Action of phospholipase C at the neuromuscular junction in rodent skeletal muscles

Harborne, Alan J.; Turner, A.; Murphy, Richard L.W.; Smith, Margaret E.

doi:10.1016/0014-2999(87)90529-2

Cited by 5 publications

(1 citation statement)

References 25 publications

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“…Phospholipases are enzymes that hydrolyze phospholipids into inositol phosphates and diacylglycerol, which are implicated as second messengers for the subsequent cellular events (Kadamur and Ross, 2013 ). Phospholipase C (PLC), one of the major classes of phospholipases, evokes endplate responses to acetylcholine in rodent skeletal muscle (Harborne et al, 1987 ). Two other classes of phospholipases are likely to have presynaptic roles.…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Huang

Ito

et al. 2020

Front. Mol. Neurosci.

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The neuromuscular junction (NMJ) is a prototypic chemical synapse between the spinal motor neuron and the motor endplate. Gene expression profiles of the motor endplate are not fully elucidated. Collagen Q (ColQ) is a collagenic tail subunit of asymmetric forms of acetylcholinesterase and is driven by two distinct promoters. pColQ1 is active throughout the slow-twitch muscle, whereas pColQ1a is active at the motor endplate of fast-twitch muscle. We made a transgenic mouse line that expresses nuclear localization signal (NLS)-attached Cre recombinase under the control of pColQ1a (pColQ1a-Cre mouse). RiboTag mouse expresses an HA-tagged ribosomal subunit, RPL22, in cells expressing Cre recombinase. We generated pColQ1a-Cre:RiboTag mouse, and confirmed that HA-tagged RPL22 was enriched at the NMJ of tibialis anterior (TA) muscle. Next, we confirmed that Chrne and Musk that are specifically expressed at the NMJ were indeed enriched in HA-immunoprecipitated (IP) RNA, whereas Sox10 and S100b, markers for Schwann cells, and Icam1, a marker for vascular endothelial cells, and Pax3, a marker for muscle satellite cells, were scarcely detected. Gene set enrichment analysis (GSEA) of RNA-seq data showed that "phosphatidylinositol signaling system" and "extracellular matrix receptor interaction" were enriched at the motor endplate. Subsequent analysis revealed that genes encoding diacylglycerol kinases, phosphatidylinositol kinases, phospholipases, integrins, and laminins were enriched at the motor endplate. We first characterized the gene expression profile under translation at the motor endplate of TA muscle using the RiboTag technique. We expect that our gene expression profiling will help elucidate molecular mechanisms of the development, maintenance, and pathology of the NMJ.

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Section: Discussionmentioning

confidence: 99%

Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Huang

Ito

et al. 2020

Front. Mol. Neurosci.

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Positive Modulators of Acetylcholine Receptor: Differences Between Skeletal Muscle and Electric Organ

Eterović

Hann

Ferchmin

et al. 1989

Molecular Biology of Neuroreceptors and Ion Channels

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Electric organ polyamines and their effects on the acetylcholine receptor

et al. 1992

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1. The electric organ of Torpedo nobiliana contained putrescine (PUT), spermidine (SPD), spermine (SPM), and cadaverine (CAD). Traces of acetylated SPD and SPM were occasionaly seen. 2. Upon fractionation of the tissue by differential centrifugation, the polyamines (PA) were found predominantly in the soluble fraction. The postsynaptic membrane fraction, containing a high concentration of acetylcholine receptor (AChR), was proportionally enriched in SPM. The molar ratio of SPM to AChR was approximately two in these membranes. 3. The effect of exogeneous PA on AChR function was studied by two methods: carbamoylcholine (CCh)-dependent 86Rb+ influx into receptor-rich membrane vesicles and [alpha-125I]bungarotoxin (Bgt) binding to the AChR. 4. SPM inhibited both ion influx and the rate of Bgt binding at concentrations above 1 mM, and therefore it appears to act as a competitive antagonist of the AChR. 5. At submicromolar concentrations, and only after preincubation with the receptor-rich membrane, SPM and PUT increased the ion influx by about 20% over control values. 6. Preincubation with 100 nM SPM did not affect the equilibrium binding of iodinated toxin or the rate of toxin binding, and therefore SPM was not uncovering new receptors. 7. By measuring the initial rate of toxin binding after different periods of preincubation with 1 microM CCh, the rate of the slow phase of receptor desensitization was determined. This rate was not changed by 100 nM SPM. 8. Although these results suggest that at low concentrations SPM is a positive modulator of the AChR, the precise mechanism of action is not determined yet.

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Action of phospholipase C at the neuromuscular junction in rodent skeletal muscles

Cited by 5 publications

References 25 publications

Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Gene Expression Profile at the Motor Endplate of the Neuromuscular Junction of Fast-Twitch Muscle

Positive Modulators of Acetylcholine Receptor: Differences Between Skeletal Muscle and Electric Organ

Electric organ polyamines and their effects on the acetylcholine receptor

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