Actinomycetes: an unexplored microorganisms for plant growth promotion and biocontrol in vegetable crops

Chaurasia, Aalok Ranjan; Meena, B. L.; Tripathi, Ajay; Pandey, Koshlendra Kumar; B, A; Singh, Bijendra

doi:10.1007/s11274-018-2517-5

Cited by 67 publications

(40 citation statements)

References 74 publications

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“…Therefore, PGPR can play an essential role in the sustainable agriculture industry. Specifically, actinobacteria can be more suitable for dry, arid soils and extreme hot environments (Chaurasia et al, 2018) such as those in the UAE (Saeed et al, 2017;Kamil et al, 2018). A recent study has compared growth promotion of S. bigelovii between rhizospherecompetent versus rhizosphere-non-competent actinobacterial strains after irrigation with seawater (El-Tarabily et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation

et al. 2020

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Salicornia bigelovii is a promising halophytic cash crop that grows in seawater of the intertidal zone of the west-north coast of the UAE. This study assess plant growth promoting (PGP) capabilities of halotolerant actinobacteria isolated from rhizosphere of S. bigelovii to be used as biological inoculants on seawater-irrigated S. bigelovii plants. Under laboratory conditions, a total of 39 actinobacterial strains were isolated, of which 22 were tolerant to high salinity (up to 8% w/v NaCl). These strains were further screened for their abilities to colonize S. bigelovii roots in vitro; the most promising ones that produced indole-3-acetic acid, polyamines (PA) or 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD) were selected for rhizosphere-competency under naturally competitive environment. Three outstanding rhizosphere-competent isolates, Streptomyces chartreusis (Sc), S. tritolerans (St), and S. rochei (Sr) producing auxins, PA and ACCD, respectively, were investigated individually and as consortium (Sc/St/Sr) to determine their effects on the performance of S. bigelovii in the greenhouse. Individual applications of strains on seawater-irrigated plants significantly enhanced shoot and root dry biomass by 32.3-56.5% and 42.3-71.9%, respectively, in comparison to noninoculated plants (control). In addition, plants individually treated with Sc, St and Sr resulted in 46.1, 60.0, and 69.1% increase in seed yield, respectively, when compared to control plants. Thus, the synergetic combination of strains had greater effects on S. bigelovii biomass (62.2 and 77.9% increase in shoot and root dry biomass, respectively) and seed yield (79.7% increase), compared to the control treatment. Our results also showed significant (P < 0.05) increases in the levels of photosynthetic pigments, endogenous auxins and PA, but a reduction in the levels of ACC in tissues of plants inoculated with Sc/St/Sr. We conclude that the consortium of isolates was the most effective treatment on S. bigelovii growth; thus confirmed by principal component and correlation analyses. To this best of our knowledge, this is the first report about

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Section: Discussionmentioning

confidence: 99%

Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation

et al. 2020

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“…Increasing the presence of antibiotic-producing bacteria such as Streptomyces species in agricultural soils has been shown to suppress fungal pathogens in numerous recent studies, (e.g. Cha et al 2016, Inderbitzin et al 2018, Ren et al 2018, Zhou et al 2018) and some Streptomyces endophyte species are marketed as fungal growth suppressors (Chaurasia et al 2018, Rey and Dumas 2017, Viaene et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Streptomycesendophytes promote the growth ofArabidopsis thaliana

Newitt

Rassbach

et al. 2019

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19 20 21 22 23 101 10% (v/v) glycerol and serial dilutions were spread onto either soya flour mannitol (SFM) agar, 102 starch casein agar, or minimal medium agar containing sodium citrate (Lebeis et al 2015).103Plates were incubated at 30C for up to 14 days. Colonies resembling streptomycetes were re-104 streaked onto SFM agar and identified by 16S rRNA gene PCR amplification and sequencing 105 with universal primers PRK341F and MPRK806R (see Table S1 for primers, plasmids and 106 strains used in this work). Streptomyces strains were maintained on SFM agar (N1, N2, M2, 107 M3 and S. coelicolor M145), Maltose/Yeast extract/Malt extract (MYM) agar with trace 108 5 elements (L2) or ISP2 agar (S. lydicus strains); media recipes are detailed in Table S2. Spore 109 stocks were made as described previously (Kieser et al 2000). 110 111 Genome sequencing and analysis. High quality genome sequences were obtained for newly 112 isolated strains N1, N2, M2, M3, and L2, as well as three known strains of Streptomyces 113 lydicus; one isolated from the commercial plant growth-promoting product Actinovate and two 114 additional S. lydicus strains (ATCC25470 and ATCC31975) obtained from the American Type 115 Culture Collection. Strains were sequenced using PacBio RSII sequencing technology at the 116 131 isolated from a patient at the Norfolk and Norwich University Hospital UK (Qin et al 2017), 132Escherichia coli and Pseudomonas syringae DC3000 were grown overnight in 10 ml Lysogeny 133 Broth (LB) (1% tryptone, 0.5% NaCl, supplemented with 0.1% glucose for P. syringae). These 134 were sub-cultured 1 in 20 (v/v) for a further 4 hours at 30°C for P. syringae and 37°C for E. 135 coli. These cultures were then used to inoculate 100 ml of molten LB (0.5% agar, plus 0.1% 136 glucose for P. syringae), of which 3 ml was used to overlay agar plates containing Streptomyces 137 colonies. Plates were incubated for 48 hours at 30C, and bioactivity was indicated by a clear 138 halo around the Streptomyces colony. For bioassays using the fungal strains Lomentospora 139 prolificans or Gaeumannomyces graminis, Streptomyces species were grown for 7 days, and 140 then a plug of the fungus (grown on potato glucose agar, Sigma Aldrich, for 14 days) was 141

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“…Notably, Actinomycetes can utilize nitrogenase that catalyzes conversion of nitrogen to nitrate, which is easily absorbed by the plant roots. In addition, Actinomycetes play an active role in carbon cycling by decomposing organic matter, promotes the formation of soil aggregates and improves soil structure, all of which are conducive to plant growth and development (Chaurasia et al 2018). The relative abundance of Proteobacteria and Chloro exi in the soil of Aohan soil were higher than that of Kangsai and Victoria.…”

Section: Discussionmentioning

confidence: 99%

Comparison of rhizosphere soil microbial diversity of different introduced alfalfa varieties

Ma¹,

Qi²,

Yu³

et al. 2020

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Alfalfa (Medicago sativa L.) is an important forage legume in farming and animal husbandry systems. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and alfalfa growth characteristics and soil physical and chemical properties induced by different cultivars alfalfa (Victoria, Kangsai, Aohan) in the grey desert soil. The results showed that the diversity of bacterial and fungal in Victoria was higher, and the bacterial diversity was significantly lower for alfalfa with Aohan than for the others, and the fungal diversity was lower for alfalfa with Kangsai than for the others. Heatmap showed that total nitrogen, fresh weight, pH and organic have significantly affect fungal community structure, whereas pH and organic carbon also significant effects on bacterial community structure. LefSe analysis showed that the growth adaptability of introduced alfalfa is mainly related to fungal and bacterial species, and the beneficial microorganisms with significant differences and relative high abundance are significantly enriched in Victoria. Pathogens with high relative abundance are mainly concentrated in Aohan alfalfa soil. Based on our findings, Victoria is the high-yield alfalfa suitable for planting in gray desert soil, while planting Kangsai and Aohan alfalfa needs probiotic for adjuvant.

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Actinomycetes: an unexplored microorganisms for plant growth promotion and biocontrol in vegetable crops

Cited by 67 publications

References 74 publications

Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation

Halotolerant Marine Rhizosphere-Competent Actinobacteria Promote Salicornia bigelovii Growth and Seed Production Using Seawater Irrigation

Streptomycesendophytes promote the growth ofArabidopsis thaliana

Comparison of rhizosphere soil microbial diversity of different introduced alfalfa varieties

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