Action of pentoxifylline on cytosolic calcium and on the erythrocytic morphology during ageing

Turchetti, V.; Matteis, C De; Leoncini, F.; Picciolini, F; Trabalzini, L.; Guerrini, M; Forconi, S

doi:10.3233/ch-1994-14605

Cited by 3 publications

(3 citation statements)

References 8 publications

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“…We have evaluated our method using RBCs. Not only are the morphologies 13 and deformabilities 14 of RBCs of great clinical interest but because RBCs are both readily available and their shapes and mechanical properties easily changed 15 they constitute an excellent model system for cells in general provided one keeps in mind that RBCs lack a nucleus and are therefore much simpler than most other cells.…”

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confidence: 99%

Sorting cells by size, shape and deformability

et al. 2012

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confidence: 99%

Sorting cells by size, shape and deformability

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“…In the case of mature or pre-mature twins, we measured ~10-12% of this type of phenotypical variant, with a clear indication of elevated oxidative stress. It has been documented that an increase in the formation of Burr cells population can be well-connected to some pathological events, like that of kidney failure, pyruvate kinase deficiency, and certain liver diseases [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Molecular Study on Twin Cohort with Discordant Birth Weight

2023

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The increased rate of twinning has pointed out newer challenges in clinical practices related to gestational complications, intrauterine growth restriction, perinatal mortality, and comorbidities. As a twin pregnancy progresses, the increased demand for oxygen supply can easily disrupt the redox homeostasis balance and further impose a greater challenge for the developing fetuses. A substantial birth-weight difference acts as an indicator of a deficit in oxygenation or blood flow to one of the fetuses, which might be related to a low bioavailable nitric oxide level. Therefore, in this study, we focused on networks involved in the adjustment of oxygen supply, like the activation of inducible and endothelial nitric oxide synthase (NOS3) along with free radical and lipid peroxide formation in mature twin pairs with high birth-weight differences. The selected parameters were followed by immunofluorescence staining, fluorescence-activated cell sorting analysis, and biochemical measurements in the umbilical cord vessels and fetal red blood cells. Based on our data set, it is clear that the lower-weight siblings are markedly exposed to persistent intrauterine hypoxic conditions, which are connected to a decreased level in NOS3 activation. Furthermore, the increased level of peroxynitrite aggravates lipid peroxidation and induces morphological and functional damage and loss in redox homeostasis.

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“…68 69 Relations between cytosolic calcium concentration and mechanical alterations have been reported in peripheral vascular diseases and exercise and as a result of drug therapy or hormone level abnormality. 70 71 72 73 74 It has been experimentally demonstrated that an increased calcium level rigidifies the cytoskeletal network, most likely through a calmodulin-dependent mechanism. 75 76 The exact site of this interaction is not clear, but spectrin-actin band 4.1 binding sites are considered to be involved.…”

Section: Clinical Aspects Of Blood Rheologymentioning

confidence: 99%

Blood Rheology and Hemodynamics

Baskurt,

Meiselman

2023

Semin Thromb Hemost

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Seminars in Thrombosis and Hemostasis (STH) celebrates 50 years of publishing in 2024. To celebrate this landmark event, STH is republishing some archival material. This manuscript represents the most highly cited paper ever published in STH. The original abstract follows.Blood is a two-phase suspension of formed elements (i.e., red blood cells [RBCs], white blood cells [WBCs], platelets) suspended in an aqueous solution of organic molecules, proteins, and salts called plasma. The apparent viscosity of blood depends on the existing shear forces (i.e., blood behaves as a non-Newtonian fluid) and is determined by hematocrit, plasma viscosity, RBC aggregation, and the mechanical properties of RBCs. RBCs are highly deformable, and this physical property significantly contributes to aiding blood flow both under bulk flow conditions and in the microcirculation. The tendency of RBCs to undergo reversible aggregation is an important determinant of apparent viscosity because the size of RBC aggregates is inversely proportional to the magnitude of shear forces; the aggregates are dispersed with increasing shear forces, then reform under low-flow or static conditions. RBC aggregation also affects the in vivo fluidity of blood, especially in the low-shear regions of the circulatory system. Blood rheology has been reported to be altered in various physiopathological processes: (1) Alterations of hematocrit significantly contribute to hemorheological variations in diseases and in certain extreme physiological conditions; (2) RBC deformability is sensitive to local and general homeostasis, with RBC deformability affected by alterations of the properties and associations of membrane skeletal proteins, the ratio of RBC membrane surface area to cell volume, cell morphology, and cytoplasmic viscosity. Such alterations may result from genetic disorders or may be induced by such factors as abnormal local tissue metabolism, oxidant stress, and activated leukocytes; and (3) RBC aggregation is mainly determined by plasma protein composition and surface properties of RBCs, with increased plasma concentrations of acute phase reactants in inflammatory disorders a common cause of increased RBC aggregation. In addition, RBC aggregation tendency can be modified by alterations of RBC surface properties because of RBC in vivo aging, oxygen-free radicals, or proteolytic enzymes. Impairment of blood fluidity may significantly affect tissue perfusion and result in functional deteriorations, especially if disease processes also disturb vascular properties.

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Action of pentoxifylline on cytosolic calcium and on the erythrocytic morphology during ageing

Cited by 3 publications

References 8 publications

Sorting cells by size, shape and deformability

Sorting cells by size, shape and deformability

Molecular Study on Twin Cohort with Discordant Birth Weight

Blood Rheology and Hemodynamics

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