Graphene and graphene-like two-denominational materials based fluorescence resonance energy transfer (FRET) assays for biological applications

Tian, Feng; Lyu, Jing; Shi, Jingyu; Yang, Mo

doi:10.1016/j.bios.2016.06.046

Cited by 160 publications

(56 citation statements)

References 136 publications

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“…4c, 4d shows the recovering of the fluorescence quenched by MWCNTs and GO, respectively. Remarkable recovery of the fluorescence quenched appeared for both of them, which was attributed to FRET generated by the synergy of particles collision and π-π stacking interaction between them and aptamer [22]. However, the recovery level varies.…”

Section: Resultsmentioning

confidence: 99%

Rapid Detection of Norovirus Using Paper-based Microfluidic Device

Weng

Neethirajan

2017

Preprint

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23Noroviruses (NoV) are the leading cause of outbreak of acute gastroenteritis worldwide. A 24 substantial effort has been made in the development of analytical devices for rapid and sensitive 25 food safety monitoring via the detection of foodborne bacteria, viruses and parasites. 26Conventional analytical approaches for noroviruses suffer from some critical weaknesses: labor-27 intensive, time-consuming, and relatively low sensitivity. In this study, we developed a rapid 28 and highly sensitive biosensor towards point-of-care device for noroviruses based on 6-29 carboxyfluorescein (6-FAM) labeled aptamer and nanomaterials, multi-walled carbon nanotubes 30 (MWCNTs) and graphene oxide (GO). In an assay, the fluorescence of 6-FAM labeled aptamer 31 was quenched by MWCNTs or GO via fluorescence resonance energy transfer (FRET). In the 32 presence of norovirus, the fluorescence would be recovered due to the release of the 6-FAM 33 labeled aptamer from MWCNTs or GO. An easy-to-make paper-based microfluidic platform 34 made by nitrocellulose membrane was used to conduct the assay. The quantitative detection 35 of norovirus virus-like particles (NoV VLPs) was successfully performed. A linear range of 0-36 12.9 µg/mL with a detection limit of 40 pM and 30 pM was achieved for the MWCNTs and GO 37 based paper sensors, respectively. The results suggested the developed paper-based microfluidic 38 device is simple, cost-effective and holds the potential of rapid in situ visual determination for 39 noroviruses with remarkable sensitivity and specificity, which provides a new way for early 40 identification of NoV and thereby an early intervention for preventing the spread of an outbreak. 41

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Section: Resultsmentioning

confidence: 99%

Rapid Detection of Norovirus Using Paper-based Microfluidic Device

Weng

Neethirajan

2017

Preprint

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“…19,20 Recent studies have demonstrated that graphene quantum dots (GQDs) might be an ideal candidate. [21][22][23] Different from conventional nanosized drug platforms, GQDs exhibit extraordinary drug loading capacity via strong π-π stacking interactions and functional modification. 21 Notably, functionalized GQDs are assumed to have a single atomic layer and dimensions ,20 nm.…”

Section: Introductionmentioning

confidence: 99%

“…[21][22][23] Different from conventional nanosized drug platforms, GQDs exhibit extraordinary drug loading capacity via strong π-π stacking interactions and functional modification. 21 Notably, functionalized GQDs are assumed to have a single atomic layer and dimensions ,20 nm. 24,25 Even with the loading of traditional anticancer drugs, such as CDDP, they can still be prepared in nano-sizes, enabling them to easily pass through the nuclear pore complex and reside within the cells, and that might help combat hypoxia-induced drug efflux and chemoresistance.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, GQDs are made from carbon ensuring good water solubility, extraordinary biocompatibility, minimal toxicity, and stable fluorescence. 21,23,26,27 In this study, we developed a tumor-targeted polyethylene glycol-graphene quantum dots-Pt (GPt) nanocomposite, based on polyethylene glycol (PEG)-modified GQDs to carry Pt. It was demonstrated that GPt could dramatically improve the chemotherapeutic efficacy for OSCC in vitro and in vivo, which might serve as a useful method for future tumor-targeted treatment.…”

Section: Introductionmentioning

confidence: 99%

“…Graphene-like materials are preeminent multifunctional materials in biomedicine because of their outstanding characteristics, including biosecurity, and excellent thermal properties. 21 Unfortunately, the large lateral size of GO and the interactions among GO layers could cause GO accretion. 18 To avoid accretion of GO, GQDs were used to ensure that the nanocomposite became more stable and thus could be stored for weeks before use.…”

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confidence: 99%

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Antitumor effect of a Pt-loaded nanocomposite based on graphene quantum dots combats hypoxia-induced chemoresistance of oral squamous cell carcinoma

Zheng¹,

Yin²,

Cai³

et al. 2018

IJN

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Background Tumor microenvironment plays an important role in the chemoresistance of oral squamous cell carcinoma (OSCC). Hypoxia in the microenvironment is one of the important factors that contributes to OSCC chemoresistance; therefore overcoming hypoxia-mediated chemoresistance is one of the great challenges in clinical practice. Methods In this study, we developed a drug delivery system based on Pt-loaded, polyethylene glycol-modified graphene quantum dots via chemical oxidation and covalent reaction. Results Our results show that synthesized polyethylene glycol-graphene quantum dots-Pt (GPt) is about 5 nm in diameter. GPt sensitizes OSCC cells to its treatment in both normoxia and hypoxia conditions. Inductively coupled plasma-mass spectrometry assay shows that GPt enhances Pt accumulation in cells, which leads to a notable increase of S phase cell cycle arrest and apoptosis of OSCC cells in both normoxia and hypoxic conditions. Finally, compared with free cisplatin, GPt exhibits a strong inhibitory effect on the tumor growth with less systemic drug toxicity in an OSCC xenograft mouse tumor model. Conclusion Taken together, our results show that GPt demonstrates superiority in combating hypoxia-induced chemoresistance. It might serve as a novel strategy for future microenvironment-targeted cancer therapy.

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