Activation and regulation of primary metabolism during seed germination

Rosental, Leah; Nonogaki, Hiroyuki; Fait, Aaron

doi:10.1017/s0960258513000391

Cited by 172 publications

(158 citation statements)

References 116 publications

(257 reference statements)

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“…Attention should be given to the fact that the germination speed and the seedling growth rate were inversely proportional to the content of CO 2 released, considering that the simple correlation coefficients were -0.92 and -0.88 (p < 0.05), respectively, which corroborates with those reported by Rosental et al (2014), whose respiratory activity is indicative of vigor and its reduction directly affects the speed and growth rate of the seedlings. The high coefficients of correlation between the concentration of CO 2 obtained with the percentage of emerged seedlings suggest that the methodology was efficient in the prediction of the establishment of seedlings in the field, however, temperatures lower than or equal to 25 o C allow the greater separation of the lots, resulting in greater sensitivity in detecting differences in physiological potential in Triticum aestevium seeds not detected by viability tests, therefore, with the potential to classify lots according to their performance potential.…”

Section: Lotsupporting

confidence: 85%

“…The intense respiratory activity in the first hours of imbibition is necessary for the generation of the redox state (Rosental et al, 2014) and the energetic requirement is directed to the production of systems of removers of reactive forms of oxygen in seeds of lower physiological potential submitted to supra-optimal temperatures (Dantas et al, 2015).…”

Section: Determination Of Carbon Dioxide Concentrationmentioning

confidence: 99%

“…Vigor tests have been used in tools used by the seed industry to eliminate low-quality seed lots for commercialization. Respiratory activity is one of the first biological manifestations of loss of vigor (Rosental et al, 2014) and its detection may help in the monitoring and control of the quality of seed production by the seed industries.…”

Section: Introductionmentioning

confidence: 99%

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Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

et al. 2017

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RESUMO Submitted on February 29th, 2016 and accepted on August 22nd, 2017The objective of this study was to develop and validate the methodology of carbon dioxide concentration in the evaluation of vigor of Triticum aestivum L. seeds based on infrared spectroscopy. The proposed method quantifies CO 2 content using a drag system and exhaustion to the gas released by the seeds. Samples of 50 seeds of six lots of cultivar CD 123 were incubated at temperatures of 15, 25 and 40 °C. The CO 2 content released after 1, 3, 6, 9, 12 and 24 h of incubation were quantified. Additionally, the percentage of normal seedlings emerged in the field were evaluated. The simple correlation coefficients were evaluated among tests. After determining the reading conditions, the precision and accuracy of the proposed method were evaluated, using 15 lots of seeds. For the evaluation of the respiratory activity in Triticum aestivum seeds, a sample of 25 seeds, incubated at 25 °C for a minimum of 12 h is recommended since it allows to classify lots with different levels of vigor and predict the establishment of seedlings in the field, being the appropriate method for measuring CO 2 as it externalizes precision between successive measurements and agreement with the reference method.

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Section: Lotsupporting

confidence: 85%

Section: Determination Of Carbon Dioxide Concentrationmentioning

confidence: 99%

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Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

et al. 2017

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“…Several authors have indicated that the increase in AA concentration during germination is due to protein degradation, which results in the release of AA. The reduction in the AA concentration, typically at 3 and 5 das in these three species, has also been observed in Arabidopsis thaliana seeds after the application of a scarification treatment at 4 °C and in Lens culinaris and Pisum sativum seeds (Joosen et al 2013, Rosental et al 2014. In other species, an increase in the AA concentration during germination has also been observed (Kuo et al 2004).…”

Section: Mobilization Of Reservesmentioning

confidence: 61%

Comparison of pre-germination treatments in <em>Lupinus</em> spp. and their effects on germination and related solutes

et al. 2017

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Background: Physical dormancy in seeds of the genus Lupinus restricts their ecological or agricultural use. Hypothesis: This dormancy can be broken when seeds are subjected to physical and chemical pretreatments that increase germination, mobilize reserves and decrease ABA. Studied species: Seeds of Lupinus exaltatus (Le), L. campestris (Lc) and L. montanus (Lm) from Puebla, México. Methods:The following seven treatments, including a control, were applied: PG1 = 98 % H 2 SO 4 for 15 min, PG2 = wet sand at 80 °C for 5 min, PG3 = wet sand at 35 °C for 8 h and 16 h at 25 °C, PG4 = dry sand at 80 °C for 7 min, PG5 = dry sand at 150 °C for 1 min, PG6 = H 2 0 at 80 °C for 1 min, Control = untreated seeds. On days 0, 3, 5, 10 and 15 after seeding, we evaluated the percentage and rate of germination (GP and GR, respectively) and biochemical changes. Results: PG6 produced a higher GP in Le (41 %) and Lc (69 %), and PG1 produced a higher GP in Lm (37 %). In all three species, the highest GR was obtained with PG1 (1.95, 2.27 and 2.22 day -1 seeds, respectively). PG6 increased the protein concentration (53, 17, and 43 % for Le, Lc and Lm, respectively), amino acids (19, 44 and 31 %, respectively), reducing sugars (63, 18 and 96 %, respectively) and polyphenols (32, 55 and 75 %, respectively) but decreased soluble sugars (22, 29 and 23 %, respectively) and ABA relative to the control. Although only the correlation between the GP and amino acid content was significant and positive, and the correlation between GP, GR and soluble sugars was significant and negative. Conclusions: The effect of pre-germination treatments on germination was species-specific. The pretreatment with H 2 0 at 80 °C for 1 min increased germination and mobilized the seed's reserves in the process. Key words: Lupinus, physical dormancy, germination, protein, sugars. ResumenAntecedentes: La latencia física en semillas del género Lupinus restringe su uso ecológico o agronómico. Hipótesis: Esta latencia puede liberarse al aplicarles pre-tratamientos físicos y químicos, lo que incrementa la germinación, moviliza reservas y disminuye el ABA. Resultados: El PG6 promovió mayor PG en Le (41 %) y Lc (69 %), y el PG1 en Lm (37 %). En las tres especies, la mayor VG fue con el PG1 (1.95, 2.27 y 2.22 semillas día -1 , respectivamente). Con PG6, se observó un incremento en concentración de proteína (53, 17, 43 % para Le, Lc y Lm, respectivamente), aminoácidos (19, 44 y 31 %), azú-cares reductores (63, 18 y 96 %) y polifenoles (32, 55 y 75 %) pero disminuyeron los azúcares solubles (22, 29 y 23 %) y ABA respecto al control. Aunque solo la correlación entre PG y aminoácidos fue significativa y positiva y entre PG, VG y azúcares solubles es significativa y negativa. Conclusiones: Entre especies, el efecto de los tratamientos pre-germinativos sobre la germinación fue diferencial. El uso de H 2 0 80 °C 1 min incrementó germinación y movilizó las reservas durante el proceso.

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“…This observation corresponded with the finding that hydrolysis of starch reserves in the cotyledons commences after germination (Bewley et al 2013). The degradation of storage reserves including starch (and proteins) can contribute to the embryo growth potential (Rosental et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

Eliášová¹,

Pešek²,

Vondráková³

2015

Dendrobiology

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Understanding the mechanisms of seed dormancy is essential for planning optimal seed storage and for breaking the dormancy of stored seeds. Our experiments focused on three aspects of characterization of breaking the dormancy of Fagus sylvatica seeds during moist low temperature stratification. 1) We used the changes of ABA content in embryos during the whole process of stratification as the marker of the depth of dormancy: the decrease in the depth of dormancy (according standard germination tests) was directly proportional to the drop in endogenous ABA content in both cotyledons and embryonic axes. 2) Fumarase activity (the second marker of dormancy) increased gradually during stratification.3) The histochemical visualisation of storage compounds as the third marker of dormancy documented the changes in storage protein deposition during stratification. The proteinaceous content of storage vacuoles disappeared; the vacuoles diminished, petered out or fused, creating central vacuoles. This process commenced with imbibition of the seeds and it was more pronounced in the cells in the external parts of the cotyledons. No changes in the size and location of starch grains or calcium oxalate crystals linked with stratification were detected.

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Activation and regulation of primary metabolism during seed germination

Cited by 172 publications

References 116 publications

Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

Carbon dioxide quantified by the infrared in evaluation of respiratory activity of wheat seeds

Comparison of pre-germination treatments in <em>Lupinus</em> spp. and their effects on germination and related solutes

Storage compounds, ABA and fumarase in Fagus sylvatica embryos during stratification

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