Activation of a DRE-binding transcription factor from Medicago truncatula by deleting a Ser/Thr-rich region

Chen, Ji-Ren; Lu, Jingjing; Wang, Tianxiang; Chen, Shouyi; Wang, Huafang

doi:10.1007/s11627-008-9163-9

Cited by 18 publications

(14 citation statements)

References 32 publications

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“…However, the regulatory mechanism of stability by NRD is not clear. Whether this posttranslational regulation is common among other plant species is also unclear, although the removal of a region that corresponds to the DREB2A NRD from MtDREB2A of Medicago truncatula enhanced activity in yeast (Saccharomyces cerevisiae; Chen et al, 2009a).…”

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confidence: 99%

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

et al. 2012

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Soybean (Glycine max) is an important crop around the world. Abiotic stress conditions, such as drought and heat, adversely affect its survival, growth, and production. The DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2 (DREB2) group includes transcription factors that contribute to drought and heat stress tolerance by activating transcription through the cis-element dehydration-responsive element (DRE) in response to these stress stimuli. Two modes of regulation, transcriptional and posttranslational, are important for the activation of gene expression by DREB2A in Arabidopsis (Arabidopsis thaliana). However, the regulatory system of DREB2 in soybean is not clear. We identified a new soybean DREB2 gene, GmDREB2A;2, that was highly induced not only by dehydration and heat but also by low temperature. GmDREB2A;2 exhibited a high transactivation activity via DRE and has a serine/threonine-rich region, which corresponds to a negative regulatory domain of DREB2A that is involved in its posttranslational regulation, including destabilization. Despite the partial similarity between these sequences, the activity and stability of the GmDREB2A;2 protein were enhanced by removal of the serine/threonine-rich region in both Arabidopsis and soybean protoplasts, suggestive of a conserved regulatory mechanism that involves the recognition of serine/ threonine-rich sequences with a specific pattern. The heterologous expression of GmDREB2A;2 in Arabidopsis induced DREregulated stress-inducible genes and improved stress tolerance. However, there were variations in the growth phenotypes of the transgenic Arabidopsis, the induced genes, and their induction ratios between GmDREB2A;2 and DREB2A. Therefore, the basic function and regulatory machinery of DREB2 have been maintained between Arabidopsis and soybean, although differentiation has also occurred.

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confidence: 99%

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

et al. 2012

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“…In our previous study, we isolated a MtDREB2A gene and identified a regulatory domain (central part of MtDREB2A) in the regulation of DREB2A (Chen et al 2009a). In this study, we isolated and characterized a DREB gene from M. truncatula (MtDREB1C), which was a cold-acclimation-specific gene (Pennycooke et al 2008) exhibiting the highest homology to Arabidopsis DREB1C gene.…”

Section: Discussionmentioning

confidence: 99%

“…The function of downstream genes controlled by MtDREB1C associated with freezing stress tolerance in the transgenic China Rose should also be further studied. Although only one surviving line has been obtained and the homolog of DREB in China Rose plants has yet to be identified, we may hypothesize that the China Rose might possess a DER-DREB-based stress response system similar to that in Arabidopsis (Liu et al 1998), rice (Oh et al 2005), wheat (Shen et al 2003a), and Medicago (Chen et al 2009a). With this knowledge, DREBtransformed China Rose lines with higher freezing-tolerance will be obtained in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Transformation was performed according to the previous protocol (Chabaud et al 1996;Chen et al 2009a;Trieu and Harrison 1996) The transformed cotyledon explants were cultured on M3 media containing 5 mg L -1 geneticin. The regenerated and differentiated shoots were harvested for RNA analysis with or without cold treatment.…”

Section: Plant Materials and Plant Expression Vectors Used For Agrobamentioning

confidence: 99%

“…In order to characterize the putative regulatory molecules that are unique to salt/drought or cold/freezing stresses, we used an in silico approach to isolate two DRE-binding transcription factor genes from M. truncatula. The first is a droughtinducible gene, namely, MtDREB2A (Chen et al 2009a) and the other is MtDREB1C (GenBank accession no. DQ267620).…”

Section: Introductionmentioning

confidence: 99%

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DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.)

Chen

Liu

et al. 2010

Plant Growth Regul

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We isolated a DREB orthologue, MtDREB1C, from Medicago truncatula. Its deduced protein contains an AP2 domain of 57 amino acids. Yeast one-hybrid assay revealed that MtDREB1C specifically bound to the dehydration-responsive element (DRE) and activated the expression of HIS3 and LacZ reporter genes. In a transcriptional activation assay, coexpression of the MtDREB1C cDNA resulted in much higher (21.2 times) transactivation of the LacZ reporter gene than experiments performed without MtDREB1C. Transformation of Medicago revealed that overexpression of MtDREB1C suppressed shoot growth, and enhanced the freezing tolerance of M. truncatula. The MtDREB1C gene was transformed into China Rose (Rosa chinensis Jacq.) driven by Arabidopsis rd29A promoter. Southern-blot analysis showed that the target gene was integrated into the genome of a surviving transgenic rose plant. Northern-blot analysis illustrated that robust expression of MtDREB1C was only activated under stress conditions, and the expressed MtDREB1C mRNA reached maximum accumulation 10 h following freezing treatment. The performance of the transgenic line under freezing stress was superior to untransformed controls. This transgenic plant continued to grow, flowered under unstressed conditions, and was phenotypically normal. These facts indicate that the MtDREB1C gene, isolated from Medicago truncatula and driven by the Arabidopsis rd29A promoter, enhanced freezing tolerance in transgenic China Rose significantly without any obvious morphological or developmental abnormality.

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Co-expression of MtDREB1C and RcXET Enhances Stress Tolerance of Transgenic China Rose (Rosa chinensis Jacq.)

Chen

Ziemiańska

et al. 2016

J Plant Growth Regul

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Activation of a DRE-binding transcription factor from Medicago truncatula by deleting a Ser/Thr-rich region

Cited by 18 publications

References 32 publications

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

GmDREB2A;2, a Canonical DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN2-Type Transcription Factor in Soybean, Is Posttranslationally Regulated and Mediates Dehydration-Responsive Element-Dependent Gene Expression

DREB1C from Medicago truncatula enhances freezing tolerance in transgenic M. truncatula and China Rose (Rosa chinensis Jacq.)

Co-expression of MtDREB1C and RcXET Enhances Stress Tolerance of Transgenic China Rose (Rosa chinensis Jacq.)

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