Actions of calcium influx blockers in human neutrophils support a role for receptor-operated calcium entry

Salmon, Michael D.; Ahluwalia, Jatinder

doi:10.1016/j.cellimm.2010.02.001

Cited by 10 publications

(16 citation statements)

References 13 publications

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“…In a variety of cultured cell lines and endothelial cells, it has been demonstrated that ML-9 inhibits thapsigargin induced Ca 2+ release and hence SOCE [15][16][17][18]. Recently, using human neutrophils, we demonstrated that ML-9 inhibits Ca 2+ influx stimulated by thapsigargin but not by the agonists FMLP, PAF, or LTB 4 [7]. This observation provided evidence that MLCK was implicated in the signaling pathway associated with SOCE in human neutrophils.…”

Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 55%

“…The precise molecular target of ML-9 remains blurred, although there is evidence that ML-9 might be directly interacting with elements of SOCE namely STIM1 [18]. Although ML-9 inhibits thapsigargin stimulated Ca 2+ entry, pre-incubation with this inhibitor caused a marked enhancement in both agonist stimulated Ca 2+ release and entry in human neutrophils [7]. The augmentation might be due to ML-9 opening InsP 3 Ca 2+ release Fig.…”

Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 99%

“…Thapsigargin causes a slow depletion of Ca 2+ stores followed by massive influx leading to toxic [Ca 2+ ] i . In comparison, agonist mediated depletion of endoplasmic reticulum (ER) stores occurs much more rapidly than thapsigargin [7], followed by a more moderate Ca 2+ influx which is more physiologically relevant. We have recently provided evidence that receptor operated influx pathways may be important in neutrophils [7,8].…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

“…The piperazine compound ML-9 (1-(5-chloronaphthalene-1-sulfonyl) homopiperazine, HCl), has been shown to inhibit SOCE in monocytes and macrophages [12] but was only recently investigated in human neutrophils [7]. Originally, ML-9 was reported to potently inhibit myosin light-chain kinase (MLCK) by acting competitively at the binding site for ATP [13].…”

Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 99%

“…Indeed, perhaps one bona fide characteristic of SOCE is that it is inhibited by low concentrations of Gd 3+ [20]. In human neutrophils, this is without exception as 1 μM Gd 3+ completely abolishes thapsigargin stimulated Ca 2+ entry while only partially inhibiting agonist stimulated Ca 2+ entry at 1 μM [7]. Further studies would be required to determine the IC50 values for the partial inhibition of Ca 2+ entry mediated by agonists in human neutrophils.…”

Section: Gd 3+ Inhibits Soce But Not Roce In Human Neutrophilsmentioning

confidence: 99%

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Pharmacology of receptor operated calcium entry in human neutrophils

Salmon

Ahluwalia

2011

International Immunopharmacology

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Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 55%

Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 99%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Section: Ml-9 Reveals Differences Between Soce and Roce In Neutrophilsmentioning

confidence: 99%

Section: Gd 3+ Inhibits Soce But Not Roce In Human Neutrophilsmentioning

confidence: 99%

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Pharmacology of receptor operated calcium entry in human neutrophils

Salmon

Ahluwalia

2011

International Immunopharmacology

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Polychlorinated biphenyl 19 blocks the most common form of store-operated Ca2+ entry through Orai

Lee

Kim

Cho

et al. 2017

Naunyn-Schmiedeberg's Arch Pharmacol

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PCB19, a 2,2',6-trichlorinated biphenyl, is one of many non-dioxin-like polychlorinated biphenyls (NDL-PCBs), which are ubiquitous pollutants. NDL-PCBs affect cytosolic Ca signaling by promoting Ca release from ryanodine receptor-sensitive Ca pools and inhibiting store-operated Ca entry (SOCE) from the extracellular space. However, NDL-PCB-mediated SOCE inhibition has only been demonstrated in PC12 cells, in which SOCE is thought to be mainly mediated by TRPC family channels. Here, we investigated the effect of PCB19 on SOCE using human embryonic kidney 293 (HEK293) cells, human leukemia T cell line Jurkat-T cells and human promyelocytoma HL-60 cells which are the cell lines that are previously demonstrated to mediate the most common form of SOCE solely by the intrinsic Orai channels. PCB19 reduced thapsigargin-induced Ca influx after Ca pool depletion in HEK293 cells. SOCEs in HEK293, Jurkat T, HL-60 and PC12 cells showed distinct sensitivities to SOCE inhibitors such as Gd and ML-9; however, PCB19 also showed a common effect of inhibiting SOCEs in all cell lines. PCB19-mediated SOCE inhibition was confirmed by demonstrating the ability of PCB19 to inhibit the SOCE current but not the TRPM7 current. These results imply that PCB19 inhibits not only TRPC-mediated SOCE as in PC12 cells but also Orai-mediated SOCE as in many other cells including HEK293, Jurkat T and HL-60 cells.

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Effects of the semisynthetic bis-indole derivative KAR-2 on store-operated calcium entry in human neutrophils

Salerno

Capuozzò

2013

Archives of Biochemistry and Biophysics

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Actions of calcium influx blockers in human neutrophils support a role for receptor-operated calcium entry

Cited by 10 publications

References 13 publications

Pharmacology of receptor operated calcium entry in human neutrophils

Pharmacology of receptor operated calcium entry in human neutrophils

Polychlorinated biphenyl 19 blocks the most common form of store-operated Ca2+ entry through Orai

Effects of the semisynthetic bis-indole derivative KAR-2 on store-operated calcium entry in human neutrophils

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