Actions of juglone on energy metabolism in the rat liver

Saling, Simoni Cristina; Comar, Jurandir Fernando; Mito, Márcio Shigueaki; Peralta, Rosane Marina

doi:10.1016/j.taap.2011.09.004

Cited by 52 publications

(33 citation statements)

References 30 publications

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“…Juglone (5-hydroxy-1,4-naphthoquinone) is a natural quinine from Juglans regia with toxic pro-oxidant activity (Saling et al, 2011). Exposure of C. elegans to a high concentration of juglone kills the worms; however, antioxidant compounds can prevent such an effect.…”

Section: Resultsmentioning

confidence: 99%

Identification of phenolic secondary metabolites fromSchotia brachypetalaSond. (Fabaceae) and demonstration of their antioxidant activities inCaenorhabditis elegans

Sobeh

Elhawary

Peixoto

et al. 2016

PeerJ

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BackgroundSchotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied. The present work aimed at profiling the major phenolics compounds present in the hydro-alcohol extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods.MethodsIn vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C. elegans, Survival assay, GFP expression and Subcellular DAF-16 localization were employed to evaluate the antioxidant activity.ResultsMore than forty polyphenols, including flavonoid glycosides, galloylated flavonoid glycosides, isoflavones, dihydrochalcones, procyanidins, anthocyanins, hydroxy benzoic acid derivatives, hydrolysable tannins, and traces of methylated and acetylated flavonoid derivatives were identified. Three compounds were isolated and identified from the genus Schotia for the first time, namely gallic acid, myricetin-3-O-α-L-1C4-rhamnoside and quercetin-3-O-L-1C4-rhamnoside. The total phenolics content of SBE was (376 mg CAE/g), followed by flavonoids (67.87 QE/g). In vitro antioxidant activity of SBE was evidenced by DPPH radical scavenging activity (IC50 of 9 µg/mL), FRAP ferric reducing activity (5,000 mol Fe2+ E/mg) and ABTS peroxide inhibiting activity (1,054 mM Trolox E/mg). The tested extract was able to protect the worms against juglone induced oxidative stress, an increased survival rate (up to 41%) was recorded, when compared with the control group (11%) and attenuate the reactive oxygen species (ROS) accumulation in dose-dependent and reached up to 72% for the highest tested concentration. SBE was also able to attenuate the levels of heat shock protein (HSP) expression in dose-dependent up to 60% in the 150 µg SBE/mL group. In DAF-16 Subcellular localization SBE treated worms showed nuclear localization pattern up to 78%, while it was only 5% in the untreated control group.DiscussionA pronounced antioxidant activity in vivo, which can be attributed to its ability to promote the nuclear translocation of DAF-16/FOXO, the main transcription factor regulating the expression of stress response genes. The remarkable antioxidant activity in vitro and in vivo correlates to SBE rich phenolic profile.

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Section: Resultsmentioning

confidence: 99%

Identification of phenolic secondary metabolites fromSchotia brachypetalaSond. (Fabaceae) and demonstration of their antioxidant activities inCaenorhabditis elegans

Sobeh

Elhawary

Peixoto

et al. 2016

PeerJ

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“…Juglone is a phenolic compound used in popular medicine as a phytotherapic in treating inflammatory and infectious diseases (Saling et al, 2011). Juglone, a well-known walnut component, is found in considerable amounts in all green and growing parts of trees and unripe hulls of the fruit (Prasad, 2003).…”

Section: Resultsmentioning

confidence: 99%

Seasonal variation of the main individual phenolics and juglone in walnut (Juglans regia) leaves

Cosmulescu¹,

Trandafir

Nour³

2013

Pharmaceutical Biology

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“…36 Mitochondrial oxygen consumption was measured polarographically using a Teflon-shielded platinum electrode. 36 Mitochondrial oxygen consumption was measured polarographically using a Teflon-shielded platinum electrode.…”

Section: Mitochondrial Ros Production and Respirationmentioning

confidence: 99%

β‐Caryophyllene, the major constituent of copaiba oil, reduces systemic inflammation and oxidative stress in arthritic rats

Ames-Sibin

Barizão

Castro-Ghizoni

et al. 2018

J of Cellular Biochemistry

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The current study investigated the action of β-caryophyllene, the major constituent of copaiba oil, on the systemic inflammation, oxidative status, and liver cell metabolism of rats with adjuvant-induced arthritis, a model for rheumatoid arthritis. This study also compared the actions of β-caryophyllene with those previously reported for copaiba oil on arthritic rats. For this purpose, Holtzman healthy and arthritic rats received 215 and 430 mg·kg β-caryophyllene orally once a day during 18 days. Both doses of β-caryophyllene reduced the adjuvant-induced paw edema, swollen of lymph nodes, and number of circulating and articular leukocytes. β-Caryophyllene, at the dose of 430 mg·kg , abolished the increases of protein carbonyl groups and myeloperoxidase activity in the liver and plasma of arthritic rats and, at both doses, it restored the increased levels of reactive oxygen species and reduced glutathione in the arthritic liver. These beneficial actions were of the same extension as those of copaiba oil ( Copaifera reticulata) and, therefore, β-caryophyllene is possibly responsible for the anti-inflammatory and antioxidant actions of the oil. Hepatic gluconeogenesis was 40% lower in arthritic rats, which also presented a reduced number of hepatocytes per liver area (-23%) associated with increased hepatocyte area (+18%) and liver weight (+50%). None of these hepatic alterations were improved by β-caryophyllene, but not even by ibuprofen. However, unlike copaiba oil, β-caryophyllene did not modify the hepatic morphology and metabolism of healthy rats. These results reveal that β-caryophyllene improves the systemic inflammation and oxidative status of arthritic rats and, in addition, it was not associated with hepatotoxicity.

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Actions of juglone on energy metabolism in the rat liver

Cited by 52 publications

References 30 publications

Identification of phenolic secondary metabolites fromSchotia brachypetalaSond. (Fabaceae) and demonstration of their antioxidant activities inCaenorhabditis elegans

Identification of phenolic secondary metabolites fromSchotia brachypetalaSond. (Fabaceae) and demonstration of their antioxidant activities inCaenorhabditis elegans

Seasonal variation of the main individual phenolics and juglone in walnut (Juglans regia) leaves

β‐Caryophyllene, the major constituent of copaiba oil, reduces systemic inflammation and oxidative stress in arthritic rats

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