1996
DOI: 10.1111/j.1476-5381.1996.tb15754.x
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Action of 5‐hydroxytryptamine in facilitating N‐methyl‐D‐aspartate depolarization of cortical neurones mimicked by calcimycin, cyclopiazonic acid and thapsigargin

Abstract: 1 The ability of calcimycin, cyclopiazonic acid and thapsigargin to facilitate the N-methyl-D-aspartate (NMDA)-mediated depolarization of cortical projection neurones was investigated by use of grease-gap recording and the results compared with the facilitation that results from activation of 5-hydroxytryptamine2A receptors.2 Calcimycin (0.25 to 3 gM), cyclopiazonic acid (5 to 30 gM), and thapsigargin (10 to 300 nM) reversibly facilitated the NMDA (50 gM)-induced depolarization in the presence of tetrodotoxin.… Show more

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Cited by 8 publications
(7 citation statements)
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References 63 publications
(123 reference statements)
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“…Other than the use of cortical rather than hippocampal slices, a methodological difference that might account for the apparent disparity between the present observations and previous data on the histamine‐induced facilitation was our use of ACSF containing 1.3 m m Mg 2+ rather than nominally Mg 2+ ‐free medium. Hypomagnesia eliminates the facilitation induced by 5‐HT and phenylephrine (Rahman & Neuman, 1996b). Therefore, the effects of histamine were re‐examined in nominally Mg 2+ ‐free ACSF.…”
Section: Resultsmentioning
confidence: 99%
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“…Other than the use of cortical rather than hippocampal slices, a methodological difference that might account for the apparent disparity between the present observations and previous data on the histamine‐induced facilitation was our use of ACSF containing 1.3 m m Mg 2+ rather than nominally Mg 2+ ‐free medium. Hypomagnesia eliminates the facilitation induced by 5‐HT and phenylephrine (Rahman & Neuman, 1996b). Therefore, the effects of histamine were re‐examined in nominally Mg 2+ ‐free ACSF.…”
Section: Resultsmentioning
confidence: 99%
“…Therefore, the effects of histamine were re‐examined in nominally Mg 2+ ‐free ACSF. To compensate for the larger NMDA‐induced depolarization expected in Mg 2+ ‐free medium (Rahman & Neuman, 1996b), the concentration of NMDA was reduced to 25 μ m . Following at least a 2 h wash in Mg 2+ ‐free ACSF, histamine (10 μ m ) induced a facilitation of the NMDA depolarization (Figure 4).…”
Section: Resultsmentioning
confidence: 99%
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“…One might posit that the ineffectiveness of trans ‐ACPD in Mg 2+ ‐free Ringer's solution reflects the G‐protein‐coupled receptor's need for cytosolic Mg 2+ ions in order to function effectively ( El‐Beheiry & Puil, 1990 ; Rahman & Neuman, 1996b ). But, in the present experiments the NMDA channel blockers memantine and MK‐801 were able to substitute in large measure for Mg 2+ ions.…”
Section: Discussionmentioning
confidence: 99%