Activation de la coagulation par la greffe intraportale d’îlots de Langerhans chez le porc*1Communication présentée à l’Académie nationale de chirurgie au cours de la séance du 16 mai 2001.

Lamblin, Antoine; Tournoys, Antoine; Gmyr, Valéry; Jourdain, M.; Lefèbvre, J; Kerr–Conte, Julie; Proye, C; Pattou, F.

doi:10.1016/s0003-3944(01)00594-6

Cited by 8 publications

(2 citation statements)

References 13 publications

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“…This initial application of the dual transplant model was utilized to directly compare IBMIR amongst intraportally infused GTKO and WT NPIs at 1 and 24 hour time intervals without immunosuppression. To segregate islet-specific inflammatory events from those associated with the mechanical effects of portal vein embolization, biologically inert material control consisting of polyethylene microspheres (MS) were also studied (33). …”

Section: Introductionmentioning

confidence: 99%

Dual Islet Transplantation Modeling of the Instant Blood-Mediated Inflammatory Reaction

Martin

Samy

et al. 2015

American Journal of Transplantation

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Islet xenotransplantation is a potential treatment for diabetes without the limitations of tissue availability. Although successful experimentally, early islet loss remains substantial and attributed to an instant blood mediated inflammatory reaction (IBMIR). This syndrome of islet destruction has been incompletely defined and characterization in pig-to-primate models has been hampered by logistical and statistical limitations of large animal studies. To further investigate IBMIR, we developed a novel in vivo dual islet transplant model to precisely characterize IBMIR as proof-of-concept that this model can serve to properly control experiments comparing modified xenoislet preparations. Wild-type (WT) and α1,3-galactosyltransferase knockout (GTKO) neonatal porcine islets (NPIs) were studied in non-immunosuppressed rhesus macaques. Inert polyethylene microspheres served as a control for the effects of portal embolization. Digital analysis of immunohistochemistry targeting IBMIR mediators was performed at one and 24 hours after intraportal islet infusion. Early findings observed in transplanted islets include complement and antibody deposition, and infiltration by neutrophils, macrophages, and platelets. Insulin, complement, antibody, neutrophils, macrophages, and platelets were similar between GTKO and WT islets, with increasing macrophage infiltration at 24 hours in both phenotypes. This model provides an objective and internally controlled study of distinct islet preparations and documents the temporal histology of IBMIR.

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Section: Introductionmentioning

confidence: 99%

Dual Islet Transplantation Modeling of the Instant Blood-Mediated Inflammatory Reaction

Martin

Samy

et al. 2015

American Journal of Transplantation

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“…The occurrence of thromboinflammation in vivo was originally demonstrated in the allogeneic pig models of intraportal islet transplantation (Bennet et al, 1999;Lamblin et al, 2001). Allogeneic IBMIR occurring during clinical islet transplantation was also established in patients who had undergone clinical islet transplantation (Johansson et al, 2005;Moberg et al, 2002b).…”

Section: The Instant Blood-mediated Inflammatory Reaction (Ibmir) In mentioning

confidence: 99%

The role and regulation of complement activation as part of the thromboinflammation elicited in cell therapies

Nilsson

Teramura

Ekdahl

2014

Molecular Immunology

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Human Fetal Islet Transplantation in Type 1 Diabetics: Comparison of Immunological Effects Between Multiple Implantation Regimens

Djordjević¹,

Lalić²,

Bumbaširević³

et al. 2005

Transplantation Proceedings

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Activation de la coagulation par la greffe intraportale d’îlots de Langerhans chez le porc*1Communication présentée à l’Académie nationale de chirurgie au cours de la séance du 16 mai 2001.

Cited by 8 publications

References 13 publications

Dual Islet Transplantation Modeling of the Instant Blood-Mediated Inflammatory Reaction

Dual Islet Transplantation Modeling of the Instant Blood-Mediated Inflammatory Reaction

The role and regulation of complement activation as part of the thromboinflammation elicited in cell therapies

Human Fetal Islet Transplantation in Type 1 Diabetics: Comparison of Immunological Effects Between Multiple Implantation Regimens

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