Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells

Edelblute, Chelsea; Heller, Loree C.; Malik, Muhammad Arif; Heller, Richard

doi:10.1002/bit.25660

Cited by 24 publications

(22 citation statements)

References 45 publications

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“…Chelsea et al . recently reported that using the plasma-activated air could deliver plasmid DNA into cells29, also suggesting that ROS are important for plasma transfection as the electric field is not involved in this process. However, how do the extracellular ROS produced by plasma transport into cells needs to be concerned.…”

Section: Discussionmentioning

confidence: 99%

“…Examples of its translation into major healthcare innovations include tissue ablation and coagulation, disinfection of food and living tissues, wound healing, and even cancer treatment23242526. Several groups have reported that plasma jet with pulsed high frequency could increase the cell permeability and delivery of GFP vectors in mammalian cells such as HeLa cells, CHL cells, Jurkat cells, Hacat cells and MCF-7 cells272829. Leduc et al .…”

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confidence: 99%

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Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

Wang

et al. 2016

Sci Rep

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This study reports the potential of cold atmospheric plasma (CAP) as a versatile tool for delivering oligonucleotides into mammalian cells. Compared to lipofection and electroporation methods, plasma transfection showed a better uptake efficiency and less cell death in the transfection of oligonucleotides. We demonstrated that the level of extracellular aqueous reactive oxygen species (ROS) produced by gas plasma is correlated with the uptake efficiency and that this is achieved through an increase of intracellular ROS levels and the resulting increase in cell membrane permeability. This finding was supported by the use of ROS scavengers, which reduced CAP-based uptake efficiency. In addition, we found that cold atmospheric plasma could transfer oligonucleotides such as siRNA and miRNA into cells even in 3D cultures, thus suggesting the potential for unique applications of CAP beyond those provided by standard transfection techniques. Together, our results suggest that cold plasma might provide an efficient technique for the delivery of siRNA and miRNA in 2D and 3D culture models.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

Wang

et al. 2016

Sci Rep

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“…We previously reported the use of PAA for DNA transfer in vitro . 40 Initially, recellularized human dermal constructs were employed as an alternative delivery substrate before moving to an animal model. The recellularized dermal constructs provided a 3D structure more akin to human skin, and superior to existing in vitro skin models.…”

Section: Discussionmentioning

confidence: 99%

“…We previously confirmed the presence of ozone and NO 2 in the plasma-activated gas under operating parameters. 40 It is probable that the generation of diluted reactive species in the PAA assists plasmid DNA delivery. Both ozone and NO 2 effect cellular membranes, and the effects are enhanced with increasing product amounts.…”

Section: Discussionmentioning

confidence: 99%

“…We previously demonstrated utilizing PAA as a DNA transfer method in vitro . 40 Recellularized human dermis was used as a preliminary substrate before progressing to a mouse model. For in vivo testing, an animal model using BALB/c mice, a strain typically used for vaccine testing, was employed to determine the potential use of PAA as a noncontact DNA transfer method to a living tissue.…”

Section: Introductionmentioning

confidence: 99%

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Plasma-activated air mediates plasmid DNA delivery in vivo

Edelblute

Heller

Malik

et al. 2016

Molecular Therapy - Methods & Clinical Development

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Plasma-activated air (PAA) provides a noncontact DNA transfer platform. In the current study, PAA was used for the delivery of plasmid DNA in a 3D human skin model, as well as in vivo. Delivery of plasmid DNA encoding luciferase to recellularized dermal constructs was enhanced, resulting in a fourfold increase in luciferase expression over 120 hours compared to injection only (P < 0.05). Delivery of plasmid DNA encoding green fluorescent protein (GFP) was confirmed in the epidermal layers of the construct. In vivo experiments were performed in BALB/c mice, with skin as the delivery target. PAA exposure significantly enhanced luciferase expression levels 460-fold in exposed sites compared to levels obtained from the injection of plasmid DNA alone (P < 0.001). Expression levels were enhanced when the plasma reactor was positioned more distant from the injection site. Delivery of plasmid DNA encoding GFP to mouse skin was confirmed by immunostaining, where a 3-minute exposure at a 10 mm distance displayed delivery distribution deep within the dermal layers compared to an exposure at 3 mm where GFP expression was localized within the epidermis. Our findings suggest PAA-mediated delivery warrants further exploration as an alternative approach for DNA transfer for skin targets.

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Innovative Precision Gene‐Editing Tools in Personalized Cancer Medicine

et al. 2020

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The development of clustered regularly interspaced short palindromic repeats (CRISPR) has spurred a successive wave of genome‐engineering following zinc finger nucleases and transcription activator‐like effector nucleases, and made gene‐editing a promising strategy in the prevention and treatment of genetic diseases. However, gene‐editing is not widely adopted in clinics due to some technical issues that challenge its safety and efficacy, and the lack of appropriate clinical regulations allowing them to advance toward improved human health without impinging on human ethics. By systematically examining the oncological applications of gene‐editing tools and critical factors challenging their medical translation, genome‐editing has substantial contributions to cancer driver gene discovery, tumor cell epigenome normalization, targeted delivery, cancer animal model establishment, and cancer immunotherapy and prevention in clinics. Gene‐editing tools, epitomized by CRISPR, are predicted to represent a promising strategy toward the precise control of cancer initiation and development. However, some technical problems and ethical concerns are serious issues that need to be appropriately addressed before CRISPR can be incorporated into the next generation of molecular precision medicine. In this light, new technical developments to limit off‐target effects are discussed herein, and the use of gene‐editing approaches for treating otherwise incurable cancers is brought into focus.

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Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells

Cited by 24 publications

References 45 publications

Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

Intracellular ROS mediates gas plasma-facilitated cellular transfection in 2D and 3D cultures

Plasma-activated air mediates plasmid DNA delivery in vivo

Innovative Precision Gene‐Editing Tools in Personalized Cancer Medicine

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