Action of Fusicoccin in vivo: Physiological and Biochemical Consequences

Marrè, E.; Marré, Maria Teresa; Romani, Giulia

doi:10.1007/978-3-642-73178-5_12

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…It has been suggested that the role of the FCBP, in vivo, is not to bind FC but some other essential function (6,10,19). It was a surprise, then, to find that FCBP activity was nearly absent from cells in the hook region.…”

Section: Discussionmentioning

confidence: 99%

“…The first question addressed here is whether the peak in elongation in mung bean hypocotyls is paralleled by a peak of PM ATPase activity. Because hypocotyl development progresses from apex to base, with the youngest, meristematic cells contained in the hook, the growth rate and PM ATPase activity were compared in successive sections along the hypocotyl, starting with the hook region.Proton excretion via the PM ATPase is greatly enhanced in most higher plant cells by the fungal toxin FC (19,24 does not bind to the catalytic peptide of the ATPase, but to a separate FCBP (1,5,6,20). It has been suggested that the FCBP is a constitutive regulatory subunit of the ATPase (5, 10); if so, one would expect a constant ratio between ATPase and FCBP activities.…”

mentioning

confidence: 99%

“…Proton excretion via the PM ATPase is greatly enhanced in most higher plant cells by the fungal toxin FC (19,24 does not bind to the catalytic peptide of the ATPase, but to a separate FCBP (1,5,6,20). It has been suggested that the FCBP is a constitutive regulatory subunit of the ATPase (5, 10); if so, one would expect a constant ratio between ATPase and FCBP activities.…”

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confidence: 99%

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Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls.

Basel

Cleland²

1992

Plant Physiol.

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A comparison has been made of the developmental gradients along a mung bean (Vigna radiata L.) hypocotyl of the growth rate, plasma membrane ATPase, and fusicoccin-binding protein (FCBP) activity to determine whether they are interrelated. The hook and four sequential 7.5 millimeter segments of the hypocotyl below the hook were cut. A plasma membrane-enriched fraction was isolated from each section by aqueous two-phase partitioning and assayed for vanadate-sensitive ATPase and FCBP activity. Each gradient had a distinctive and different pattem. Endogenous growth rate was maximal in the second section and much lower in the others. Vanadate-sensitive ATPase activity was maximal in the third section, but remained high in the older sections. Amounts of ATPase protein, shown by specific antibody binding, did not correlate with the amount of vanadate-sensitive ATPase activity in the three youngest sections. FCBP activity was almost absent in the first section, then increased to a maximum in the oldest sections. These data show that the growth rate is not determined by the ATPase activity, and that there are no fixed ratios between the ATPase and FCBP.As cells develop, they undergo changes in physiological potential and in their spectrum of proteins. The challenge is to relate changes in proteins to changes in physiological potential. Cells in a hypocotyl go through a period of elongation prior to maturation (7). This elongation is due, at least in part, to auxin-induced wall acidification, mediated by the PM2 ATPase (1 1). The first question addressed here is whether the peak in elongation in mung bean hypocotyls is paralleled by a peak of PM ATPase activity. Because hypocotyl development progresses from apex to base, with the youngest, meristematic cells contained in the hook, the growth rate and PM ATPase activity were compared in successive sections along the hypocotyl, starting with the hook region.Proton excretion via the PM ATPase is greatly enhanced in most higher plant cells by the fungal toxin FC (19,24 does not bind to the catalytic peptide of the ATPase, but to a separate FCBP (1,5,6,20). It has been suggested that the FCBP is a constitutive regulatory subunit of the ATPase (5, 10); if so, one would expect a constant ratio between ATPase and FCBP activities. The second question addressed here is the quantitative relationship between these two activities along the mung bean hypocotyl. Mung beans ( Vigna radiata L.) were selected because of the large body of information about other developmental gradients along its hypocotyl (7), and the relative ease of obtaining PM using aqueous twophase partitioning (21, 31). MATERIALS AND METHODS Plant MaterialMung bean (Vigna radiata L. cv Berken) seeds were obtained from Burpee Seed Co. Seeds were soaked with aeration overnight, then dark-grown at 25°C on soaked and drained coarse vermiculite. Three hundred milliliters ofa 1 mm CaSO4 solution were added to the vermiculite to prevent dampingoff symptoms. Hypocotyls were harvested after 5 d, when their lengths wer...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls.

Basel

Cleland²

1992

Plant Physiol.

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“…The hypothesis that its direct and primary action is at the plasma membrane, where it activates the W -ATPase responsible for electrogenic extrusion, which consequently in-fluences the activity of a number of metabolic and physiological processes, has received strong support by recent work (65). Its mode of action has been recently investigated by several groups.…”

Section: Structure and Mode Of Actionmentioning

confidence: 99%

Phytotoxins and Plant Pathogenesis

Graniti¹,

Durbin²,

Ballio³

1991

Cell to Cell Signals in Plants and Animals

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“…Structure of cercosporin. lemma H*-ATPase and to the elucidation of the involvement of membrane transport processes on cellular physiological respon.ses (Marre et al 1989). Another example is HMT-toxin, produced by race T isolates of Cochliobolus heterostrophus (formerly Helminthosporium maydis) the causal agent of southern corn leaf blight.…”

Section: Introductionmentioning

confidence: 99%

The photoactivated toxin cercosporin as a tool in fungal photobiology

Daub¹,

Ehrenshaft²

1993

Physiol Plant

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Daub. M. E. and Ehrenshaft, M. 1993. The photoactivated toxin cereosporin as a tool in fungal photobiology. -Physioi. Plant. 89: 227-236.Cercospora .species are a highly sucees.sful group of fungi which pathogenize diverse species of economically important plants. Many Cercospora species produce a unique photoaetivated and photoinduced polyketide toxin, eercosporin. which has been implicated as a pathogenicity factor. Illuminated cercosporin interacts with molecular oxygen to produce highly toxic singlet oxygen. Although nearly all organisms tested, including plants, mice and most fungi, are susceptible to cercosporin-mediated cell damage. Cercospora species are resistant. In general, little is known about how organisms protect themselves against singlet oxygen. Studies on how Cercospora species avoid autotoxicity are proving to be a valuable model in understanding this process in other systems. Furthermore, advances are being made in the understanding of how light regulates gene expression and cerco.sporin synthesis in Cercospora species. These studies are helping to elucidate mechanisms of gene regulation and light signal transduction for an environmental signal important in numerous fungal developmental processes, including secondary metabolite production.

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Action of Fusicoccin in vivo: Physiological and Biochemical Consequences

Cited by 6 publications

References 21 publications

Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls.

Comparison of developmental gradients for growth, ATPase, and fusicoccin-binding activity in mung bean hypocotyls.

Phytotoxins and Plant Pathogenesis

The photoactivated toxin cercosporin as a tool in fungal photobiology

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