Action of spontaneously produced beta interferon in differentiation of embryonal carcinoma cells through an autoinduction mechanism.

Belhumeur, Pierre; Lanoix, Joël; Blais, Yves; Forget, Diane; Steyaert, Alain; Skup, Daniel

doi:10.1128/mcb.13.5.2846

Cited by 26 publications

(23 citation statements)

References 69 publications

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“…28,30,31 This hypothesis is supported by pharmacokinetics study showing that the half -life of IFNin the circulation of humans is about 5 minutes. One hour after a bolus intravenous dose of 6Â10 6 units of IFN -, serum concentrations were less than 8 U /mL and after intramuscular or subcutaneous injection, they were less than 2 U /mL. 32 These concentrations are far below those required to suppress tumor cell growth, down -regulate angiogenesis, and activate macrophages, natural killer cells, and specific T-cell responses.…”

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confidence: 99%

Adenovirus-mediated interferon-β gene therapy suppresses growth and metastasis of human prostate cancer in nude mice

Cao

Wang

et al. 2001

Cancer Gene Ther

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The purpose of this study was to determine the effects of interferon -(IFN -) gene transfer on the growth of PC3MM2 human prostate cancer cells in nude mice. Intralesional delivery of an adenoviral vector encoding murine IFN -( AdIFN -) , but not a vector encoding bacterial -galactosidase ( AdLacZ ) , suppressed PC3MM2 tumors in a dose -dependent manner. At the highest dose ( 2Â10 9 plaque -forming units, PFU ) , a single injection of AdIFN -( but not AdLacZ ) suppressed orthotopic PC3MM2 tumors and development of metastasis by 80%, and eradicated the tumors in 20% of mice. Immunohistochemical staining showed that AdIFN -± treated tumors contained fewer microvessels, fewer proliferating cells, and more apoptotic cells than did the control tumors. Compared with controls, tumors injected with AdIFN -expressed higher levels of IFN -and inducible nitric oxide synthase ( iNOS ) and lower levels of basic fibroblast growth factor ( bFGF ) and transforming growth factor 1 ( TGF -1). In vitro analysis indicated that expression of bFGF and TGF -1 in PC3MM2 cells could be suppressed by the nitric oxide donor sodium nitroprusside. These data suggest that intratumoral delivery of the IFN -gene with adenoviral vectors could be an effective therapy for prostate cancer and that tumor suppression by AdIFN -correlated with up -regulation of iNOS and down -regulation of angiogenesis.

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confidence: 99%

Adenovirus-mediated interferon-β gene therapy suppresses growth and metastasis of human prostate cancer in nude mice

Cao

Wang

et al. 2001

Cancer Gene Ther

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“…The degree of dierentiation and the dierentiation potential of the various populations was evaluated using the colony dierentiation assay described by us and others (Belhumeur et al, 1993;Berg and McBurney, 1990). This simple and rapid method has been shown to accurately assess the dierentiation potential of stem cell populations; Belhumeur et al, 1993;Berg and McBurney, 1990).…”

Section: Transfection Of Dominant-negative Mutants Of Krox24 Blocks Dmentioning

confidence: 99%

“…This simple and rapid method has been shown to accurately assess the dierentiation potential of stem cell populations; Belhumeur et al, 1993;Berg and McBurney, 1990). The results are presented in Figure 9.…”

Section: Transfection Of Dominant-negative Mutants Of Krox24 Blocks Dmentioning

confidence: 99%

“…Treatment with RA in monolayer or by aggregation with RA or DMSO was also according to Rudnicki and McBurney (1987). The colony assay used to quantitate dierentiation capacity was performed as described by Belhumeur et al (1993). Essentially, 10 4 P19 cells were plated onto 6-cm-diameter petri dishes and allowed to adhere.…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

“…Values presented are averages of several experiments, done with triplicate counts of at least 1500 colonies per count. The validity of this simple method has been con®rmed by marker analysis (Belhumeur et al, 1993;Berg and McBurney, 1990).…”

Section: Cells and Culture Conditionsmentioning

confidence: 99%

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Wild-type egr1/Krox24 promotes and dominant-negative mutants inhibit, pluripotent differentiation of p19 embryonal carcinoma cells

Lanoix

Mullick

He³

et al. 1998

Oncogene

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A fission yeast RCC1-related protein is required for the mitosis to interphase transition.

1994

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The isolation and characterization of the mutant dcdts (defect in chromatin decondensation) has led to the identification of two conserved proteins required for the re‐establishment of the interphase state following the completion of mitosis. The gene that rescues the dcdts mutant encodes a protein similar to the human chromatin binding protein, RCC1. A suppressor of dcdts encodes a protein nearly identical to the human GTP‐binding protein, RAN, encoded by the TC4 gene. These results indicate that completion of mitosis is regulated at least in part by a GTPase molecular switch. The gene and suppressor of dcdts are identical to the previously described Schizosaccharomyces pombe genes pim1 (premature initiation of mitosis) and spi1 (suppressor of pim), but the dcdts mutant does not enter mitosis prematurely, a phenotype that has been reported for the pim1‐46ts mutant. Based on our studies we propose that the pim1 gene product is required for regulating chromatin condensation with a primary role at the end of mitosis and pleiotropic effects on other aspects of cell behavior.

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Action of spontaneously produced beta interferon in differentiation of embryonal carcinoma cells through an autoinduction mechanism.

Abstract: In the current study, we have addressed the role of interferons (IFNs)

Cited by 26 publications

References 69 publications

Adenovirus-mediated interferon-β gene therapy suppresses growth and metastasis of human prostate cancer in nude mice

Adenovirus-mediated interferon-β gene therapy suppresses growth and metastasis of human prostate cancer in nude mice

Wild-type egr1/Krox24 promotes and dominant-negative mutants inhibit, pluripotent differentiation of p19 embryonal carcinoma cells

A fission yeast RCC1-related protein is required for the mitosis to interphase transition.

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