Action of 5-Bromodeoxyuridine on the Induction of Haemoglobin Synthesis in Mouse Leukaemia Cells resistant to 5-BUdR

Ostertag, Wolfram; Crozier, T. A.; Kluge, N.; Melderis, H.; Dube, Shyam K.

doi:10.1038/newbio243203a0

Cited by 54 publications

(24 citation statements)

References 13 publications

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“…Similar results have been reported recently by other workers (6,4). Estimation of globin mRNA levels by in situ hybridization shows that this reduction in hemoglobin formation is due to greatly reduced levels of globin mRNA in cells treated with DMSO and BUdR as compared with DMSO alone (Table IV).…”

Section: E F F E C T O F B R O M O D E O X Y U R I D I N Esupporting

confidence: 80%

IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATION

Conkie

Affara

Harrison

et al. 1974

The Journal of Cell Biology

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In the accompanying paper (1) in situ hybridization of globin DNA copy (cDNA) to fetal liver was used to elucidate the process of globin messenger RNA (mRNA) formation in erythroid precursor cells. The essential conclusions were also confirmed by conventional hybridization studies. In the present paper, the same dual approach is applied to a different experimental system in which hemoglobin synthesis in Friend virus-transformed mouse cells is induced by treatment with dimethylsulfoxide (DMSO) (2). The results obtained by in situ and conventional hybridization agree in showing that there is a minimum period of treatment with DMSO required in order that globin mRNA and hemoglobin may accumulate. M A T E R I A L S AND METHODS Cell CultureFriend cells (clone 707) and lymphoma cells (L 5178Y) were grown in Ham's FI2 medium (Flow Laboratories, Irvine, Scotland), supplemented with minimal essential medium amino acids, penicillin (60 U/ml), and 10-15% horse serum and buffered with HEPES or bicarbonate. LS cells were grown in Eagle's minimal essential medium plus 5% calf serum.All other methods are fully described in the accompanying paper (1). RESULTS

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Section: E F F E C T O F B R O M O D E O X Y U R I D I N Esupporting

confidence: 80%

IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATION

Conkie

Affara

Harrison

et al. 1974

The Journal of Cell Biology

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“…It has previously been demonstrated that BUdR interferes with the DMSOinduced differentiation of Friend leukaemia cells (Ostertag et al, 1973; and that this inhibition results from interference with the accumulation of globin mRNA (Conkie et al, 1974;Preisler et al, 1973). These observations, taken together with those reported here, strongly suggest that these leukaemic cells continue to synthesize and accumulate globin mRNA after removal of DMSO from the culture medium and that iinterference with this process inhibits differentiation during secondary culture in DMSO-free medium.…”

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confidence: 77%

BUDR inhibition of post-DMSO-induced erythroleukaemia cell differentiation in vitro

Preisler¹

1976

Br J Cancer

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“…Because similar "pre-f" peaks have been observed by others (2,38) and were thought to be related to hemoglobin (2), rabbit serum against DBA/2 mouse globin was used to immunoprecipitate pooled fractions eluted in "pre-3" regions from the Me2SO-and Me2SO+IF cell lysates. Table 2 shows that 63% of the proteins eluted in the "pre-f" region of profile B (Me2SO-globin) were precipitated by globin antiserum as compared to 78.7% of globin profile C (Me2SO+IF).…”

Section: Resultsmentioning

confidence: 99%

Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.

Rossi¹,

Dolei²,

Cioé³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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The addition of appropriate doses of interferon (IF) to cultures of Friend erythroleukemic cells inhibits dimethyl sulfoxide (Me2SO)-stimulated erythroid differentiation. In this study, the synthesis of heme, hemoglobin, and globin mRNA in Me2SO-stimulated cultures, with or without IF added, was compared. Although the hemoglobin content in Me2SO+IF-treated cultures was reduced 6- to 9-fold compared to that of cultures treated with Me2SO alone, there was less than a 2-fold decrease in the amount of heme accumulated. Globin mRNA, although unchanged in size or base sequence, was reduced in content in the Me2SO+IF cultures. The level of reduction of globin mRNA was insufficient to account for the lack of globin synthesis. Thus, it appears that IF may operate on two levels--one involving the transcription of globin mRNA and the other involving its translation.

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Action of 5-Bromodeoxyuridine on the Induction of Haemoglobin Synthesis in Mouse Leukaemia Cells resistant to 5-BUdR

Cited by 54 publications

References 13 publications

IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATION

IN SITU LOCALIZATION OF GLOBIN MESSENGER RNA FORMATION

BUDR inhibition of post-DMSO-induced erythroleukaemia cell differentiation in vitro

Inhibition of transcription and translation of globin messenger RNA in dimethyl sulfoxide-stimulated Friend erythroleukemic cells treated with interferon.

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