidence: 99%

Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

Sun

Dai

Jiang

et al. 2010

Antonie van Leeuwenhoek

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“…I.G. Shirokikh et al [19] found in the rye rhizosphere a significant number of actinomycetes; their species composition and number changed during the ontogeny of plants. The dominant position was occupied by streptomycetes.…”

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Phylogenetic Structure of Community of Procariots of Soddy-Podzolic Soil Under the Cover of Winter Rye Is Not Influenced by Agrotechnics

Филиппова¹,

Kruglov²,

Andronov³

2018

S-h. biol.

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Soil microbial communities are complex multicomponent systems that form under the influence of a wide range of factors, among them-soil type, plant species, climate, agricultural technology-in general, determining the physical and chemical characteristics of the environment. The plant, according to many researchers, is the main factor determining the structure of the soil microbial community, due to the extensive number of compounds released into the soil. There is still a discussion about the specific nature of the action of various plants on soil microbiome, which is very important both for understanding the mechanism of interaction of microorganisms and plants, and for building optimal crop rotations, as well as organizing measures to protect agricultural crops from phytopathogenic microorganisms and pests. Winter rye is one of the few crops that can grow continuously for decades. It has a powerful root system, comparable to the biomass of the above-ground part of plants. The root excretions of winter rye reach 21 % of the synthesized plant mass. This paper presents the results of research aimed at studying the phylotypical structure and diversity of prokaryotic microorganisms in rye crops grown in permanent culture and six-field crop rotation for almost 100 years, in the long-term multifactorial field experiment of the Moscow Timiryazev Agricultural Academy. The aim of our work was to study the influence of various agricultural technicians such as crop rotation and liming, under the conditions of a long field experiment on the phylogenetic structure of prokaryotic microorganisms in rye crops. The results of the high-throughput DNA sequencing of the soil microbiome and the subsequent analysis of the phylogenetic structure and diversity of the prokaryotic microorganisms of the sod-podzolic soil under the conditions of perennial rye culture showed that the plant is one of the key factors in the formation of the prokaryotic community. Regardless of the agrotechnical methods under the cover of winter rye, the same core structure of prokaryotes, including a small number of types of proteobacteria and actinobacteria, develops in the earing phase. The dominant position among them is occupied by the bacteria of the Rhizobiaceae family, which in this case is to some extent related to the history of the experimental field. Apparently, the bacteria of this family and, above all, the nodule bacteria, find favorable conditions for their development in the rye rhizosphere. It is possible that a kind of associative symbiosis is formed between them, which was observed by some authors with other cereal crops. In this connection, studies of the viability of Rhizobiaceae in winter rye crops, and their evolution to associative endosymbiotic relationships with rye in the course of a long coexistence are of undoubted interest. The effect of liming on the genetic structure of the prokaryote community of acidic soils may be different. At the same time, apparently, the specific type of plants, as well as the history of the field (crop...

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“…).O filo Actinobacteria é o maior clado filogenético de bactéria. São microorganismos Gram-positivos com alto conteúdo G+C na constituição genômica(SUN et al, 2010)e são largamente distribuídos em habitats terrestres e marinhos apresentando importantes fontes de compostos bioativos que incluem antibióticos, agentes antitumorais, enzimas e inibidores enzimáticos(HAYAKAWA et al, 2000;TERKINA et al, 2002;SHIROKIKH;MERZAEVA, 2005;WARD, BORA 2006).…”

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Bioprospecção de actinobactérias associadas à esponja marinha Aplysina fulva: isolamento, caracterização e produção de compostos bioativos

Silva¹

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Bioprospecting of actinobacteria associated with marine sponge Aplysina fulva: isolation, characterization and production of bioactive compoundsActinobacteria are producers of important pharmacological compounds. About 70% of natural antibiotics are derived from these microorganisms. However, the use of natural compounds are still limited in the agricultural industry, even considering that synthetic pesticides are less effective against pathogens and weed plants. This study describes the diversity of actinobacteria associated with the marine sponge Aplysina fulva and their potential as producers of bioactive compounds with fungicidal and herbicidal properties. In this study, a total of 21 actinomycetes were isolated with the use of selective media. Phylogenetic analyzes based on partial sequencing of the gene encoding for 16S rRNA showed that these microorganisms belong to eight Actinobacteria genera, including Kocuria, Citricoccus, Terrabacter, Gordonia, Agrococcus, Tsukamurella, Brevibacterium and Streptomyces. The extracts of all isolates were tested for the production of secondary metabolites with fungicidal properties against the following phytopathogenic fungi: of Pythium aphanidermatum, Phytophthora capsici and Magnaporthe grisea. The crude extract of 43% of the isolates showed fungicidal activity for at least one of the pathogens. The chemical profiles of the actinobacteria extracts with positive bioactivity were similar even among different genus. The metabolites of Streptomyces ASPSP 103 were more efficient because of the strong inhibition against all tested pathogens. So, the isolate ASPSP 103 was selected and tested for herbicide activity through screening for algaecide activity towards microalgae Selenastrum capricornutum. We believe that actinobacteria associated with marine sponges play a role in chemical defense against algae that can obstruct the pores, choking the animal. These algaecides compounds possibly have herbicide action. Activity of the Streptomyces ASPSP 103 crude extract against S. capricornutum was observed. In addition, it was observed a weak pre-emergence herbicide activity on Lactuca sativa (dicot) and a strong inhibition in Agrostis stolonifera (monocot). The purification of the crude extract to isolate the bioactive compound was guided by bioassay against Pythium aphanidermatum, a fast growing oomycete and sensitive to metabolites from ASPSP 103 previously tested. The butenolide compound was identified with pre-emergence herbicidal activity against Agrostis stolonifera (IC 50 33.43 µg/mL). This is the first report of butenolide activity with herbicide activity. Taxonomy studies showed that the phylogenetic, morphological and chemical characteristics of the isolated ASPSP 103 are consistent with the Streptomyces genus. Then, considering some differences in taxonomic parameters, ASPSP 103 T was proposed as line type for a new species of Streptomyces, for which the name Streptomyces atlanticus sp. nov. was suggested. These results emphasize the potential of marine Streptomyces to p...

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Actinomycete complexes in the rhizosphere of winter rye on soddy podzolic soil

Cited by 4 publications

References 2 publications

Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

Culture-dependent and culture-independent diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve from the South China Sea

Phylogenetic Structure of Community of Procariots of Soddy-Podzolic Soil Under the Cover of Winter Rye Is Not Influenced by Agrotechnics

Bioprospecção de actinobactérias associadas à esponja marinha Aplysina fulva: isolamento, caracterização e produção de compostos bioativos

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