Stretch-attend posture (SAP) occurs during risk assessment and is prevalent in common rodent behavioral tests. Measuring this behavior can enhance behavioral tests. For example, stretch-attend posture is a more sensitive measure of the effects of anxiolytics than traditional spatiotemporal indices. However, quantifying stretch-attend posture using human observers is time consuming, somewhat subjective, and prone to errors. We have developed MATLAB-based software, MATSAP, which is a quick, consistent, and open source program that provides objective automated analysis of stretchattend posture in rodent behavioral experiments. Unlike human observers, MATSAP is not susceptible to fatigue or subjectivity. We assessed MATSAP performance with videos of male Swiss mice moving in an open field box and in an elevated plus maze. MATSAP reliably detected stretch-attend posture on par with human observers. This freely-available program can be broadly used by biologists and psychologists to accelerate neurological, pharmacological, and behavioral studies.Rodent behavioral analysis is often used to assess the effects of pharmaceuticals, implanted devices, or surgical procedures in preclinical research. The development of image analysis tools has enabled researchers to quantitatively assess various rodent behaviors quickly and objectively 1,2 . However, most automated scoring programs track patterns in spatial locomotor exploration and neglect ethological behaviors, such as head dipping and stretch-attend posture (SAP) 1 . Currently, there is no accurate tracking and scoring software that can directly detect SAP 3 . SAP, which is generally associated with anxiety, occurs when the rodent lowers its back, elongates its body, and is either standing still or moving forward very slowly 4 . SAP is a naturally occurring behavior found in rodents, such as mice, etc. that can be reliably intensified by certain experimental paradigms, such as placing the rodent in an open-field test 4,5 . In mice, the SAP behavior occurs when the mouse is undergoing risk-assessment specifically due to an internal exploratory-anxiety conflict. It can also occur under fearful risk-assessment where SAP would be an ambivalent element reflecting an approach-avoidance tendency 4,6 . When SAP is present during a passive avoidance situation, mice approach or avoid the object at nearly equal rates, which indicates they are undergoing risk-assessment during an approach-avoidance conflict 5,6 . SAP is a good identifier for conflict behavior in mice and can be used to evaluate the effects of drugs at reducing these internal conflicts 7 . During exploratory-anxiety conflict situations, SAP can be used as a valid measure of anxiety as anxiolytic drugs have successfully reduced SAP 5,7-9 .SAP has been evaluated in elevated plus maze (EPM) 10 , open field (OF) 11 , rat exposure test 12 , and canopy stretch attend posture test 8 . Increased SAP behavior of rodents near the entrance of the open arms in EPM and along the border of the canopy in the canopy stretch attend po...