Action of adrenocorticotrophin on a preparation of isolated cells from rat adrenals

Kinetics and dose—response characteristics of adenosine 3′,5′‐monophosphate production by isolated rat adrenal cells stimulated with adrenocorticotrophic hormone

“…We found that cyclic AMP levels, measured by a highly sensitive saturation analysis assay, rose before any detectable increase in steroidogenesis. The sensitivity and magnitude of the steroidogenesis response to ACTH by this system has previously been reported [6]. We now present the dose-response characteristics between ACTH and adrenal cyclic AMP production.…”

“…Isolated adrenal cells were prepared by collagenase digestion of decapsulated rat adrenal glands [6]. Cell suspensions were incubated in 1 ml samples which contained cells isolated from the equivalent of one adrenal.…”

The mode of adrenocorticotrophin action in stimulating steroidogenesis: the obligatory role of adenosine 3′:5′-cyclic monophosphate and the involvement of rapidly-turning-over protein

“…Two ofthese aspects, namely the role of cyclic AMP and the involvement of rapidlyturning-over protein, are the subject of this report. The cyclic AMP content of collagenase-disaggregated rat adrenal cells (Richardson & Schulster, 1970) incubated with ACTH was assayed after Dowex chromatography (Montague & Cook, 1971) by a modification of the saturation-analysis procedure of Brown et al (1971). A sigmoid log(dosage)-response curve was obtained (EDso 5 x 10-3 i.u.…”