Activatable Fluorescence/MRI Bimodal Platform for Tumor Cell Imaging via MnO<sub>2</sub> Nanosheet–Aptamer Nanoprobe

Zhao, Zilong; Fan, Huanhuan; Zhou, Gaofeng; Bai, Huarong; Liang, Hao; Wang, Ruowen; Zhang, Xiaobing; Tan, Weihong

doi:10.1021/ja5029364

Cited by 538 publications

(387 citation statements)

References 37 publications

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“…2,3 Other emerging nanosheets (for example, MoS 2 , WS 2 and MnO 2 ) with a good fluorescence-quenching ability also exhibit selective adsorption affinity toward single-stranded DNA (ssDNA) versus double-stranded DNA (dsDNA). [4][5][6][7] These results have inspired studies to exploit the potential biological applications of novel 2D nanosheets. Very recently, 2D metal-organic framework (MOF) thin films or nanosheets have been successfully synthesized and exfoliated by several groups.…”

Section: Introductionmentioning

confidence: 99%

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Wang

et al. 2017

NPG Asia Mater

166

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Two-dimensional (2D) materials have attracted tremendous interest as fluorescence quenchers of dye-labeled biomolecules for application in biosensing. Metal-organic framework (MOF) nanosheets, as a new type of 2D material, have rarely been studied as bioanalytical platforms. Herein, we synthesize a series of ultrathin lanthanide-based MOF (MOF-Ln) nanosheets as a dye-labeled aptamer platform. The fluorescence quenching or recovery on the MOF-Ln nanosheets is determined by the charge properties (positive or negative) of the labeled fluorophores. The negatively charged fluorophores experience a fluorescence 'turn-down followed by turn-down' process, whereas the positively charged fluorophores experience a fluorescence 'turn-down followed by turn-up' process. The interesting fluorescence quenching properties of the MOF-Ln nanosheets make them an excellent two-color sensing platform for the intracellular detection of biomolecules. NPG Asia Materials (2017) 9, e354; doi:10.1038/am.2017.7; published online 10 March 2017 INTRODUCTION Two-dimensional (2D) nanomaterials have been attracting extensive research interest due to their unique physical and chemical properties, as well as their potential scientific and technological applications in the fields of gas storage, sensing, electronics, energy conversion and storage, and electrocatalysis. 1 In the area of biomedical applications, 2D nanosheets with an extremely high surface area, such as graphene and its derivative graphene oxide, have been successfully used for biomedical imaging, drug delivery and cancer therapy. 2,3 Other emerging nanosheets (for example, MoS 2 , WS 2 and MnO 2 ) with a good fluorescence-quenching ability also exhibit selective adsorption affinity toward single-stranded DNA (ssDNA) versus double-stranded DNA (dsDNA). [4][5][6][7] These results have inspired studies to exploit the potential biological applications of novel 2D nanosheets. Very recently, 2D metal-organic framework (MOF) thin films or nanosheets have been successfully synthesized and exfoliated by several groups. [8][9][10] Their potential biological applications remain to be explored.MOFs are a fascinating class of functional materials that have been extensively studied for applications in gas storage, 11 catalysis, 12 separation 13 and sensing. 14 MOFs are certainly very promising for fabricating multifunctional luminescent sensors, because both the metal and the ligand units can provide platforms for generating luminescence and some guest molecules loaded on the MOFs can also emit or induce luminescence. 14 A variety of MOFs have been already

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Section: Introductionmentioning

confidence: 99%

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Wang

et al. 2017

NPG Asia Mater

166

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“…The sensing mechanism of the above FRET assays were all based on the selective reduction of MnO 2 to Mn 2+ ions, which could recover the fluorescence signals of nanoparticle donors. nanosheet−aptamer nanoprobe (Zhao et al, 2014b). In this platform, the MRI imaging was realized by the large amount of paramagnetic Mn 2+ ions reduced by glutathione from MnO 2 .…”

Section: Tmd Nanosheets Based Fret Assaysmentioning

confidence: 99%

“…Graphene-like 2D materials such as TMDs (MnO 2 , MoS 2 and WS 2 ) have been used as good quenchers for nucleic acid based detection Zhao et al, 2014b;Zhu et al, 2013;Xi et al, 2014). Generally, fluorophore labelled nucleic acid was adsorbed and quenched on the basal planes of graphene-like 2D materials.…”

Section: Ws 2 Based Fret Assaysmentioning

confidence: 99%

Graphene and graphene-like two-denominational materials based fluorescence resonance energy transfer (FRET) assays for biological applications

Tian

Lyu

Shi

et al. 2017

Biosensors and Bioelectronics

159

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“…[11] First, MnO 2 nanosheets can strongly adsorb ssDNA by physisorption between nucleobases and MnO 2 nanosheets, which can facilitate the endocytosis of ssDNA. Second, MnO 2 nanosheets have an intense and broad optical absorption spectrum (l % 200-600 nm), making them an efficient broad-spectrum fluorescence quencher.…”

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confidence: 99%

A Smart DNAzyme–MnO₂ Nanosystem for Efficient Gene Silencing

et al. 2015

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DNAzymes hold promise for gene-silencing therapy, but the lack of sufficient cofactors in the cell cytoplasm, poor membrane permeability, and poor biostability have limited the use of DNAzymes in therapeutics. We report a DNAzyme-MnO2 nanosystem for gene-silencing therapy. MnO2 nanosheets adsorb chlorin e6-labelled DNAzymes (Ce6), protect them from enzymatic digestion, and efficiently deliver them into cells. The nanosystem can also inhibit (1)O2 generation by Ce6 in the circulatory system. In the presence of intracellular glutathione (GSH), MnO2 is reduced to Mn(2+) ions, which serve as cofactors of 10-23 DNAzyme for gene silencing. The release of Ce6 generates (1)O2 for more efficient photodynamic therapy. The Mn(2+) ions also enhance magnetic resonance contrast, providing GSH-activated magnetic resonance imaging (MRI) of tumor cells. The integration of fluorescence recovery and MRI activation provides fluorescence/MRI bimodality for monitoring the delivery of DNAzymes.

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Activatable Fluorescence/MRI Bimodal Platform for Tumor Cell Imaging via MnO₂ Nanosheet–Aptamer Nanoprobe

Cited by 538 publications

References 37 publications

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Graphene and graphene-like two-denominational materials based fluorescence resonance energy transfer (FRET) assays for biological applications

A Smart DNAzyme–MnO₂ Nanosystem for Efficient Gene Silencing

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Activatable Fluorescence/MRI Bimodal Platform for Tumor Cell Imaging via MnO2 Nanosheet–Aptamer Nanoprobe

Cited by 538 publications

References 37 publications

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Lanthanide-based metal-organic framework nanosheets with unique fluorescence quenching properties for two-color intracellular adenosine imaging in living cells

Graphene and graphene-like two-denominational materials based fluorescence resonance energy transfer (FRET) assays for biological applications

A Smart DNAzyme–MnO2 Nanosystem for Efficient Gene Silencing

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Activatable Fluorescence/MRI Bimodal Platform for Tumor Cell Imaging via MnO₂ Nanosheet–Aptamer Nanoprobe

A Smart DNAzyme–MnO₂ Nanosystem for Efficient Gene Silencing